Springer Online Journal Archives 1860-2000
Summary Peritoneal exudate cells (PEC) of DBA/2 mice, after 7 days ofin vitro preculture and consisting of virtually 100 per cent macrophages, were able to support the replication of Herpes Simplex Virus type 1 strain WAL (HSV). Using a standard medium based on Dulbecco's Modified Eagle Medium (D-MEM), no virus replication was observed in freshly isolated PEC. However a medium based on RPMI1640 consistently yielded higher virus titres in precultured PEC than the D-MEM medium, and also allowed virus replication in freshly isolated PEC. Macrophages derived from the spleens or the bone marrow, and precultured in the same way as PEC represented a highly pure population and were permissive for infection with HSV. Titres of about 106 PFU HSV were observed in PEC 48 hours after infection with 103 or 106 PFU. However, whereas a complete destruction of the cell monolayer was observed 24 hours after infection with 106 PFU, complete cytopathogenicity in PEC infected with 103 PFU required at least twice this time. In the latter situation, plaque formation was observed 24 hours after infection. PEC of different strains of mice were compared. Of these, PEC of all mice that are susceptible to HSV infectionin vivo replicated HSV to the same degree as PEC of DBA/2 mice, whereas PEC of resistant C57 BL/6 and C3H/HeJ mice produced 1000 fold lower titres of viral progeny. Whereas the number of infectious centres were equal in PEC of DBA/2 and C57 BL/6 mice, the plaques observed after infection of confluent PEC with a low MOI were considerable smaller in cells from C57 BL/6 mice. Furthermore, significantly higher titres of interferon were measured in the supernatants of HSV-infected C57BL/6 macrophages than in those of DBA/2 macrophages, and the former were made fully susceptible by thein vitro addition of an anti-interferon serum.
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