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  • 1
    ISSN: 0167-4838
    Keywords: (Pig kidney) ; Dipeptidyl peptidase IV ; Dipeptidyl-peptide hydrolase ; Proline specific endopeptidase ; Substrate specificity
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2307
    Keywords: Cystadenolymphoma ; Salivary gland ; Histogenesis ; Cytokeratin ; Vimentin ; Glial fibrillary protein (GFP) ; Co-expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The distribution of selective cytokeratin polypeptides, vimentin, and glial fibrillary protein (GFP) in 5 human cystadenolymphomas of the parotid gland was compared with normal human parotid (n=5) and submandibular (n=4) glands using a panel of monoclonal antibodies against diverse and selective cytokeratin polypeptides, vimentin and glial fibrillary protein (GFP). A biotin-streptavidin method was used on cryostat sections. The immunocytochemical finding of identical cytokeratin polypeptides Nos. 7, 8, 18 and 19 and basal cells selectively labeled by the monoclonal antibody KS 8.58, in both the epithelial part of the cystadenolymphomas and in the duct epithelium of the parotid gland, confirms the hypothesis that the epithelial compartment of cystadenolymphomas is derived from the duct system. The triple expression of cytokeratin, vimentin and GFP in myoepithelial cells of the parotid gland is discussed.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1433-044X
    Keywords: Key words ACL rupture • ACL reconstruction • Knee proprioception • Restitution of Proprioception ; Schlüsselwörter Vordere Kreuzbandruptur • VKB-Rekonstruktion • Kniegelenkpropriozeption • Propriozeptionsrestitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Kniegelenkspropriozeption wurde an 20 gesunden Probanden, an 10 Patienten mit akuter vorderer Knieinstabilität und an 20 Patienten mit chronischer vorderer Instabilität anhand einer prospektiven Longitudinalstudie bestimmt. Die Messung erfolgte unter Verwendung des Winkelreproduktionstests bei einer konstanten Winkelgeschwindigkeit von 0,5 °/s. Bei den gesunden Probanden zeigte sich keine Abhängigkeit von Körperseitendominanz und Geschlecht. Die höchsten Propriozeptionswerte wurden im endgradigen Extensionsbereich ermittelt. Die Ruptur des vorderen Kreuzbandes führte in der Akutphase zur ausgeprägten Propriozeptionsstörung, welche sich bei chronischer vorderer Instabilität nach mehrmonatiger Rehabilitation zwar signifikant verbesserte, jedoch nicht wiederhergestellt werden konnte. 3 Monate postoperativ kam es in sämtlichen Bewegungsintervallen zu einer minimalen Propriozeptionsverschlechterung, während nach 6 Monaten in Extensionsnähe und nahe 90 ° Flexion eine komplette Propriozeptionsrestitution ermittelt wurde. Im mittleren Bewegungsintervall persistierte das Propriozeptionsdefizit. Eine Überlegenheit eines Operationsverfahrens (arthroskopisch vs. Miniarthrotomie) hinsichtlich der Propriozeption zeigte sich zu keinem Zeitpunkt. Daneben wurden eine hohe Korrelation zwischen Propriozeption und subjektiver Zufriedenheit festgestellt, was die große klinische Relevanz der Propriozeption unterstreicht.
    Notes: Summary Knee proprioception was assessed in 20 healthy volunteers, 10 patients with acute anterior instability and in 20 patients with chronic anterior instability preoperatively as well as after 3 and 6 months postoperatively. To determine proprioception, an angle reproduction test was performed. There were no differences between the right and the left knee joint or between men and women. Best proprioception was measured near full extension. The acute trauma causes massive damage of the proprioception, which could be improved significantely by rehabilitation. However, rehabilitation could not restore proprioception. 3 months postoperatively, there was a slight decrease of proprioception as compared to the preoperative values, but 6 months after reconstruction, restoration of proprioception was documented near full extension and full flexion. In the mid range position, the proprioception could not be restored. There was no difference between open and arthroscopic techniques. The highest correlation was found between proprioception and patient's satisfaction.
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  • 4
    ISSN: 1434-4726
    Keywords: Laryngeal neoplasms ; Mixed salivary gland tumor ; Pleomorphic adenoma surgery ; Pleomorphic adenoma pathology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Tumors arising from minor salivary glands are extremely rare neoplasms in the larynx. Of the few reports of pleomorphic adenomas in this site , most have subglottic locations while only one case has involved the true vocal cord and seven cases have had supraglottic locations. We present a case of benign mixed tumor located in the posterior commissure which, to our knowledge, is the first reported in the world literature.
