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  • 1
    Unknown
    München : Hanser
    Call number: B110:52
    Keywords: Object-oriented programming (Computer science) ; Java (Computer program language) ; Computer software / Development ; Relational databases ; Hibernate (Electronic resource)
    Notes: Translation of: Java persistence with Hibernate.
    Pages: xxvi, 703 p.
    ISBN: 978-3-446-40941-5
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    B110:52 departmental collection or stack – please contact the library
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  • 2
    Unknown
    Greenwich, CT : Manning
    Call number: B080:214
    Keywords: Object-oriented programming (Computer science) ; Java (Computer program language) ; Computer software / Development ; Relational databases ; Hibernate (Electronic resource)
    Notes: Earlier title: Hibernate in action.
    Pages: xxx, 841 p. : ill.
    Edition: Rev. ed.
    ISBN: 1932394885
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    B080:214 departmental collection or stack – please contact the library
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  • 3
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The amplification cycle consisting of NADH independent oligosaccharide dehydrogenase (ODH) and laccase has been recently reported to be highly sensitive to several catecholamines and p-aminophenol. A competitive immunoassay for 2,4-dichlorophenoxyacetic acid has been developed by combining this amplification cycle with β-galactosidase as enzyme label resulting in p-aminophenol as product. The combination of enzymatic amplification cycles with a competitive immunoassay yields a highly sensitive measurement of 2,4-dichlorophenoxyacetic acid. Using a monoclonal antibody the linear range of the assay was between 0.02 and 100 ng/l and the c50 was found at 0.2 ng/l; the detection limit was at 5 pg/l (25 fmol/l) corresponding to 5 amol.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-136X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. We have estimated the binding constant of 2,3-diphosphoglycerate (DPG) to llama and camel hemoglobin at 37°C and pH 6.7, 7.2, and 7.7 from the DPG induced change in the half saturation pressure (P 50). The values obtained were compared with the ones derived from similar measurements on human fetal and adult hemoglobin. At pH 7.2 the binding constant of DPG to llama deoxy-hemoglobin is smaller by a factor of 3 in comparison to camel hemoglobin whilst human fetal hemoglobin binds DPG six times less firmly than the human adult pigment. Camel and human adult hemoglobin have about the same affinity for DPG. 2. It is concluded that in llama hemoglobin an amino acid replacement at the DPG binding site explains the reduced phosphate affinity as compared to camel hemoglobin. In human fetal hemoglobin, in addition to an amino acid exchange, there are alterations of the anatomy of the DPG binding site which result in a poorer fit of the phosphate molecule. 3. Our data show that llama hemoglobin has a lower intrinsic oxygen affinity than camel hemoglobin so that despite the reduced DPG binding constant the oxygen affinity of llama and camel blood is very similar at equal phosphate concentration. Possibly, the amino acid exchange at the DPG binding site has compensated for the low oxygen affinity of llama hemoglobin in the course of phylogeny
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-136X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sheep haemoglobin A (Hb-A) and B (Hb-B) differ in the primary structure of the β subunits but have identical α subunits. Hb-A has a higher oxygen affinty than Hb-B which is thought to contribute to the higher resistance versus hypoxia of sheep being homozygous for Hb-A compared to sheep having Hb-B only. In order to find out if other respiratory properties of these two haemoglobins like Bohr effect, carbamate formation, reactivity towards anions or heat of reaction are likewise different we have investigated these quantities in sheep Hb-A and Hb-B. We found that the influence of protons, anions and CO2 on the oxygen affinity of Hb-A and Hb-B was very similar but observed a higher heat of reaction in Hb-A compared to Hb-B. We conclude from these results that, at least at sea level, the respiratory functions of sheep haemoglobin A and B give no indication of a selective dominance of one respiratory protein over the other.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1572-8773
