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  • 1
    Keywords: PROTEOMICS ; molecular ; chemosensitivity ; VOLUME ; imaging ; MODELS ; MODEL ; IN-VIVO ; SERIES ; REGULATOR
    Type of Publication: Book chapter
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  • 2
    Keywords: APOPTOSIS ; CANCER ; CELLS ; EXPRESSION ; INHIBITOR ; tumor ; SYSTEM ; DEATH ; PROTEIN ; PROTEINS ; TUMORS ; LINES ; MECHANISM ; MARKER ; mechanisms ; BINDING ; CELL-LINES ; DOWN-REGULATION ; NERVOUS-SYSTEM ; resistance ; CELL-DEATH ; mass spectrometry ; chemotherapy ; LINE ; MARKERS ; vimentin ; OVEREXPRESSION ; DRUG-RESISTANCE ; cell lines ; PROTEOMICS ; neuroblastoma ; chemoresistance ; multidrug resistance ; BINDING PROTEIN ; INHIBITORS ; BINDING-PROTEIN ; CHILDHOOD ; P-GLYCOPROTEIN ; SOLID TUMORS ; PROTOCOL ; cell death ; PROGNOSTIC MARKER ; ABILITY ; POLYACRYLAMIDE-GELS ; MULTIDRUG TRANSPORTER ; pediatric tumor ; THERMORESISTANCE ; TOPOISOMERASE-II-ALPHA ; two-dimensional gelelectrophoresis resistance
    Abstract: Neuroblastoma, one of the most common pediatric solid tumors, originates from the peripheral sympathetic nervous system and is responsible for approximately 15% of all childhood cancer deaths. Among the several antineoplastic drugs used in neuroblastoma chemotherapeutic protocols, topoisomerase inhibitors (i.e., etoposide) represent the most commonly used. Several resistance mechanisms limit the clinical success of topoisomerase-targeting drugs, mainly reducing the ability of neoplastic cells to start programmed cell death when exposed to antineoplastic drugs. The aim of this study was to determine, by means of proteomics, potential markers of etoposide resistance in human neuroblastoma cell lines as well as to investigate protein levels and modifications possibly involved in the onset of resistance. The etoposide resistant clone showed overexpression of the following proteins: peroxiredoxin 1, P-galactoside soluble lectin binding protein, vimentin (three protein spots), heat shock 27 kDa protein (two protein spots) and heterogeneous nuclear ribonucleoprotein K. In addition, we also found down-regulation of dUTP pyrophosphatase. This investigation might represent a first step towards the development of novel prognostic markers of neuroblastoma chemotherapy
    Type of Publication: Journal article published
    PubMed ID: 15682461
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  • 3
    ISSN: 1432-041X
    Keywords: Xenopus laevis ; Limb ; Lens formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The capacity of amputated early and late limbs of larval Xenopus laevis to promote lens-forming transformations of corneal implants in the absence of a limb regeneration blastema has been tested by implanting outer cornea fragments from donor larvae at stage 48 (according to Nieuwkoop and Faber 1956), into limb stumps of larvae at stage 52 and 57. Blastema formation has been prevented either by covering the amputation surface with the skin or by reconnecting the amputated part to the limb stump. Results show that stage 52 non-regenerating limbs could promote lens formation from corneal implants not only when innervated but also when denervated. A similar result was observed in stage 57 limbs where blastema formation was prevented by reconnecting the amputated part to the stump. In this case, relevant tissue dedifferentiation was observed in the boundary region between the stump and the autografted part of the limb. However, stage 57 limbs, where blastema formation was prevented by covering the amputation surface with skin, could promote lens formation from the outer cornea only when innervated. In this case, no relevant dedifferentiation of the stump tissues was observed. These results indicate that blastema formation is not a prerequisite for lens-forming transformations of corneal fragments implanted into amputated hindlimbs of larval X. laevis and that lens formation can be promoted by factors delivered by the nerve fibres or produced by populations of undifferentiated or dedifferentiated limb cells.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1420-9071
    Keywords: Transdifferentiation ; dorsal iris ; larvalXenopus ; amputated limb
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fragments of larvalXenopus laevis iris, autoplastically implanted into the stump of the amputated hindlimb, transdifferentiated into neural retina. However, when such iris fragments were implanted into the caudal fin, no transdifferentiative process was observed.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-041X
    Keywords: Xenopus laevis ; Thyroxine ; Propyl-thiouracil ; Limb renegeration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Regeneration in hindlimbs of Xenopus laevis larvae which were amputated at stage 53 and 55 through the tarsalia region is promoted by thyroxine (T4), while propyl-thiouracil (PTU) inhibits regeneration when compared to controls. In this paper, by in vivo and in vitro experiments, we demonstrate that the promoting effect of T4 on the regenerative processes of larval X. laevis hindlimbs is a direct effect of this hormone on the blastemal cells. By contrast, the inhibitory effect of PTU on the regenerative process is not due to a direct effect on blastemal cells or to a general toxic effect on the treated larvae, but is related to hypothyroidism induced by the drug. We find that: (i) an increase in blastemal cell proliferation is observed not only in blastemata of T4-treated larvae, but also in blastemata cultured in vitro in a medium supplemented with T4; (ii) the renegerative process is accelerated not only in larvae reared in T4 but also in larvae submitted to a combined treatment of T4 and PTU; (iii) inhibition of cell proliferation is observed in blastemata of PTU-reared larvae but not in blastemata cultured in vitro in a medium supplemented with PTU. Experiments on thyroidless larvae (which were submitted to transplantation of hindlimbs from larvae at stages 53 and 55 followed by amputation of their own right hindlimb and the transplanted limbs) have shown that without thyroid hormone the regenerative process is arrested at cone stage and the promoting effect of T4 treatment is dependent on limb stage and amputation level.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-041X
