Platelet-derived growth inhibitor
transforming growth factor-β type 1
bovine aortic endothelial cells
Springer Online Journal Archives 1860-2000
Abstract In 1986, Brown and Clemmons (Proc. natl Acad. Sci. USA83 (1986) 3321) showed that platelets contain a substance, platelet-derived growth inhibitor (PDGI), that inhibits in vitro endothelial cell replication. Although platelets are rich in transforming grwoth factor β (TGF-β), PDGI was considered not to be related to TGF-β, on the basis of its reported properties (extraction from platelets at neutral pH, binding to heparin-Sepharose). However, we purified PDGI to near homogeneity and showed that on the basis of HPLC retention behavior, in vitro growth inhibitory activities with several cell types, receptor binding, and immunoneutralization of growth inhibitory activity with specific anti-TGF-β type 1 antibodies, PDGI is most probably identical with TGF-β type 1.
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