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  • 1
    Abstract: Leukaemogenesis requires enhanced self-renewal, which is induced by oncogenes. The underlying molecular mechanisms remain incompletely understood. Here, we identified C/D box snoRNAs and rRNA 2'-O-methylation as critical determinants of leukaemic stem cell activity. Leukaemogenesis by AML1-ETO required expression of the groucho-related amino-terminal enhancer of split (AES). AES functioned by inducing snoRNA/RNP formation via interaction with the RNA helicase DDX21. Similarly, global loss of C/D box snoRNAs with concomitant loss of rRNA 2'-O-methylation resulted in decreased leukaemia self-renewal potential. Genomic deletion of either C/D box snoRNA SNORD14D or SNORD35A suppressed clonogenic potential of leukaemia cells in vitro and delayed leukaemogenesis in vivo. We further showed that AML1-ETO9a, MYC and MLL-AF9 all enhanced snoRNA formation. Expression levels of C/D box snoRNAs in AML patients correlated closely with in vivo frequency of leukaemic stem cells. Collectively, these findings indicate that induction of C/D box snoRNA/RNP function constitutes an important pathway in leukaemogenesis.
    Type of Publication: Journal article published
    PubMed ID: 28650479
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  • 2
    Abstract: The recent advent of methods for high-throughput single-cell molecular profiling has catalyzed a growing sense in the scientific community that the time is ripe to complete the 150-year-old effort to identify all cell types in the human body. The Human Cell Atlas Project is an international collaborative effort that aims to define all human cell types in terms of distinctive molecular profiles (such as gene expression profiles) and to connect this information with classical cellular descriptions (such as location and morphology). An open comprehensive reference map of the molecular state of cells in healthy human tissues would propel the systematic study of physiological states, developmental trajectories, regulatory circuitry and interactions of cells, and also provide a framework for understanding cellular dysregulation in human disease. Here we describe the idea, its potential utility, early proofs-of-concept, and some design considerations for the Human Cell Atlas, including a commitment to open data, code, and community.
    Type of Publication: Journal article published
    PubMed ID: 29206104
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  • 3
    ISSN: 1617-4623
    Keywords: Keywords CryIC ; Spodoptera littoralis ; δ-Endotoxin ; Proteolytic processing ; Site-specific mutagenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Insecticidal CryI protoxins of Bacillus thuringiensis are activated by proteolysis in the midgut of insects. A conservation of proteolytic cleavage sites in the CryI proteins facilitates the expression of active toxins in transgenic plants to obtain protection from various insects. However, the engineering of CryIC toxins has, thus far, failed to yield applicable resistance to armyworms of Spodoptera species representing common insect pests worldwide. To improve the production of recombinant CryIC toxins, we established a CryIC consensus sequence by comparative analysis of three cryIC genes and tested the stability and protease sensitivity of truncated CryIC toxins in Escherichia coli and in vitro. In contrast to previous data, the boundaries of trypsin-resistant CryIC core toxin were mapped to amino acid residues I28 and R627. Proteolysis of the truncated CryIC proteins showed that Spodoptera midgut proteases may further shorten the C-terminus of CryIC toxin to residue A615. However, C-terminal truncation of CryIC to residue L614, and a mutation causing amino acid replacement I610T, abolished the insecticidal activity of CryIC toxin to S. littoralis larvae, as well as its resistance to trypsin and Spodoptera midgut proteases. Because no CryIC toxin carrying a proteolytically processed N-terminus could be stably expressed in bacteria, our data indicate that, in contrast to other CryI poteins, an entomocidal fragment located between amino acid positions 1 and 627 is required for stable production of recombinant CryIC toxins.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-4846
    Keywords: chromatography ; foams ; macroreticular
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A new method for the formation of reticular silicate and organically modified silicate is introduced. Monoliths were prepared by incorporating a few percent hydrogen peroxide in the sol-gel starting solution. For example, incorporation of 6–10% (v) hydrogen peroxide in base catalyzed sol-gel precursors of methyl-Ormosil yielded macroporous monoliths with a bi-modal pore size distribution. The average characteristic pore diameters were approximately 1.2 nm and 0.7 μm, depending on the sol-gel precursors used and the preparation protocol. The specific surface area was approximately 160 m2/g, contributed mainly by the microporous structure. A similar preparation procedure without hydrogen peroxide yielded only fractured or powdery materials. Presumably, the decomposition of the hydrogen peroxide yielded microbubbles, which formed templates for the polycondensation reaction. SEM, nitrogen adsorption isotherms and small angle X-ray spectroscopy were used to characterize the reticular materials.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1435-4373
    Keywords: Necrotizing fasciitis ; Staphylococcus aureus ; Bacteremia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Two patients with rapidly progressive necrotizing fasciitis of a lower extremity due toStaphylococcus aureus as a single pathogen are described. In both patients the portal of entry was attributed to needle puncture (intra-articular injection and intravenous catheter, respectively), followed by bacteremia. Necrotizing fasciitis occurred in a site remote from the needle puncture, suggesting metastatic infection. One patient developed toxic shock syndrome and the other a sunburn-like rash and erythematous mucosae with strawberry tongue. One patient died, and the other required above-knee amputation due to secondary infectious complications.Staphylococcus aureus may mimic the presentation of invasive group A streptococcal infections. A history of needle puncture should alert the physician to the possibility ofStaphylococcus aureus infection.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1435-4373
