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  • 1
    Keywords: Life sciences ; Biochemistry ; Cell receptors ; Life sciences ; Biochemistry, general ; Receptors ; Springer eBooks
    Description / Table of Contents: Introduction.-℗ History of PPAR Discovery:℗ Peroxisomes -- Peroxisomal biogenesis and diseases -- Peroxisome proliferation.-℗ Peroxisome Proliferator-Activated Receptors:℗ Chromosomal location and gene polymorphism -- Protein structure -- PPAR Ligands:℗ Endogenous ligands -- Exogenous PPAR modulators -- Tissue Distribution and Versatile Functions of PPARs:℗ Neurological functions of PPARs.-℗ PPARs and the cardiovascular system.-℗ PPARs in pulmonary physiology and disease.-℗ Gastrointestinal roles of PPARs.-℗ PPAR functions in the liver.-℗ Roles of PPARs in the pancreas.-℗ PPARs in the urinary tract physiology and pathophysiology.-℗ PPARs in the reproductive system -- Bone metabolism and PPARs.-℗ Roles of PPARs in skeletal muscle biology.-℗ Skin PPARs.-℗ PPARs and Drug Metabolism -- Molecular Aspects of PPAR Actions:℗ Posttranslational control of PPARs.-℗ Mechanism of action.-℗ Animal Models in PPAR Research.-℗ Safety of PPAR Agonists.-℗ The Future of PPAR Research
    Abstract: All three peroxisome proliferator-activated receptor (PPAR) subtypes share a high degree of structural homology while exhibiting differences in function, tissue distribution, and ligand specificity. In Peroxisome Proliferator-Activated Receptors: Discovery and Recent Advances, the authors trace the history of PPAR discovery and detail the receptor structure and its posttranslational modifications. Furthermore, endogenous ligands as well as various classes of exogenous ligands, subtype-selective, dual and pan agonists as well as antagonists, are discussed. In addition, the tissue distribution and versatile functions of PPAR subtypes in major organs are described.℗ As PPARs play critical roles as regulators of numerous physiological as well as pathophysiological pathways, Peroxisome Proliferator-Activated Receptors: Discovery and Recent Advances aims to help researchers to develop safer and more effective PPAR modulators as therapeutic agents to treat a myriad of diseases and conditions
    Pages: : digital.
    ISBN: 9781627034203
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  • 2
    Keywords: Life sciences ; Biochemistry ; Nucleic Acids ; Life sciences ; Biochemistry, general ; Nucleic Acid Chemistry ; Springer eBooks
    Description / Table of Contents: PPARs: History and Advances -- PPAR-Alpha Cloning, Expression and Characterization -- PPARGC1A and PPARG Genotyping. Beginneŕ€™s Guide to Genotyping with Unlabeled Probes -- Generation of an Inducible, Cardiomyocyte-Specific Transgenic Mouse Model with PPARÎø/d Overexpression -- Specific knockdown of PPARd Gene in Colon Cancer Cells by Lentivirus-Mediated RNA Interfering -- Dominant-Negative and Knockdown Approaches to Studying PPAR activity -- Producing PPARgamma2 Knockdown in Mouse Liver -- Adipose Tissue-Specific PPARg Gene Targeting -- Site-Directed Mutagenesis to Study the Role of Specific Amino Acids in the Ligand Binding Domain of PPARs -- PPAR SUMOylation: Some Useful Experimental Tips -- Analyzing Phosphorylation-Dependent Regulation of Subcellular Transcriptional Activity of Transcriptional Coactivator NT-PGC-1a -- In vivo Studies of PPAR-Chromatin Interactions: Chromatin Immunoprecipitation for Single-Locus and Genomewide Analyses -- FISH Analysis Using PPARg℗ Specific Probes for Detection of PAX8-PPARg Translocation in Follicular Thyroid Neoplasms -- Immunohistochemical Techniques to Identify and Localize Proteins of Interest in Paraffin-Embedded Tissue Sections -- Determination of PPAR Expression by Western-Blot -- Fluorescence Resonance Energy Transfer Techniques to Study Ligand-Mediated℗ Interactions of℗ PPARs with Coregulators -- Estimation of the PPARa Agonism of Fibrates by a Combined MM-Docking Approach -- Combined Biophysical and Cell-based Approaches for the Assessment of Ligand Binding to PPARg -- Exploring PPAR Modulation in Experimental Mice -- Induction of Adipogenic Differentiation in Three-Dimensional Culture Model on a Novel Microfabricated Scaffold -- Analyzing PPAa/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry -- Synthesis, Mass Spectrometric Characterization, and Analysis of the PPARd Agonist GW1516 and its Major Human Metabolites ́€“ Targets in Sports Drug Testing -- LC-MS-Based Method for the Qualitative and Quantitative Analysis of the Novel PPARg Agonist KR-62980 -- Behavioral Paradigms to Evaluate PPAR Modulation in Animal Models of Brain Injury
    Abstract: All three peroxisome proliferator-activated receptor (PPAR) subtypes share a high degree of structural homology but differ in function, tissue distribution and ligand specificity. PPARs play critical roles as regulators of numerous physiological as well as pathophysiological pathways, and efforts are currently underway to fully characterize their functioning and to develop safer and more effective PPAR modulators to treat a myriad of diseases and conditions. In Peroxisome proliferator-Activated Receptors: Methods and Protocols, renowned experts in the PPAR arena provide detailed protocols for investigating these receptors. Chapters contain methods ranging from the cloning of receptors to their knockdown, to protocols exploring posttranslational modifications of PPARs and coactivators, as well as receptor subcellular localization. Also assembled are methods to evaluate the involvement of these receptors in behavior functions, an emerging facet in PPAR research. Written in the successful Methods in Molecular Biologý„Ø series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ With its well-honed methodologies, Peroxisome proliferator-Activated Receptors: Methods and Protocols, ℗ will be a useful resource for all seeking to advance their knowledge of this field
    Pages: XI, 343 p. 65 illus., 37 illus. in color. : digital.
