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  • 1
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The distribution of vimentin- and desmin-positive cells in the budgerigar (Melopsittacus undulatus) dermis was investigated by means of immunohistochemical reactivity with the commercially available (Euro-Diagnostics) polyclonal antibodies. The staining pattern for vimentin in the paraffin sections was generally comparable to that in other animal species with regard to endothelial cells, vascular wall cells, muscle cells and fibroblasts. The modified Schwann cells in the inner core of the Herbst corpuscles reacted distinctly with anti-vimentin and anti-desmin. Some connective tissue cells in the superficial dermal layer, in the feather papilla and along the pulp core inside of the regenerating feathers were particularly well stained with anti-vimentin. Fibroblast-like cells of the regenerating feathers, particularly at the base of the pulp, also reacted strongly with anti-desmin. The findings were discussed with regard to references.
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  • 2
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Testes samples of 52 brown hares (Lepus europaeus L.), sacrificed between July and January, were subjected to immunohistochemical analysis. The terminal deoxynucleotidyl transferase-mediated d'UTP nick end labelling (TUNEL) method was applied to detect apoptosis; and antibodies to proliferating cell nuclear antigen (PCNA) were used to evaluate cell proliferation in the testes. In the seminiferous epithelium, the apoptotic processes were evident from August to early November with maximal values in September. Cell death in germ cells occurs predominantly during the prophase of the first meiotic division. In July, and from mid-November onwards, only the occasional TUNEL-positive cells can be seen. The proliferation of germ cells continues during the testis regression phase. The average number of PCNA-positive cells decreases slightly from September onwards and rises again in mid-November.
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  • 3
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The immunohistochemical study of chamois (Rupicapra rupicapra L.) skin showed that a limited number of available monoclonal and polyclonal antibodies expressed reactivity with skin cell components. These included cytokeratins, vimentin, desmin, neuron-specific enolase and S-100 protein with almost the same distribution pattern as already described in the skin of humans and animals. Antibodies used for labelling skin-associated lymphoid tissues and other cells with the immuno-logic function in human skin failed to demonstrate these cells in the chamois skin with the exception of LCA and OKT6 antibodies. Epidermal Langerhans cells were reliably demonstrated only by the enzyme histochemical method for adenosine triphosphatase, while the majority of mononuclear cells in dermal infiltrates showed a strong immunoreaction with OKT6 antibody. The histologic and histochemical analysis showed that the dermal infiltrations in infested skin consisted of macro-phages, lymphocytes, granulocytes, mastocytes and fibroblasts. The chamois skin affected with sarcoptes mange showed a significant loss of cytokeratins in the epidermis and its derivatives. Particular keratinocytes showing nonspecific staining with several antibodies were also described and discussed in this paper.
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  • 4
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The position and histological structure of the testes of 33 brown hares (Lepus europaeus) were studied from July to December. From July to September, the testes were located in the scrotum; in October and November, in some animals, the testes were positioned more or less in the inguinal canal towards the abdominal cavity, and in December none of the investigated animals had testes located in the scrotum. Testes were weighed and a quantitative analysis of tissue components was performed: the diameter of the seminiferous tubules, the depth of the seminiferous epithelium, the thickness of the tunica albuginea, the thickness of the peritubular tissue and the relative proportion of seminiferous tubules were determined. The tunica albuginea and peritubular tissue were thickest in September, October and at the beginning of November. In the same months the testis weight was low, and the diameter of the seminiferous tubules, the depth of the seminiferous epithelium and the relative proportion of seminiferous tubules in the testis tissue were significantly lower than in other months. We did not find any correlation between testicular regression or testis weight reduction and the change in the position of the testes. During recrudescence of spermatogenesis in November and December the testes were located in the inguinal canal.
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  • 5
    ISSN: 1432-2013
    Keywords: PCB ; Pyralene-3000 ; Lung morphogenesis ; Morphometric analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effects of the application of commercial low chlorinated PCB Pyralene-3000 on rat lung development during late gestation were studied. In the developing lungs (17.5 to 21.5 days) the volume density (Vv) of a particular compartment and length density of potential air spaces (Lv) were determined. Significant difference (p〈0.01) in epithelium to mesenchyme ratio (Epi/Mes) occurred between the control and treated groups on day 20.5 due to an increased volume proportion of mesenchyme (Vvmes) in the treated group suggesting a small delay in maturation. Decreased volume proportion of potential airspaces (Vvair) was observed on days 18.5 and 20.5. However, no differences were found on day 21.5 in any variables studied.
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