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  • 1
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  Deutscher Kongress für Orthopädie und Unfallchirurgie; 71. Jahrestagung der Deutschen Gesellschaft für Unfallchirurgie, 93. Tagung der Deutschen Gesellschaft für Orthopädie und Orthopädische Chirurgie, 48. Tagung des Berufsverbandes der Fachärzte für Orthopädie; 20071024-20071027; Berlin; DOCW22-1581 /20071009/
    Publication Date: 2007-10-10
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 2
    Keywords: EXPRESSION ; INHIBITOR ; Germany ; KINASE ; GENE ; GENES ; PROTEIN ; SACCHAROMYCES-CEREVISIAE ; transcription ; COMPONENTS ; COMPLEX ; COMPLEXES ; cell cycle ; CELL-CYCLE ; CYCLE ; protein kinase ; PROTEIN-KINASE ; IDENTIFICATION ; gene expression ; protein kinase CK2 ; Saccharomyces cerevisiae ; SUBUNIT ; YEAST ; BUDDING YEAST ; DISRUPTION ; EXPRESSION ANALYSIS ; HOLOENZYME ; PHO pathway
    Abstract: The budding yeast Saccharomyces cerevisiae encounters phosphate starvation by the transcription-regulated PHO pathway. We find that genetic perturbation of protein kinase CK2, a conserved tetrameric Ser/Thr phosphotransferase with links to cell cycle and transcription, affects expression of PHO pathway genes in a subunit- and isoform-specific manner. Remarkably, the genes encoding phosphate supplying phosphatases and transporters are significantly repressed, while the genes encoding components of the central pathway regulator complex, a cyclin-dependent kinase (CDK), a cyclin, and a CDK inhibitor, remain unaltered. Thus, perturbation of CK2 uncouples the executive part of the PHO pathway from its cyclin-CDK control complex. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies
    Type of Publication: Journal article published
    PubMed ID: 12606059
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  • 3
    Keywords: CLONING ; YEAST ; GENETIC SELECTION
    Abstract: The yeast one-hybrid system (1H-system) is applied to studies of transcription-linked DNA-protein interactions under in vivo conditions detected by reporter gene expression. By use of a variant of green fluorescent protein (GFP) as a reporter, the new 1H-system generation represents a time- and work-saving alternative to the established HIS3/lacZ-based form. However, hitherto, a positive control proving the functionality of the system has been missing. We designed a corresponding control vector combination by subcloning mouse p53 cDNA and its binding sequence and, to get the system working, modified the distance between cloning site and reporter gene promoter in the reporter vector by site-directed mutagenesis. This provides, for the first time, a positive control vector combination for the GFP 1H-system, crucial for its employment in any DNA-protein interaction studies.
    Type of Publication: Journal article published
    PubMed ID: 11969195
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  • 4
    Keywords: EXPRESSION ; KINASE ; GENE ; GENE-EXPRESSION ; GENES ; PROTEIN ; PROTEINS ; SACCHAROMYCES-CEREVISIAE ; transcription ; DYNAMICS ; cell cycle ; CELL-CYCLE ; CYCLE ; PHOSPHORYLATION ; protein kinase ; PROGRESSION ; CATALYTIC SUBUNIT ; CHROMATIN ; chromatin remodeling ; DNA TOPOISOMERASE-II ; ENCODES ; gene expression ; GENETIC- ANALYSIS ; HISTONE DEACETYLASE-2 ; MICROARRAY DATA ; protein kinase CK2 ; Saccharomyces cerevisiae ; SUBUNIT ; TRANSCRIPTIONAL ACTIVITY ; YEAST
    Abstract: Protein kinase CK2, a vital, pleiotropic and highly conserved serine/threonine phosphotransferase is involved in transcription-directed signaling, gene control and cell cycle regulation and is suspected to play a role in global processes. Searching for these global roles, we analyzed the involvement of CK2 in gene expression at cell cycle entry by using genome- wide screens. Comparing expression profiles of Saccharomyces cerevisiae wild-type strains with strains with regulatory or catalytic subunits of CK2 deleted, we found significant alterations in the expression of genes at all cell