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  • 1
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  24. Jahrestagung der Deutschen Gesellschaft für Arterioskleroseforschung; 20100318-20100320; Blaubeuren; DOC10dgaf11 /20110323/
    Publication Date: 2011-03-23
    Description: Resveratrol, a polyphenol mainly present in grapes and red wine, demonstrated interesting biomedical properties for its cardio protective action due to inhibition of the oxidation of low-density lipoprotein (LDL) and of platelet aggregation, inhibitory effects on cancer promotion and propagation and anti-inflammatory activities. These potential therapeutic and prophylactic applications are limited by the low bioavailability caused by its physical chemical properties. Additionally, resveratrol has low water solubility and stability making its clinical success a formidable technological and medical challenge. Liposomal formulations have been proposed as a means of improving the therapeutic efficacy of poorly bioavailable drugs. The aim of the study was to determine amount of resveratrol loaded into liposomes in presence of vitamin C and vitamin E. Liposome preparations containing pure resveratrol, resveratrol with vitamin C and resveratrol with vitamin E were prepared using thin film hydration method. Resveratrol content was analyzed using HPLC with UV/DAD detection. Resveratrol was detected in all analyzed samples. Results showed that increase in amount of resveratrol used for preparation of liposomes, lead to increase of amount of resveratrol loaded into liposomes. Also, addition of natural antioxidants such as vitamin C and vitamin E increased incorporated amount of resveratrol.
    Keywords: ddc: 610
    Language: English
    Type: conferenceObject
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  • 2
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  24. Jahrestagung der Deutschen Gesellschaft für Arterioskleroseforschung; 20100318-20100320; Blaubeuren; DOC10dgaf10 /20110323/
    Publication Date: 2011-03-23
    Description: Resveratrol, a polyphenol present in red wine, has been thought to be responsible for cardiovascular benefits associated with moderate wine consumption. The mechanism of cardiovascular benefits probably includes vasorelaxation, antioxidant and anti-platelet effects of resveratrol. The mechanisms by which resveratrol causes vasodilatation are uncertain. The aim of this study was to investigate the mechanism (s) of resveratrol-induced vasorelaxation in rat renal artery (RA) with endothelium. RA rings were precontracted with phenylephrine. Resveratrol induced relaxation of the RA rings. Highly selective blocker of ATP-sensitive K+ channels, glibenclamide as well as selective antagonist of voltage-gated K+ (Kv 1.1.-1.6) channels, margatoxin did not block resveratrol-induced relaxation of RA. A nonselective blocker of Kv channels, 4-aminopyridine and a blocker of big Ca-sensitive K+ (BKCa) channels, charybdotoxin inhibited endothelium-dependent relaxation of RA induced by resveratrol. Resveratrol (100 µM) completely relaxed renal artery precontracted with K+-rich solution. In conclusion, we have shown that resveratrol induces relaxation of RA with endothelium. The KV and BKCa channels are involved in this relaxation, but K+ channel- independent mechanism of action also take a part in its vasorelaxant effect.
    Keywords: ddc: 610
    Language: English
    Type: conferenceObject
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  • 3
    ISSN: 1432-0533
    Keywords: Cerebral post-ischemia ; Gerbil ; Cerebral cortex ; Basal ganglia ; Monoamines ; Monoamine oxidase ; Catechol-O-methyl transferase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The post-ischemic effects on cerebral cortex and basal ganglia monoamine levels and monoamine oxidase (MAO A and B) and catechol-O-methyl transferase (COMT) activities were evaluated in Mongolian gerbils (Meriones unguiculatus) subjected to bilateral common carotid arteries occlusion for 15 min and reflow for 7 days. Disorders of monoamine metabolism was found in ischemic brain which persisted during the long-term post-ischemia. A rebound increase of norepinephrine and serotonin appeared in early stages (up to 1 h) of post-ischemia both in cerebral cortex and basal ganglia; a rebound increase of dopamine was found only in cerebral cortex. Thereafter, the serotonin level was enhanced over the control level during the whole post-ischemic period whereas the levels of catecholamines were reduced particularly in basal ganglia. With respect to monoamine content and activities of monoamine degraded enzymes an oscillatory behavior was observed in post-ischemia. Disorder of the monoamine metabolism found during post-ischemic period possibly contributes to neurological dysfunction after an ischemic insult.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The hopanoid content of the two methanotrophic bacteria Methylocaldum szegediense and Methylocaldum tepidum was investigated. 35-Aminobacteriohopane-30R,31R,32R,33S,34S-pentol and its 3β-methyl homologue were present in both strains. In M. tepidum, they were accompanied by 35-aminobacteriohopane-31R,32R,33S,34S-tetrol and its 3β-methyl homologue. The side chain structure was identical to those previously reported from two other obligate methanotrophs, Methylococcus capsulatus and Methylomonas methanica. The two Methylocaldum species shared with the Methylococcus species the presence of 3β-methylhopanoid as well as of a hopanoid releasing adiantol upon H5IO6/NaBH4 treatment. A rare feature was in addition found in M. szegediense. The saturated hopanoids were accompanied by an unsaturated aminobacteriohopanepentol with a Δ11 double bond. Comparison of the hopanoid fingerprints was in accordance with the close phylogenetic relationship of Methylococcus and Methylocaldum. The major difference was the absence of sterols in Methylocaldum which were always detected in the Methylococcus species.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Hopanoid fingerprints allowed to differentiate bacteria formerly connected to the genus Pseudomonas. Whereas all strains related to Pseudomonas and Ralstonia were devoid of any detectable hopanoid, these pentacyclic triterpenoids were found in the Burkholderia species and in related soil isolates, which contained as main hopanoid a bacteriohopanetetrol carbapseudopentose ether, accompanied by significant amounts of its novel Δ6 unsaturated homologue. Unsaturated hopanoids represent an extremely rare feature in soil bacteria and the only known indication for a catabolism of this pentacyclic carbon skeleton in bacteria.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-7365