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  • 5
    ISSN: 1432-2102
    Keywords: Schlüsselwörter Sonographie ; Kopf-Hals-Tumoren ; Staging ; Treffsicherheit ; Key words Ultrasound ; Head and neck tumors ; Staging ; Accuracy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The radiologic imaging methods play an important role in the precise staging as basic requirement for an effective concept of tumor therapy. The accuracy of ultrasound in the primary staging according to the TNM-classification (UICC) was therefore prospectively investigated in 260 patients with head and neck tumors of the clinical stages T1N0 to T4N3. The clinically (C1) and sonographically (C2) evaluated pretherapeutic stages were compared to the postoperative histopathologic tumor classifications. The clinical staging was correct in 75.0 %, high in 7.7 %, low in 17.3 %, the N-stages were correct in 59.2 %, high in 17.7 %, low in 23.1 %. The sonographic staging was apparently superior with the T-classifications correct in 92.3 %, high in 7.7 %, low 0.0 %. The N-stages were correct in 89.6 %, high in 9.2 %, low in 1.2 %. The accuracy of combined TN classification rose from clinical 46.5 % to 84.6 % by sonography. Accompanying inflammations, foregoing biopsies and tooth extractions were the main reasons for incorrect staging. Therefore, the thorough sonographic investigation performed after the clinical examination and before invasive procedures, due to little patient discomfort, good availability and high accuracy, is an excellent sectional imaging method for staging, therapy-planning and follow up of tumors of the head and neck especially of the orofacial regions.
    Notes: Zusammenfassung Für eine genaue Stadienzuordnung als Grundvoraussetzung eines effektiven Tumortherapiekonzeptes spielen die bildgebenden radiologischen Verfahren eine entscheidende Rolle. Daher wurde die Treffsicherheit der Sonographie im TNM-Primärstaging gem. UICC bei 260 Patienten mit Kopf-Hals-Tumoren der klinischen Stadien T1N0 bis T4N3 prospektiv untersucht. Die prätherapeutischen klinischen (C1) und sonographischen (C2) Stadien wurden mit den postoperativen histopathologischen Tumorformeln verglichen. Klinisch war das T-Stadium korrekt in 75,0 %, zu hoch 7,7 %, zu tief 17,3 %, das N-Stadium korrekt in 59,2 %, zu hoch 17,7 %, zu tief 23,1 %. Deutlich überlegen die Sonographie mit T korrekt in 92,3 %, zu hoch 7,7 %, zu tief 0,0 %, N korrekt in 89,6 %, zu hoch 9,2 %, zu tief 1,2 %. Die Treffsicherheit stieg von 46,5 % für das klinische auf 84,6 % für das sonographische kombinierte TN-Staging. Begleitentzündungen, voraufgegangene Biopsien oder Zahnextraktionen waren Hauptursachen für Fehlklassifikationen. Somit stellt die Sonographie, durchgeführt im Anschluß an die klinische Untersuchung und vor invasiven Maßnahmen bei geringer Belastung, guter Verfügbarkeit und hoher Aussagefähigkeit ein hervorragendes Schnittbildverfahren zu Staging, Therapieplanung und Verlaufskontrolle von Kopf-Hals-Tumoren, insbesondere der orofazialen Regionen, dar.
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  • 6
    ISSN: 1432-0878
    Keywords: Ulex europaeus-lectin I ; Immunocryoultramicrotomy ; Protein A-gold technique ; Parotid gland ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Twenty non-neoplastic parotid glands (removed during neck dissection for regional tumours) were examined for cellular and subcellular binding sites of Ulex europaeus-lectin I (UEA-I), a lectin reported to be specific for α-L-fucose. For light microscopy, an extended peroxidase-antiperoxidase method was applied; for the evaluation of the subcellular localization of bound lectin, three of these glands were examined following immunocryoultramicrotomy and staining by the protein A-gold technique. In addition to the known cytoplasmic affinity of UEA-I for capillary endothelium, acinar cells bound the lectin within the cytoplasmic compartment; the number and distribution of stained acinar cells varied among individuals. Furthermore, cytomembrane-bound labelling that occurred most markedly at the luminar surface was observed in striated-duct epithelium. Using the electron microscope, protein A-gold particles were seen in zymogen granules and in Golgi cisternae of serous acinar cells; primary saliva secreted in the lumina exhibited strong labelling; serous acinar cells had binding sites on their cell membranes, striated-duct epithelium had binding sites on its surface membrane and in the vicinity of apical vesicles. Our results show that UEA-I is a useful tool for the study of the structure and functional states of the parotid gland epithelium and its associated pathological alterations.
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