cycle phases and often in a subunit- and isoform-specific manner. Roughly a quarter of the genes known to be regulated by the cell cycle are affected. Functionally, the genes are involved with cell cycle entry, progression and exit, including spindle pole body formation and dynamics. Strikingly, most CK2-affected genes exhibit no common transcriptional control features, and a considerable proportion of temporarily altered genes encodes proteins involved in chromatin remodeling and modification, including chromatin assembly, (anti-)silencing and histone (de- )acetylation. In addition, various metabolic pathway and nutritional supply genes are affected. Our data are compatible with the idea that CK2 acts at different levels of cellular organization and that CK2 has a global role in transcription- related chromatin remodeling
    Type of Publication: Journal article published
    PubMed ID: 12640040
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  • 5
    Keywords: CELLS ; EXPRESSION ; Germany ; INHIBITION ; KINASE ; PATHWAY ; GENE ; GENE-EXPRESSION ; GENES ; PROTEIN ; PROTEINS ; SACCHAROMYCES-CEREVISIAE ; transcription ; COMPLEX ; RESPONSES ; COMPLEXES ; DNA ; MECHANISM ; TRANSCRIPTION FACTOR ; mechanisms ; cell cycle ; CELL-CYCLE ; CYCLE ; fibroblasts ; PHOSPHORYLATION ; PROTEIN-KINASE ; ASSOCIATION ; CHROMATIN ; chromatin remodeling ; gene expression ; TRANSCRIPTIONAL ACTIVITY ; YEAST ; BUDDING YEAST ; PROMOTER ; NUMBER ; LINE ; Jun ; DEGRADATION ; ORGANIZATION ; nutrition ; REPRESSION ; expression profiling ; CYCLE CONTROL ; C-FOS EXPRESSION ; ASSOCIATIONS ; assembly ; MUTANTS ; HUMAN-CELLS ; ACTIVATOR MET4 ; CK2 knockouts ; HMGB PROTEINS ; MET genes ; PHO genes ; REGULATORY SUBUNITS ; transcription regulation
    Abstract: Protein kinase CK2 has diverse links to gene control and cell cycle. Comparative genome-wide expression profiling of CK2 mutants of the budding yeast Saccharomyces cerevisiae at cell cycle entry has revealed that a significant proportion of cell-cycle genes are affected by CK2. Here, we examine how CK2 realizes this effect. We show that the CK2 action may be directed to gene promoters causing genes with promoter homologies to respond comparably to CK2 perturbation. Examples are metabolic pathway and nutrition supply genes such as the PHO and MET regulon genes, responsible for phosphate maintenance and methionine biosynthesis, respectively. CK2 perturbation affects both regulons permanently and both via repression of a central transcription factor, but with different mechanisms: In the PHO regulon, the gene encoding the central transcription factor Pho4 is repressed and, in addition, Pho4 and/or the cyclin-dependent kinase of the regulon's control complex may be affected by CK2 phosphorylation. In the MET regulon, the repression of the central transcription factor Met4 occurs not by expression inhibition, but rather by availability tuning via a CK2-mediated phosphorylation of a degradation complex. On the other hand, the CK2 action may be directed to the chromatin regulon, thus affecting globally the expression of genes, i.e., the CK2 perturbation results either in comparable responses of genes which have no promoter homologies or in deviating responses despite promoter homologies. The effect is rather transient and concerns aside various cell cycle control genes a notable number of genes encoding chromatin remodeling and modification proteins with functions in chromatin assembly and (anti-)silencing as well as in histone (de-)acetylation, and frequently are also substrates of CK2, suggesting additional tuning at protein level. In line with these findings, we observe in human cells sequence-independent but cell-cycle-dependent CK2 associations with promoters of cell-cycle-regulated genes at periods of extensive gene expression alterations, including cell cycle entry. Our observations are compatible with the idea that the gene control by CK2 is achieved via different mechanisms and at different levels of organization and includes a global role in transcription-related chromatin remodelling and modification