    Keywords: Key words Necrotizing fasciitis ; Staphylococcus aureus ; Bacteremia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Two patients with rapidly progressive necrotizing fasciitis of a lower extremity due to Staphylococcus aureus as a single pathogen are described. In both patients the portal of entry was attributed to needle puncture (intra-articular injection and intravenous catheter, respectively), followed by bacteremia. Necrotizing fasciitis occurred in a site remote from the needle puncture, suggesting metastatic infection. One patient developed toxic shock syndrome and the other a sunburn-like rash and erythematous mucosae with strawberry tongue. One patient died, and the other required above-knee amputation due to secondary infectious complications. Staphylococcus aureus may mimic the presentation of invasive group A streptococcal infections. A history of needle puncture should alert the physician to the possibility of Staphylococcus aureus infection.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5117
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A 4.5 km section of the River Dan in northern Israel was sampled at intervals of 6 weeks, between April 1983–March 1984, using standardized mesh bags. The river is characterized by extremely stable temperature and other physical and chemical conditions. The effects of a water diversion project 2400 m from the spring were investigated. The relationships between fauna and depth, current velocity and distance from the spring sources were evaluated. Of 48 common taxa analyzed, 27 showed relativity to the distance from the sources, 18 to current velocity and 12 to depth. The lowest density of invertebrates, but the highest number of taxa and highest variety of species were found at the spring sources. The sampling site immediately downstream from the water diversion project was characterized by intermediate densities, lowest varieties of species and highest evenness of distribution. Results are compared with the predictions of the Intermediate Disturbance Hypothesis; but the influence of invertebrate drift caused this hypothesis to be inapplicable to lotic waters. Invertebrate drift is in addition suggested as an important factor governing the structure of the fauna at the spring sources.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 160 (1988), S. 155-163 
    ISSN: 1573-5117
    Keywords: invertebrate drift ; aquatic insects ; river ecology ; Gammarus ; Baetidae ; environmental impact ; Israel ; Jordan River
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Dan river, a principal source of the Jordan River, Israel, is unusually constant in discharge (∼8 m3·s−1) and water temperature (15–16 °C). The Jordan headwaters constitute the southernmost oasis of a palearctic north temperate fauna, and presumably the very constancy of the Dan contributes to its important role as a regional refuge. However, little is known of river ecology from this region. We report a twelve month study of drift, undertaken to assess diel, seasonal, and spatial patterns of the abundance of drifting invertebrates. Diel periodicity in drift was detectable but minimal. Baetidae nymphs showed a pronounced nocturnal increase, gammarid amphipods a modest, twofold increase, while dipteran larvae showed no diel variation. Seasonal variation likewise was minimal and due principally to the Baetidae, while gammarid amphipods showed no significant seasonality. The notably small diel and seasonal variation in aquatic drift in the Dan may be attributable to the extremely constant physical regime. Spatial variation was substantial. Two stations located 30 and 200 m below the karstic exsurgence of the Dan provided drift densities among the lowest reported anywhere, whereas two stations located 1 and 4.5 km downstream had more typical drift densities. A water diversion project completed halfway through the study resulted in a 50% reduction in flow at the most downstream stations, but had no discernible effect on drift.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1570-7458
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Quatre variétés commerciales de fraisiers ont été testées quant à leur teneur en farnesol et au nombre d'Acariens hébergés (Tetranychus cinnabarinus). La variété la plus résistante, Tioga, avait la plus faible teneur en farnesol, tandis que la variété la plus sensible, Fresno, avait la teneur la plus élevée. Les deux autres variétés, Aliso et Sequoia, étaient intermédiaires quant à leur teneur en farnesol et à leur degré d'infestation.
    Notes: Abstract Numbers of the carmine spider mite, Tetranychus cinnabarinus and concentration of the sesquiterpene alcohol farnesol were assayed for four commercial varieties of strawberry. The most resistant variety. Tioga, had the lowest farnesol content, whereas the most susceptible variety, Fresno, had the highest. The other two varieties: Aliso and Sequoia were intermediate in regard to mite infestation as well as farnesol content.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Astrophysics and space science 132 (1987), S. 249-256 
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The hydrodynamics of stellar models in the tidal field of a massive black hole is calculated numerically under certain simplifying assumptions. It is found that solar type stars are totally disrupted only when falling toward a black hole whose mass does not exceed 106–107 M ⊙. Red giants lose their entire envelope for all black hole masses studied. Our findings strengthen the view that tidal disruption is not an important source of gas in AGN's.
    Type of Medium: Electronic Resource
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