Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Keywords: CELLS ; AGENTS ; human ; THERAPY ; CLASSIFICATION ; DIAGNOSIS ; SYSTEM ; TOOL ; DIFFERENTIATION ; MOLECULES ; TISSUE ; ANTIGEN ; ANTIGENS ; BIOLOGY ; MOLECULE ; antibodies ; antibody ; GLYCOPROTEIN ; SURFACE ; STRATEGIES ; pathology ; NOMENCLATURE ; AGENT ; RE ; interaction ; IMMUNE-SYSTEM ; cell differentiation
    Abstract: The immune system works through leukocytes interacting with each other, with other cells, with tissue matrices, with infectious agents, and with other antigens. These interactions are mediated by cell-surface glycoproteins and glycolipids. Antibodies against these leukocyte molecules have provided powerful tools for analysis of their structure, function, and distribution. Antibodies have been used widely in hematology, immunology, and pathology, and in research, diagnosis, and therapy. The associated CD nomenclature is commonly used when referring to leukocyte surface molecules and antibodies against them. It provides an essential classification for diagnostic and therapeutic purposes. The most recent (8th) Workshop and Conference on Human Leukocyte Differentiation Antigens (HLDA), held in Adelaide, Australia, in December 2004, allocated 95 new CD designations and made radical changes to its aims and future operational strategy in order to maintain its relevance to modern human biology and clinical practice
    Type of Publication: Journal article published
    PubMed ID: 16020511
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Interleukin (IL) -8 is a neutrophil chemoattractant cytokine with proinflammatory and growth-promoting activities, which is involved in the pathogenesis of several inflammatory diseases. It is found in high amounts in lesional biopsies of pustular diseases such as psoriasis and palmoplantar pustulosis. We report a 50-year-old woman with a 10-year history of erythroderma with disseminated pustulosis. Skin biopsies showed an epidermotropic infiltrate composed of atypical CD4+ CD8+ lymphocytes with numerous admixed neutrophils. Peripheral blood flow cytometric analysis revealed a major clonal subset of CD3+ CD4+ CD8+ T-cell receptor Vβ22+ atypical lymphocytes. Bone marrow biopsy, lymph node biopsy and computed thoracoabdominal tomography were normal. Serologies for human T-cell lymphotropic virus type I and human immunodeficiency virus were negative. Our patient’s status deteriorated despite topical (nitrogen mustard, psoralen plus ultraviolet A) and systemic (interferon, methotrexate, multiagent chemotherapy) treatments, and she finally died. We showed that our patient’s peripheral blood lymphocytes (PBL) spontaneously produced high amounts of IL-8. In contrast, PBL of patients with classical Sézary syndrome produced lower amounts of IL-8. The production of IL-8 by tumour T cells could explain this unusual clinical and histopathological presentation of cutaneous T-cell lymphoma as disseminated pustulosis.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background  We have previously established tumour T-cell lines, both from the skin and from the blood of patients with a cutaneous T-cell lymphoma (CTCL). In one patient, the tumour cells and the derived cell lines had a CD3+ CD4+ CD8– phenotype and a trisomy of chromosome 7. They expressed three T-cell receptor (TCR) β-chain transcripts, but only one was productively rearranged and expressed at the cell membrane.Objectives  In the present study, we tried to isolate a fast-growing new tumour T-cell line from the same patient.Patients/methods  We performed direct cell cloning of the skin tumour lymphocyte population, which led to the isolation of an interleukin-2-dependent highly proliferative T-cell subclone, named Cou-L3, with a CD3+ TCR-Vβ13+ CD4– CD8αα+ phenotype.Results  We demonstrated that Cou-L3 was identical to the original clonal tumour CD3+ Vβ13+ CD4+ CD8– cells, as it expressed the same rearranged TCR-Vβ13 chain. We further studied the functional activity of these CD8αα+ Vβ13+ Cou-L3 cells. We found that these cells exhibited CD3-redirected cytotoxic activity.Conclusions  An immunophenotypic shift, with a change from a CD4+ to a CD8+ phenotype, has been already reported in association with disease progression in CTCL. However, in these cases, there has been no demonstration that the phenotypic change involved the same T-cell clone. The present study is the first report of the phenotypic heterogeneity of the tumour clonal cell population in CTCL.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 1420-9071