    ISBN: 9781627031554
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  • 3
    Keywords: RNA-ligand interactions ; Biochemistry ; Toxicology ; Protein-Ligand Interactions ; Protein Science ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Nuclear Receptors: A Historical Perspective -- An Optimized Immunoblotting Protocol for Accurate Detection of Endogenous PGC-1α Isoforms in Various Rodent Tissues -- Sub-Cellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue -- In Vivo Quantitation of Estrogen Receptor b Subtype Expression in Ovarian Surface Epithelium Using Immunofluorescence Profiling and Confocal Microscopy -- In Vivo ChIP-Seq of Nuclear Receptors: A Rough Guide to Transform Frozen Tissues into High Confidence Genome-Wide Binding Profiles -- High-Content Analysis of Constitutive Androstane Receptor Nuclear Translocation -- Antibody Validation Strategy for Nuclear Receptors -- Immunofluorescence Labeling of Nuclear Receptor Expression in Formalin-Fixed Paraffin-Embedded Tissue -- Detection of ADP-Ribosylation of the Androgen Receptor Using the Recombinant Macrodomain AF1521 from Archaeoglobus fulgidus -- Reconstitution of the Steroid Receptor Heterocomplex -- High-Throughput Imaging of PPIX Using Confocal Microscopy -- PGC-1α Overexpression via Local In Vivo Transfection in Mouse Skeletal Muscle -- A Reverse Transfection Method for Screening of Nuclear Receptor Activators -- Hybrid Reporter Gene Assays: Versatile In Vitro Tools to Characterize Nuclear Receptor Modulators -- Analysis of the Transcriptional Activity of Retinoic Acid-Related Orphan Receptors (RORs) and Inhibition by Inverse Agonists -- Examining the Role of Nuclear Receptors during In Vivo Chemical-Mediated Breast Tumorigenesis -- Analysis of IL-4/STAT6 Signaling in Macrophages -- Isolation and Characterization of Adipose Tissue Macrophages -- Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Mouse Heart Tissues Using Oroboros 2k-Oxygraph -- Generation of Skeletal Myocytes from Embryonic Stem Cells through Nuclear Receptor Signaling -- Obtaining Crystals of PPARγ Ligand Binding Domain Bound to Small Molecules -- Molecular Modeling Approach to Study the PPARγ-Ligand Interactions -- High Dimensional Data Approaches to Understanding Nuclear Hormone Receptor Signaling
    Abstract: This volume presents valuable techniques for studying the class of ligand-activated transcription factors known as nuclear receptors. After a brief overview of the history of the field, chapters cover methods to detect the receptors and their mRNAs in various tissues, protocols to characterize nuclear receptor modulators and activities, their signaling and roles in certain pathogenesis, molecular modeling of nuclear receptor-ligand interactions, as well as the utility of informatics in the field of nuclear receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nuclear Receptors: Methods and Experimental Protocols seeks to aid researchers working toward furthering our understanding of these vital receptors and their role in numerous pathological conditions
    Pages: XII, 314 p. 72 illus., 57 illus. in color. : online resource.
    ISBN: 9781493991952
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  • 4
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-4919
    Keywords: mitochondria ; peroxisomes ; fatty acids metabolism ; coenzyme A deficiency ; pantothenic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Hepatic coenzyme A (CoA) plays an important role in cellular lipid metabolism. Because mitochondria and peroxisomes represent the two major subcellular sites of lipid metabolism, the present study was designed to investigate the specific impact of hepatic CoA deficiency on peroxisomal as well as mitochondrial β-oxidation of fatty acids. CoA deficiency (47% decrease in free CoA and 23% decrease in total CoA) was produced by maintaining weanling male Sprague-Dawley rats on a semipurified diet deficient in pantothenic acid (the precursor of CoA) for 5 weeks. Hepatic mitochondrial fatty acid oxidation of short-chain and long-chain fatty acids were not significantly different between control and CoA-deficient rats. Conversely, peroxisomal poxidation was significantly diminished (38% inhibition) in livers of CoA-deficient rats compared to control animals. Peroxisomal β-oxidation was restored to normal levels when hepatic CoA was replenished. It is postulated that since the role of hepatic mi tochondrial β-oxidation is energy production while peroxisomal β-oxidation acts mainly as a detoxification system, the mitochondrial pathway of β-oxidation is spared at the expense of the peroxisomal pathway when liver CoA plummets. The present study may offer an animal model to investigate mechanisms involved in peroxisomal diseases. (Mol Cell Biochem 175: 37–42, 1997)
    Type of Medium: Electronic Resource
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