    Type of Publication: Journal article published
    PubMed ID: 16335538
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  • 6
    Keywords: EXPRESSION ; Germany ; IN-VIVO ; KINASE ; VIVO ; SITE ; SITES ; GENE ; GENE-EXPRESSION ; GENES ; PROTEIN ; SACCHAROMYCES-CEREVISIAE ; transcription ; TRANSCRIPTION FACTOR ; DONOR ; IMPACT ; cell cycle ; PHOSPHORYLATION ; PROTEIN-KINASE ; METABOLITES ; DELETION ; gene expression ; protein kinase CK2 ; YEAST ; BIOSYNTHESIS ; BETA ; DEGRADATION ; REPRESSION ; expression profiling ; METHIONINE ; GENE-TRANSCRIPTION ; LEVEL ; ENZYME ; function ; ACTIVATOR MET4 ; in vivo ; AdoMet ; CASEIN KINASE-2 ; CELL-CYCLE ENTRY ; CK2 ; methionine biosynthesis ; SACCHAROMYCES ; UBIQUITIN-CONJUGATING ENZYME
    Abstract: Methionine and metabolites such as S-adenosylmethionine (AdoMet) are of vital importance for eukaryotes; AdoMet is the main donor of methyl groups and is involved in expression control of the methionine biosynthesis genes (MET genes). Genome-wide expression profiling of protein kinase CK2 deletion strains of the budding yeast Saccharomyces cerevisiae has indicated a function for CK2 in MET gene control. Deletion of the regulatory CK2 subunits leads to MET gene repression, presumably due to an impaired phosphorylation of the ubiquitin-conjugating enzyme Cdc34, which controls the central MET gene transcription factor Met4. We show that CK2 phosphorylates Cdc34 at two sites and one of these, Ser282, has a significant impact on MET gene expression in vivo, and that high AdoMet levels inhibit CK2. The data provide evidence for a control of MET gene expression by protein kinase CK2-mediated phosphorylation of Cdc34, and appear to suggest a feedback control loop in which high AdoMet-levels are limiting CK2 activity and thus MET gene expression
    Type of Publication: Journal article published
    PubMed ID: 16952051
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  • 7
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    German Medical Science; Düsseldorf, Köln
    In:  Deutscher Kongress für Orthopädie und Unfallchirurgie; 70. Jahrestagung der Deutschen Gesellschaft für Unfallchirurgie, 92. Tagung der Deutschen Gesellschaft für Orthopädie und Orthopädische Chirurgie und 47. Tagung des Berufsverbandes der Fachärzte für Orthopädie; 20061002-20061006; Berlin; DOCW.4.4.1-66 /20060928/
    Publication Date: 2007-03-09
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 8
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    German Medical Science; Düsseldorf, Köln
    In:  Deutscher Kongress für Orthopädie und Unfallchirurgie; 70. Jahrestagung der Deutschen Gesellschaft für Unfallchirurgie, 92. Tagung der Deutschen Gesellschaft für Orthopädie und Orthopädische Chirurgie und 47. Tagung des Berufsverbandes der Fachärzte für Orthopädie; 20061002-20061006; Berlin; DOCW.4.1.4-951 /20060928/
    Publication Date: 2007-03-09
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 9
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  Deutscher Kongress für Orthopädie und Unfallchirurgie; 71. Jahrestagung der Deutschen Gesellschaft für Unfallchirurgie, 93. Tagung der Deutschen Gesellschaft für Orthopädie und Orthopädische Chirurgie, 48. Tagung des Berufsverbandes der Fachärzte für Orthopädie; 20071024-20071027; Berlin; DOCW73-217 /20071009/
    Publication Date: 2007-10-10
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 10
    facet.materialart.
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  Deutscher Kongress für Orthopädie und Unfallchirurgie; 71. Jahrestagung der Deutschen Gesellschaft für Unfallchirurgie, 93. Tagung der Deutschen Gesellschaft für Orthopädie und Orthopädische Chirurgie, 48. Tagung des Berufsverbandes der Fachärzte für Orthopädie; 20071024-20071027; Berlin; DOCW24-1586 /20071009/
    Publication Date: 2007-10-10
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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