    Keywords: cerebral ischemia ; synaptosomes ; 5-hydroxytryptamine ; uptake ; release
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Cerebal ischemia induced by bilateral common carotid artery occlusion (15min) with and without release (1 hr) served as a model for comparative regional studies of synaptosomal 3H-5-hydroxytryptamine (3H-5-HT) uptake and release in adult and young gerbils. A decreased uptake and an increased release of 5-HT was observed in the adult after ischemia alone and/or ischemia with reflow. At the same time, 5-HT uptake was not affected except in the cortex and the release was reduced in the young. These findings indicate that the same ischemic insults affect differently the synaptosomal uptake and/or release of 5-HT in adult and young brain.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Galectin-3 is a beta-galactoside binding protein involved in multiple biological processes through interactions with complementary glycoconjugates. We analysed the expression and coexpression of galectin-3 and carcinoembryonic antigen (CEA), one of the putative galectin-3 ligands, in medullary thyroid carcinoma (MTC).An immunohistochemical study using monoclonal antibodies was performed on paraffin sections of 20 cases of sporadic MTC comprising 10 cases without and 10 cases with lymph node metastases at the time of surgery. CEA expression was found in all tumours, distributed predominantly in the cytoplasm and occasionally at the cell surface. In the majority of cases (18/20) moderate to strong intensity of staining was found in most of the cells. Positive cytoplasmic staining for galectin-3 was found in 16/20 cases, but varied in intensity and distribution from weak/focal (7/16) to moderate (7/16) or strong (2/16). More intense staining for galectin-3 was mainly associated with MTC cases involving lymph node metastases. Eight out of these 10 cases showed moderate to strong galectin-3 expression concomitant with CEA expression throughout the tumour tissue.These findings suggest that galectin-3 might play a role in the pathobiology of MTC. Simultaneous expression of galectin-3 and CEA in the same tumour cells at an advanced stage of MTC indicates the possibility of their autocrine cooperation during tumour progression.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aims : Galectin-3 is a β-galactoside binding protein, recently recognized as a promising molecular marker of thyroid malignancy. As reported in several studies, galectin-3 is highly expressed in papillary thyroid carcinoma, but its expression has not been investigated in papillary microcarcinoma, which is a variant of papillary thyroid carcinoma.Methods and results : Using a monoclonal antibody to galectin-3 and the avidin–biotin–peroxidase complex (ABC) immunohistochemical technique, we analysed galectin-3 expression in 63 cases of papillary microcarcinoma. The results showed immunohistochemical reactivity for galectin-3 in 51 (80.9%) cases. Intensity of staining varied from strong or moderate to weak. Galectin-3 localization was mostly cytoplasmic, but also membranous or nuclear in some cells. Immunohistochemical expression of galectin-3 was not found in 12 (19.1%) cases. Most galectin-3 negative microcarcinomas (10/12) were of the non-classical type, i.e. without papillary architecture. Neither the frequency nor the intensity of a positive reaction was related to tumour size.Conclusions : Galectin-3 gene is expressed at the protein level in most papillary microcarcinomas, although with slightly lower frequency than that reported for clinically evident papillary thyroid carcinoma. The presence of galectin-3 in clinically silent microcarcinomas may indicate that galectin-3 is not related to growth or aggressiveness of papillary thyroid microcarcinomas but rather plays some other role in thyroid tumour biology.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-6903
    Keywords: Cerebral ischemia ; 5-hydroxytryptamine ; S2-receptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A possible relationship between the changes in the properties of S2-receptor binding sites and the turnover rate of 5-hydroxytryptamine (5-HT) has been investigated in cerebral ischemia associated with either little or marked edema. Bilateral common carotid artery occlusion for 5 or 15 min with 1 hour of reestablished blood flow in gerbils served as a model for the respective studies. An alteration in kinetic characteristics of S2-receptor binding sites labeled with [3H]ketanserin (the potent 5-HT antagonist for postsynaptic receptors) was detected in the synaptosomes separated from brains of gerbils subjected to 1 hour release after 15 but not following 5 min of bilateral ischemia. At the same time, an increased turnover rate of 5-HT was found in the cerebro-cortical homogenate. The duration of ischemic insult which leads to the changes in the properties of S2-binding sites and the increase in turnover rate of 5-HT has been identical with that needed for the marked accumulation of water in the gerbil brain reported previously. Thus, these findings are consistent with the implicated involvement of 5-HT in the formation of ischemic cerebral edema.
    Type of Medium: Electronic Resource
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  • 10
    Publication Date: 2018-07-18
    Description: Anna M. Ranzoni and Ana Cvejic In March 2018, over 250 researchers came together at the Wellcome Genome Campus in Hinxton, Cambridge, UK, to present their latest research in the area of single-cell biology. A highly interdisciplinary meeting, the Single Cell Biology conference covered a variety of topics, ranging from cutting-edge technological innovation, developmental biology and stem cell research to evolution and cancer. This meeting report summarises the key findings presented and the major research themes that emerged during the conference.
    Keywords: Stem cells & regeneration
    Print ISSN: 0950-1991
    Electronic ISSN: 1477-9129
    Topics: Biology
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