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  • 1
    Keywords: RECEPTOR ; CELLS ; EXPRESSION ; IN-VITRO ; CELL ; SYSTEM ; TOOL ; GENE ; cell line ; TRANSDUCTION ; INFECTION ; murine ; recombination ; antibodies ; antibody ; TARGET ; virus ; MOUSE ; hormone ; VECTORS ; CELL-LINE ; FUSION ; LINE ; CARCINOMA-CELLS ; MAMMALIAN-CELLS ; HEMATOPOIETIC PROGENITOR CELLS ; RETROVIRAL VECTORS ; VIRAL VECTORS ; PLASMID DNA ; TRANSGENE EXPRESSION ; HIGH-TITER ; gene transfer,ES cells,MESV retroviral vectors,adenoviral vectors,Cre recombinase,Cre.PR fusion ; RECOMBINANT ADENOVIRUS ; SOMATIC MUTAGENESIS
    Abstract: Background Genetic modification of embryonic stem (ES) cells represents a powerful tool for transgenic and developmental experiments. We report that retroviral constructs based on murine embryonal stem cell virus (MESV) can efficiently deliver and express Cre recombinase or a post-translationally inducible Cre-Progesterone receptor (Cre.PR) fusion in mouse fibroblasts and ES cells.Methods To study the vectors a sensitive reporter cell line, 3TZ, was derived from the murine 3T6 fibroblast line that expresses beta-galactosidase only upon Cre-mediated recombination. This was used together with the ROSA26-R ES cell Cre-reporter system or unmodified mouse ES cells as targets of infection. Efficiency of gene transfer was evaluated immunohistochemically by the use of an anti-Cre polyclonal antibody, and by monitoring the expression of beta-galactosidase.Results Infection of the 3TZ cells with high titer 718C or 719CP virus revealed efficient gene transduction of constitutive or hormone-inducible recombinase activity, respectively. The vectors efficiently transduced murine ES cells with Cre, Cre-PR (fusion of Cre and progesterone receptor) or beta-galactosidase. Cre-mediated recombination in more than 60% of ROSA26-R ES cells was achieved when infected by a VSV-G-pseudotyped MESV retrovirus at MOI of 50.Conclusions The MESV-based retroviral systems, when combined with hormone inducible Cre, represent efficient tools for the transfer of Cre activity in ES cells. Copyright (C) 2004 John Wiley Sons, Ltd
    Type of Publication: Journal article published
    PubMed ID: 14716675
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  • 2
    Abstract: Regulatory T (Treg) cells that express the Foxp3 transcription factor are essential for lymphoid homeostasis and immune tolerance to self. Other nonimmunological functions of Treg cells, such as controlling metabolic function in adipose tissue, are also emerging. Treg cells originate primarily in the thymus, but can also be elicited from conventional T cells by in vivo exposure to low-dose antigen or homeostatic expansion or by activation in the presence of TGFbeta in vitro. Treg cells are characterized by a distinct transcriptional signature controlled in part, but not solely, by Foxp3. For a better perspective on transcriptional control in Treg cells, we compared gene expression profiles of a broad panel of Treg cells from various origins or anatomical locations. Treg cells generated by different means form different subphenotypes and were identifiable by particular combinations of transcripts, none of which fully encompassed the entire Treg signature. Molecules involved in Treg cell effector function, chemokine receptors, and the transcription factors that control them were differentially represented in these subphenotypes. Treg cells from the gut proved dissimilar to cells elicited by exposure to TGFbeta in vitro, but instead they resembled a CD103(+)Klrg1(+) subphenotype preferentially generated in response to lymphopenia.
