Nature Archives 1869 - 2009
Process Engineering, Biotechnology, Nutrition Technology
[Auszug] B3(Fv)–PE38KDEL, a recombinant immunotoxin, forms inclusion bodies when produced in Escherichia coli. In renaturation experiments, nonspecific aggregation of non–native polypeptide chains, and the formation of incorrect disulfide linkages lead to inactive molecules. To prevent these ...
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