    Keywords: Xenopus laevis ; Limb regeneration ; Reamputation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Regenerated hindlimbs of larval Xenopus laevis were reamputated at critical larval stages and levels, viz when amputation of the control limb at the same larval stage and level is followed by reduced regeneration. Reamputations were performed at the level of (1) the original plane of amputation, (2) the early regenerate (cone/palette stage), (3) the late regenerate (digit stage). Reamputation increased both the percentage rate of regeneration and the morphological complexity of the regenerates in all experimental series. Cell counts in lateral motor columns and spinal ganglia innervating the hindlimb, together with histological observations and mitotic index and labelling index determinations in reamputated and control limbs showed that improved regeneration in the reamputated limb was related to an increase in undifferentiated and proliferating cells in the stump. We did not find any evidence suggesting that renewed regeneration in reamputated anuran limbs results from an increase in innervation, as has previously been hypothesized. We support our conclusions by demonstrating an improvement in regenerationen in the reamputated and denervated hindlimbs.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-041X
    Keywords: Xenopus laevis ; DNA synthesis ; Mitosis ; Limb regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Xenopus laevis larvae at stage 52–53 (according to Nieuwkoop and Faber 1956) were subjected to amputation of both limbs at the thigh level as well as to repeated denervations of the right limb. Results obtained in larvae sacrificed during wound healing (1 after amputation), blastema formation (3 days) and blastema growth (5 and 7 days) showed that denervated right limbs have undergone the same histological modifications observed in innervated left limbs and have formed a regeneration blastema consisting of mesenchymal cells with a pattern of DNA synthesis and mitosis very similar to that in presence of nerves. Also, the patterns of cellular density in regenerating right and left limbs were very similar. On the whole, the data here reported show a highly remarkable degree of nerve-independence for regeneration in hindlimbs of larval Xenopus laevis at stage 52–53 and lend some substance to the hypothesis that, in early limbs, there would exist trophic factors capable of replacing those released by nerves, promoting DNA synthesis and mitosis in blastemal cells.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-041X
    Keywords: Xenopus laevis ; thyroxine ; propyl-thiouracil ; limb regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Xenopus laevis larvae at stage 53 and 55 (according to Nieuwkoop and Faber 1956) were subjected to amputation of one or both hindlimbs and reared either in thyroxine (T4) 2.5 to 10 μg/l or in propyl-thiouracil (PTU) 0.01%. Results have shown that when the limb was amputated through a nearly undifferentiated region (tarsalia level, at stage 53) or through a differentiating region (tarsalia level, at stage 55), T4 accelerated the regenerative process and enhanced the mitotic and labelling indices of blastemal cells, when compared with controls. However, PTU delayed the regenerative process and lowered the mitotic and labelling indices. When the limb was amputated through an almost differentiated region (mid-thigh level, at stage 55), T4 inhibited the conic blastema formation, while PTU did not significatively influence limb regeneration. T4 did not modify the morphogenetic properties of the regenerative blastemata, which are characteristic of the developmental stage and the degree of differentiation of the limb tissues at the amputation level. On the whole, the data show that T4, besides being indirectly responsible for the decline of the limb regenerative capacity in a proximodistal direction by promoting limb differentiation, also exerts a direct effect on the regenerative process.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-198X
    Keywords: Key words: Preterm infant ; Hypertension ; Dehydration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Three preterm infants presented with both severe or moderate arterial hypertension and dehydration due to increased water and sodium urinary excretion. In patient 1, water and sodium wasting were extremely severe and peaked at 575 ml/kg per day and 73 mEq/kg per day, respectively. In all infants, urinary water and sodium excretion dramatically decreased when hypertension resolved. The overall clinical data suggest a pressure natriuresis phenomenon.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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