    Keywords: Key words. Semaphorin; activation molecule; phosphatase; kinase.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. CD100 was originally described as an activation molecule on the surface of human T lymphocytes. Its triggering through distinct epitopes leads to different signals of costimulation with phorbol myristate acetate (PMA) or with CD3 and CD2. Interestingly, CD100 was shown to associate with different partner molecules in T cells. First, CD100 can associate with CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction this association is physical and has functional consequences for both partners. Second, CD100 interacts in its cytoplasmic domain with a Ser/Thr kinase for which it represents a preferential substrate. Recently, CD100 was identified as a member of the semaphorin gene family. This family comprises approximately 20 structurally related proteins. The first semaphorins were identified in the developing nervous system. Function has been shown for only some of them and involves repulsion during growth cone guidance. Since CD100 was the first semaphorin identified in the immune system, this raises the possibility of the involvement of members of the semaphorin family in other physiological phenomena outside the nervous system.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Monoclonal antibody K2H recognizes a human glycoprotein complex that is not rcstricted in haematopoietic lineages but is preferentially expressed on early haematopoietic cells, T cells, and monocytes This glycoprotein complex is made of constant ini 120,000–140,000 M1, subunitnoncovalently associated at the cell surface with subunits of higher M1. ranging from 150,000 to 200,000 on different cell types. Internal labelling with [35S]methionine and pulse-chase experiments revealed that in the cell the 120,000 M, glycoprotein of this complex is also noncnvalently associated with a 100,000 Mt glycoprotein, and that both glycoproteins are independently biosynthesized. This glycoprotein complex is shown by immunoprecipitation by lectin plus antilectin antibodies and by sequential immunoprecipitation to be one one of the cell surface structures bound by phytohaemagglutinin on the surface of normal T cells.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We have studied the interaction of mitogenic lectins such as phytohaemagglutinin (PHA) and concanavalin A (Con A) with both surface molecules which, by the use of monoclonal antibodies, are known to trigger T-cell mitogenesis. Monoclonal antibodies recognizing the T-lymphocyte receptor for antigen (Ti) and/or its associated structure, CD3, activate T cells. More recently, a second pathway of activation has been described which involves the sheep erythrocyte binding glycoprotein CD2. a surface molecule distinct from Ti-CD3. Lysates from surface-iodinated T-leukaemia cell lines were treated with lectin and affinity purified anti-lectin antibodies coupled to protein A-Sepharose. We have shown that eluatcs from Con A/anti-Con A or PHA/anti-PHA immunoprecipitates contained Ti. since;a rabbit anti-Tα serum, which recognises the native and denatured forms of the constant region of the α chain, immuno-precipitated Ti from these eluates. Furthermore, Ti immunoprecipitalcd bv anti-Tα serum from lysates of surface iodinated E lymphocvtes was binding to PHA after elution from the immunoprecipitate. When the purified Ti molecule was reduced and alkylated, allowing the permanent dissociation of its α and β subunits, PHA interacted with both chains, whereas anti-Tα serum immunoprecipitalcd the α chain onlv. Altogether, these results demonstrate that PHA interacts with both chains of the T cell receptor for antigen on human peripheral T lymphocytes. With the HPB-ALL tumour line, a similar approach showed that both α and β chains of Ti bind to Con A and Ulex europtaeus- 1 but not Helix pomatia. Affinity chomatographv on immobilized lectins and immunoprecipitation with lectin/anti-leelin antibodies were employed to test whether CD2 binds to PHA and Con A. The results show that CD2 from human peripheral T lymphoevtes binds both lectins but with a lower affinity for PHA than Con A.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Anti-D47 is a monoclonal antibody reacting with a surface antigen of human cortical thymocytes (different from the T6 antigen). It was prepared by immunization of mice with human thymocytes. The known cross-reactivity of monoclonal antibodies prepared against thymic cells with skin components prompted us to test anti-D47 on human skin as well as animal tissues. No labelling was observed on animal tissues. On human skin, anti-D47 was found to react with the secretory portion of eccrine sweat glands (ESG), i. e. with a cytoplasmic antigen of the secretory cells lining the deep portion of ESG glomeruli. No labelling was observed on apocrine sweat glands or on the excretory portion of ESG. Anti-D47 was also tested on histologically proven extramammary (vulvar) Paget's disease and basal cell epitheliomas: no staining of the neoplastic cells was observed. Anti-D47 appears to be an immunological marker of the secretory cells of human ESG and a new tool for the investigation of human sweat gland pathology.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...