Springer Online Journal Archives 1860-2000
Chemistry and Pharmacology
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Abstract Two water-in-oil microemulsion systems formulated with lecithin and AOT were used to solubilize lipase fromPseudomonas cepacia. The site of localization of the enzyme within the different microdomains of the dispersed phase was investigated by applying the fluorescence energy transfer technique. The nonradiative energy transfer from the tryptophan residues of lipase to an adequate acceptor molecule, such ascis-parinaric acid, was studied in both systems. The effect of the water content of the microemulsions, as well as the effect of the molar ratio of lipase tocis-parinaric acid was examined. The results showed that in both microemulsion systems lipase is preferentially localized near the surfactant membrane and that in the case of the lecithin-based systems the interface is less penetrable than the AOT one.
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