    Keywords: N-acetylneuraminic acid biosynthesis ; metal ions ; selenium ; rat liver ; inhibition of enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In liver homogenate the biosynthesis ofN-acetylneuraminic acid usingN-acetylglucosamine as precursor can be followed stepwise by applying different chromatographic procedures. In this cell-free system 16 metal ions (Zn2+, Mn2+, La3+, Co2+, Cu2+, Hg2+, VO 3 − , Pb2+, Ce3+, Cd2+, Fe2+, Fe3+, Al3+, Sn2+, Cs+ and Li+) and the selenium compounds, selenium(IV) oxide and sodium selenite, have been checked with respect to their ability to influence a single or possible several steps of the biosynthesis ofN-acetylneuraminic acid. It could be shown that the following enzymes are sensitive to these metal ions (usually applied at a concentration of 1 mmoll−1):N-acetylglucosamine kinase (inhibited by Zn2+ and vandate), UDP-N-acetylglucosamine-2′-epimerase (inhibited by zn2+, Co2+, Cu2+, Hg2+, VO 3 − , Pb2+, Cd2+, Fe3+, Cs+, Li+, selenium(IV) oxide and selenite), andN-acetylmannosamine kinase (inhibited by Zn2+, Cu2+, Cd2+, and Co2+). Dose dependent measurements have shown that Zn2+, Cu2+ and selenite are more efficient inhibitors of UDP-N-acetylglucosamine-2′-epimerase than vanadate. As for theN-acetylmannosamine kinase inhibition, a decreasing inhibitory effect exists in the following order Zn2+, Cd2+, Co2+ and Cu2+. In contrast, La3+, Al3+ and Mn2+ (1 mmoll−1) did not interfere with the biosynthesis ofN-acetylneuraminic acid. Thus, the conclusion that the inhibitory effect of the metal ions investigated cannot be regarded as simply unspecific is justified.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Synthese 〈Dordrecht〉 37 (1978), S. 239-251 
    ISSN: 1573-0964
    Source: Springer Online Journal Archives 1860-2000
    Topics: Natural Sciences in General , Philosophy
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Two new enzymatic methods have been developed to quantify morphine and codeine simultaneously in a flow injection system (FIA). The first enzyme sensor for morphine or codeine is based on immobilizing morphine dehydrogenase (MDH) and salicylate hydroxylase (SHL) on top of a Clark-type oxygen electrode. Morphine or codeine oxidation by MDH leads to a consumption of oxygen by SHL via the production of NADPH. This decreases the oxygen current of the Clark electrode. Concentrations of codeine and morphine are detected between 2 and 1000 μM and between 5 and 1000 μM, respectively. The second enzyme sensor for morphine is based on laccase (LACC) and PQQ-dependent glucose dehydrogenase (GDH) immobilized at a Clark oxygen electrode. Morphine is oxidized by laccase under consumption of oxygen and regenerated by glucose dehydrogenase. Since laccase cannot oxidize codeine, this sensor is selective for morphine. Morphine is detected between 32 nM and 100 μM. Both sensors can be operated simultaneously in one flow system (FIA) giving two signals without the requirement for a separation step. This rapid and technically simple method allows discrimination between morphine and codeine in less than 1 min after injection. The sampling rate for quantitative measurements is 20 h–1. The method has been applied to the quantitative analysis of codeine or morphine in drugs.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-2013
    Keywords: Embryonic hemoglobins ; Oxygen affinity ; Bohr effect ; Phosphate effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract 1. Nucleated erythroblasts from embryonic rabbits contain two groups of tetrameric hemoglobins (Hbs): Hbs EI–III consist of embryonic α-type chains (Ξ-chains) and embryonic β-type chains (ε-chains) whilst Hbs LI–III are composed of adult α-chains and ε-chains. Structural analyses have indicated that the Ξ-chains are evolutionarily older than ε-chains. To obtain informations on possible differences in ligand binding properties associated with these embryonic chains, we have prepared Hbs EI–III and LI–III from the erythroblasts of 14-days-old embryonic rabbits and measured their oxygen affinity at various pH values and different concentrations of phosphate compounds. These data were compared with those obtained on the unfractionated embryonic hemolysate and adult rabbit hemoglobin (HbA). 2. We found that Hbs EI–III have a higher oxygen affinity than Hbs LI–III at all pH values investigated, the difference becoming larger at more acid pH. As a result, the Bohr effect is smaller in Hbs EI–III than in Hbs LI–III, Δ logP 50/Δ pH amounting to −0.25 and −0.50, respectively. In the pH range between 6.8 and 7.8 the oxygen affinities of HbA and of Hbs LI–III are alike but lower in HbA at more acid pH. These results indicate that the presence of embryonic Ξ-chains in hemoglobin tetramers raise the oxygen affinity and lower the Bohr effect of the pigment, whereas the combination of adult α-chains with embryonic ε-chains lead to hemoglobin tetramers with a very similar oxygen affinity to HbA in the physiological pH range. The cooperativity of oxygen binding was smaller both in Hbs EI–III and LI–III compared to HbA. 3. The effect of added phosphates notably of 2,3-diphosphoglycerate (2,3-DPG) on the oxygen affinity of Hbs EI–III and LI–III was very similar, i.e. the rise inP 50 produced by maximal concentrations of 2,3-DPG was not significantly different in the two types of embryonic hemoglobins. In HbA, the increase ofP 50 produced by comparable concentrations of 2,3-DPG was only slightly higher than in the embryonic hemoglobins. This shows that the embryonic ε-chains are similarly effective in binding phosphate as the adult β-chains.
    Type of Medium: Electronic Resource
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