    Type of Publication: Journal article published
    PubMed ID: 20231436
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  • 3
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Conditions have been established for the separation of viable mouse lymphoid cells by continuous free-buffer film preparative electrophoresis. The detailed electrophoretic distribution profiles of T and B lymphocytes from mouse spleen and thoracic duct have been determined. Cell surface θ-antigen was used as a marker for T cells, and high surface-density of immunoglobulin as a marker for B cells. Spleen cells from athymic “nude” mice were also studied.In the unselected normal spleen cell populations B lymphocytes are heterogeneous, about 60% being of low mobility with the remainder distributing broadly, and extending into the highest mobility fractions. T lymphocytes are predominantly of high mobility. Lymphoid cells lacking markers of either the B or T lineage are of intermediate mobility. There is only partial separation of T and B cells because of the extensive overlap between the populations.The high mobility B cells, which separate along with T cells, include a substantial proportion of large cells, and include cells with high surface density of immunoglobulin. The majority of these large B cells can be selectively eliminated by their adherence on passage through a glass-bead column.By pre-selecting the 50% non-adherent lymphocytes from spleen as the starting material, a very sharp and more extensive separation of B and T cells can be achieved, with 100% pure B cells and 90% pure T cells in many fractions. However these samples are not representative of the total T and B cell populations of spleen.In thoracic duct lymph high mobility B-cells are absent, there is little overlap between T and B cell mobility. 100% pure T and B cells can be isolated.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1434-6079
    Keywords: 36.40 ; 31.20
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Potential curves for the ground (2Σ u + ) and the three lowest excited states of the Xe 2 + dimer ion (2Π g ,2Π u ,2Σ g + ) have been calculated using pseudopotentials in MRD-CI (multi-reference single anddouble excitationconfigurationinteraction) calculations. Spin-orbit interaction — leading to the six states 1.(1/2) u , 1.(3/2) g , 1.(3/2) u , 1.(1/2) g , 2.(1/2) u , 2.(1/2) g — has been taken into account using a semiempirical technique [1]. Subsequently, starting with a relaxed Xe 2 + ion in its ground state, the potential energy surface for the system Xe-Xe 2 + was studied. We found that the collinear approach of the Xe atom leads to the most stable geometry. This is a linear symmetric molecule with bond lengths of 6.38 bohr. In the bestT-shaped structure, the Xe atom is 7.83 bohr away from the midpoint of the Xe 2 + (r=6.1 bohr) dimer. The calculated binding energy of 0.25 eV for the equilibrium structure of the Xe 3 + molecule (i.e. the linear symmetric geometry), is in very good agreement with experimental results of 0.27 ± 0.02 eV [2].
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1434-6079
    Keywords: 31.20.T ; 36.40
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Monte-Carlo calculations have been performed for positively charged argon clusters in the temperature range between 10 K and 40 K using two different models (one with a dimer ion core, the other one with a trimer ion). The argon-argon interaction potential stems from empirical data, the ion-neutral atoms potential is determined by ab initio MRD-CI calculations. Special stability is found for clusters sizesn=13, 19, 23 and 25/26 atoms using the ‘trimeric core model’ and for those withn=14,n=17,n=20 using the ‘dimeric core model’. The geometrical structure of the clusters is given and the construction principles are discussed in light of the interactions among neutral argon atoms and the ion-neutral atoms interaction.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1434-6079
    Keywords: 31.20.T ; 36.40
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Monte-Carlo calculations have been performed for positively charged xenon-argon clusters in the temperature range between 10K and 40K for cluster sizes up ton=27. The argon-argon interaction potential stems from empirical data, the Xe+-Ar potential is determined by ab initio MRD-CI calculations and a semi-empirical treatment of spin-orbit effects. Special stability is found for cluster sizesn=10, 13, 19 and less pronounced forn=23 and 25 fairly independent of the temperature. The geometrical structure of the clusters are given and the construction principle is discussed in light of the interactions among neutral argon atoms and the xenon ion — argon interaction. Comparison with measured mass spectra for mixed rare-gas clusters and [Xen]+ clusters is made and shows a consistent picture for the building principle.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1434-6079
