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  • 1
    Keywords: INVASION ; PATHWAY ; GROWTH-FACTOR RECEPTOR ; endocytosis ; CELL-MIGRATION ; TUMOR-METASTASIS ; ACTIN ; SRC ; INVADOPODIA FORMATION ; PODOSOMES
    Abstract: Amplified HER2, which encodes a member of the epidermal growth factor receptor (EGFR) family, is a target of effective therapies against breast cancer. In search for similarly targetable genomic aberrations, we identified copy number gains in SYNJ2, which encodes the 5'-inositol lipid phosphatase synaptojanin 2, as well as overexpression in a small fraction of human breast tumors. Copy gain and overexpression correlated with shorter patient survival and a low abundance of the tumor suppressor microRNA miR-31. SYNJ2 promoted cell migration and invasion in culture and lung metastasis of breast tumor xenografts in mice. Knocking down SYNJ2 impaired the endocytic recycling of EGFR and the formation of cellular lamellipodia and invadopodia. Screening compound libraries identified SYNJ2-specific inhibitors that prevented cell migration but did not affect the related neural protein SYNJ1, suggesting that SYNJ2 is a potentially druggable target to block cancer cell migration.
    Type of Publication: Journal article published
    PubMed ID: 25605973
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  • 2
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The toromere, a structure previously reported only in several strains of Drosophila melanogaster, is found in salivary gland nuclei of three populations of Drosophila lummei, a member of the virilis group. The toromere is characterized by being quinacrine-bright and Feulgen-positive. Further staining and enzyme digestion procedures reveal that the toromere is composed of double-stranded DNA with little or no protein complexed with it. The toromere appears as a small quinacrine-bright dot in diploid cells and apparently undergoes polytenization, as it is observed as a large quinacrine-bright doughnut-shaped structure in salivary gland preparations. We describe the effect of larval culture temperature on the morphology and number of toromeres per cell, and lend support to the proposal that the toromere could be a highly replicated episome.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Chromosoma 39 (1972), S. 53-61 
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Using genetically controlled stocks ofDrosophila melanogaster we have compared the frequency of ectopic pairing in a line showing intense quinacrine fluorescence at two sites (81F and 83E) on chromosome 3 with one showing such fluorescence at only one of these sites (81F). The frequency of ectopic pairing is an order of magnitude greater in cells from the line showing intense fluorescence in both regions than in the line showing it in only one. These data indicate that ectopic pairing is dependent upon properties of discrete chromosome regions as small as individual bands. Since A: T-rich chromatin is known to fluoresce intensely after quinacrine staining, these data further suggest that ectopic pairing is dependent on similarities of the DNA of the discrete chromosome regions involved.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 162 (1948), S. 190-191 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] IN a recent communication, Jonxis1 reports quantitative estimations of alkali-resistant and labile hæmoglobin in cases of hæmolytic disease of the newborn. He comes to the conclusion that after birth only hæmoglobin of the labile, adult type is formed, and that in hæmolytic ...
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 233 (1971), S. 190-191 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] (1) Drosophila melanogaster differs consistently from its sibling species D. simulans in the pattern of the brightly fluorescent regions. The two species can be distinguished by the patterns of bright fluorescence of either the mitotic chromosomes of the neuroblasts or the polytene chromosomes of ...