    Keywords: 36.40 ; 31.20
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Configuration interaction calculations are carried out to study the potential energy surface for the system Ar-Ar 2 + . An all-electron as well as a pseudopotential treatment is employed. It is found that in the perpendicular Ar approach the Ar 2 + partner remains essentially unchanged and the potential can be characterized by an electrostatic ion-induced dipole interaction. In the collinear mode of Ar approach the Ar 2 + bond separation increases considerably, the charge is redistributed and the interaction can be characterized as chemical bonding. The minimum on the surface is found to be the linear symmetric molecule with bond lengths of 2.62 Å. The optimum structure in the perpendicular approach lies 0.13 eV above the minimum and is the T-shaped molecule in which the Ar is 3.65 Å away from the midpoint of the Ar 2 + (r=2.46 Å) system; the best equilateral triangle structure has a bond length of 2.99 Å but is found to lie 0.64 eV above the Ar 3 + minimum. The dissociation energy into Ar 2 + + Ar is calculated to be 0.16 eV in reasonable agreement with experimental values of 0.21 eV. The potential curves for the four lowest states of Ar 2 + are also treated.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Physics of Plasmas 2 (1995), S. 4369-4371 
    ISSN: 1089-7674
    Source: AIP Digital Archive
    Topics: Physics
    Notes: In a first step toward generating an electron–positron plasma, a proof-of-principle experiment is reported in which externally injected slow positrons are trapped in a magnetic mirror configuration by electron cyclotron resonance heating. With a primary flux of only 530 slow positrons/s from a 600 μCi Na-22 positron source/moderator system, an estimated equilibrium density of 5×102 cm−3 is obtained in a 20 cm3 volume. With an appropriate increase of the injected positron flux, densities in the 107 cm−3 range can be expected. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Physics of Fluids 28 (1985), S. 3099-3106 
    ISSN: 1089-7666
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The lifetime of energetic electrons generated via second harmonic electron-cyclotron-resonance heating in a symmetric magnetic mirror configuration is investigated experimentally. The decay time is observed to be as much as a factor of 4 shorter than the value calculated for the most rapid classical loss mechanism, Coulomb drag. The nonclassical loss component is shown to be induced by the microwave power available for electron heating. The microwave power required to sustain the temperature and density of the energetic electrons is calculated from the observed lifetimes and stored energy. It is found to be only a few percent of the total injected power which, in this experiment, is equal to or less than 1 kW.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1279-8517
    Keywords: Angiography ; Technology ; Aortic arch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé A partir d'une étude anatomique de 46 spécimens, les angles du tronc artériel brachio-céphalique droit et de l'artère sous-clavière gauche par rapport à la tangente à la crosse de l'aorte sont mesurés de façon à déterminer quelle voie permet l'accès le plus facile et le plus rapide à l'aorte descendante lors des angiographies des membres inférieurs en cas d'artériopathie sévère. L'étude montre que les angles moyens (77.1±16.0 degrés pour le tronc brachio-céphalique, 77.7±16.3 degrés pour l'artère sous-clavière gauche) ne sont pas significativement différents. Comme l'abord axillaire droit comporte plus de risques qui ne peuvent être contre-balancés que par un accès plus rapide, nous concluons que chez les patients de tous âges, le côté gauche droit être la voie d'accès de première intention.
    Notes: Summary In an anatomic study of 46 specimens, the angle of the brachiocephalic trunk and the left subclavian artery to the tangent of the aortic arch was measured in an effort to decide which route offers the quickest access to the descending aorta in lower extremity angiography for severe occlusive vascular diseases. The study shows that the average angles (77.1±16.0 degrees for the brachiocephalic trunk/77.7±16.3 degrees for the left subclavian artery) are not significantly different. Since the right axillary approach incurs other risks that could only be compensated by significantly quicker access, i.e. shorter maneuvering time in the aortic arch we conclude that in patients of all ages the left side should be the access route of choice.
    Type of Medium: Electronic Resource
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