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: There is a strong association between symptomatic gastro-oesophageal reflux and oesophageal adenocarcinoma. With this in mind, the American College of Gastroenterology has recently revised its practice guidelines for the screening of patients with chronic gastro-oesophageal reflux disease (GERD) to identify those at risk of oesophageal adenocarcinoma, and recommends surveillance to identify curable oesophageal neoplasms in patients with established Barrett's oesophagus. Patients  with chronic GERD symptoms, particularly those aged over 50 years, should undergo upper endoscopy. Patients  found to have Barrett's oesophagus should be treated with acid suppression for GERD symptoms and then undergo regular surveillance endoscopy. Surveillance endoscopy every 3 years is recommended for those without dysplasia. For patients with verified low-grade dysplasia, yearly surveillance endoscopy is recommended. For those with focal high-grade dysplasia (defined as high-grade dysplastic changes involving fewer than five crypts), the condition may be followed with endoscopic surveillance performed at 3-month intervals. If there is verified, multifocal high-grade dysplasia, intervention (e.g. oesophagectomy) may be considered. Both observational and computer models suggest a benefit associated with screening and surveillance. Endoscopic screening and surveillance for Barrett's oesophagus compares favourably with mammography for the detection of breast cancer and other accepted medical practices.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Photodynamic therapy involves the activation of an exogeneously administered, or an endogenously gen-erated, photosensitizer with light to produce localized tissue destruction. It is an attractive, predominantly endosopic technique for the palliation of advanced upper gastrointestinal cancer and the eradication of early neoplastic and pre-neoplastic lesions. The nature of the biological response allowing safe healing and the exploitation of tissue threshold effects mean that adjacent tissue damage can be minimized.This review used a database of 368 papers. The nature of the photosensitizer is critical to the depth of tissue damage and the risk of adjacent tissue damage and stricture formation. The generation of protoporphyrin IX following administration of 5-aminolaevulinic acid has proved useful for the treatment of high-grade dysplasia in Barrett’s oesophagus. A double-blind randomized placebo controlled trial has confirmed that it is a safe and effective method for the ablation of low-grade dysplasia. The treatment of more advanced lesions requires exogeneously administered photo-sensitizers. However, recent data indicate that the neoplastic potential remains in some patients and continued follow-up is necessary.Photodynamic therapy can be used to eradicate early neoplasia and palliate advanced cancer, but caution is required before a definitive cure can be claimed.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The patterns of intense fluorescence after staining with quinacrine dihydrochloride were studied in both the polytene chromosomes and mitotic chromosomes of a pair of sibling species, Drosophila melanogaster and D. simulans. Consistent differences between the two species were found in the pattern of fluorescence of both polytene and mitotic chromosomes. In addition, it was discovered that our stock of D. melanogaster (Oregon-R) is polymorphic at one autosomal position for the property of intense fluorescence after quinacrine staining. On the basis of these findings, the usefulness of quinacrine staining in the study of the cytogenetic structure of evolutionarily interesting populations is discussed.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Chromosoma 33 (1971), S. 1-18 
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Amounts of Feulgen staining in individual sperm nuclei of Drosophila melanogaster were determined with a scanning microdensitometer and compared with the DNA-Feulgen levels found for hen erythrocyte nuclei, taken as a presumed cytophotometric standard of 2.5×10−12 g DNA per cell. Under these conditions, the amount of DNA estimated for the haploid male genome of Drosophila was about 0.18×10−12 g DNA, corresponding to a molecular weight of roughly 11× 1010 daltons. From a comparable series of measurements on Feulgen-stained nuclei of hemocytes and epithelial cells of the imaginal soma, values of approximately 0.35–0.36×10−12 g DNA were estimated for the diploid or 2C male genome of this species.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Chromosoma 36 (1972), S. 375-390 
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The pattern of intense fluorescence of interphase nuclei and metaphase chromosomes after staining with quinacrine is described in Samoaia leonensis. Autoradiographic analysis of interphase nuclei after pulse labeling with tritiated thymidine indicates that there is little or no overlap in the time of replication of the intensely fluorescing and weakly fluorescing regions. Autoradiographic analysis of metaphase figures after continuous labeling with tritiated thymidine shows that the intensely fluorescing regions are late replicating and establishes their order of replication. Autoradiographic analysis of interphase nuclei after pulse labeling with tritiated deoxycytidine and of metaphase figures after continuous labeling with this tracer show that there is little, if any, incorporation of deoxycytidine into those chromosome regions which fluoresce intensely after staining with quinacrine and quinacrine mustard. These results indicate that such chromosome regions are characterized chemically by an extremely high, if not exclusive, content of adenine and thymine.
    Type of Medium: Electronic Resource
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