Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Publication Date: 2012-11-06
    Description: Models of unregulated nitric oxide (NO) diffusion do not consistently account for the biochemistry of NO synthase (NOS)-dependent signalling in many cell systems. For example, endothelial NOS controls blood pressure, blood flow and oxygen delivery through its effect on vascular smooth muscle tone, but the regulation of these processes is not adequately explained by simple NO diffusion from endothelium to smooth muscle. Here we report a new model for the regulation of NO signalling by demonstrating that haemoglobin (Hb) alpha (encoded by the HBA1 and HBA2 genes in humans) is expressed in human and mouse arterial endothelial cells and enriched at the myoendothelial junction, where it regulates the effects of NO on vascular reactivity. Notably, this function is unique to Hb alpha and is abrogated by its genetic depletion. Mechanistically, endothelial Hb alpha haem iron in the Fe(3+) state permits NO signalling, and this signalling is shut off when Hb alpha is reduced to the Fe(2+) state by endothelial cytochrome b5 reductase 3 (CYB5R3, also known as diaphorase 1). Genetic and pharmacological inhibition of CYB5R3 increases NO bioactivity in small arteries. These data reveal a new mechanism by which the regulation of the intracellular Hb alpha oxidation state controls NOS signalling in non-erythroid cells. This model may be relevant to haem-containing globins in a broad range of NOS-containing somatic cells.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531883/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531883/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Straub, Adam C -- Lohman, Alexander W -- Billaud, Marie -- Johnstone, Scott R -- Dwyer, Scott T -- Lee, Monica Y -- Bortz, Pamela Schoppee -- Best, Angela K -- Columbus, Linda -- Gaston, Benjamin -- Isakson, Brant E -- HL007284/HL/NHLBI NIH HHS/ -- HL059337/HL/NHLBI NIH HHS/ -- HL088554/HL/NHLBI NIH HHS/ -- HL101871/HL/NHLBI NIH HHS/ -- HL107963/HL/NHLBI NIH HHS/ -- HL112904/HL/NHLBI NIH HHS/ -- R00 HL112904/HL/NHLBI NIH HHS/ -- R01 HL088554/HL/NHLBI NIH HHS/ -- R21 HL107963/HL/NHLBI NIH HHS/ -- England -- Nature. 2012 Nov 15;491(7424):473-7. doi: 10.1038/nature11626. Epub 2012 Oct 31.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Robert M. Berne Cardiovascular Research Center, University of Virginia, Charlottesville, Virginia 22908, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23123858" target="_blank"〉PubMed〈/a〉
    Keywords: Adrenergic alpha-1 Receptor Agonists/pharmacology ; Animals ; Cells, Cultured ; Diffusion ; Endothelial Cells/drug effects/enzymology/*metabolism ; Gene Expression Profiling ; *Gene Expression Regulation/drug effects ; Hemoglobins/genetics/*metabolism ; Humans ; Iron/chemistry ; Mice ; Nitric Oxide/*metabolism ; Nitric Oxide Synthase/metabolism ; Oxidation-Reduction ; Peptide Fragments/genetics/*metabolism ; Phenylephrine/pharmacology ; *Signal Transduction
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    Publication Date: 2018-04-11
    Description: Longer and more frequent marine heatwaves over the past century Longer and more frequent marine heatwaves over the past century, Published online: 10 April 2018; doi:10.1038/s41467-018-03732-9 Marine heatwaves are climatic extremes with devastating and long-term impacts on marine ecosystems, fisheries and aquaculture. Here the authors use a range of ocean temperature observations to identify significant increases in marine heatwaves over the past century.
    Electronic ISSN: 2041-1723
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General , Physics
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Publication Date: 2015-01-09
    Description: The mechanistic target of rapamycin complex 1 (mTORC1) protein kinase is a master growth regulator that responds to multiple environmental cues. Amino acids stimulate, in a Rag-, Ragulator-, and vacuolar adenosine triphosphatase-dependent fashion, the translocation of mTORC1 to the lysosomal surface, where it interacts with its activator Rheb. Here, we identify SLC38A9, an uncharacterized protein with sequence similarity to amino acid transporters, as a lysosomal transmembrane protein that interacts with the Rag guanosine triphosphatases (GTPases) and Ragulator in an amino acid-sensitive fashion. SLC38A9 transports arginine with a high Michaelis constant, and loss of SLC38A9 represses mTORC1 activation by amino acids, particularly arginine. Overexpression of SLC38A9 or just its Ragulator-binding domain makes mTORC1 signaling insensitive to amino acid starvation but not to Rag activity. Thus, SLC38A9 functions upstream of the Rag GTPases and is an excellent candidate for being an arginine sensor for the mTORC1 pathway.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4295826/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4295826/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wang, Shuyu -- Tsun, Zhi-Yang -- Wolfson, Rachel L -- Shen, Kuang -- Wyant, Gregory A -- Plovanich, Molly E -- Yuan, Elizabeth D -- Jones, Tony D -- Chantranupong, Lynne -- Comb, William -- Wang, Tim -- Bar-Peled, Liron -- Zoncu, Roberto -- Straub, Christoph -- Kim, Choah -- Park, Jiwon -- Sabatini, Bernardo L -- Sabatini, David M -- AI47389/AI/NIAID NIH HHS/ -- F30 CA180754/CA/NCI NIH HHS/ -- F31 AG044064/AG/NIA NIH HHS/ -- F31 CA180271/CA/NCI NIH HHS/ -- R01 CA103866/CA/NCI NIH HHS/ -- R37 AI047389/AI/NIAID NIH HHS/ -- T32 GM007287/GM/NIGMS NIH HHS/ -- T32 GM007753/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2015 Jan 9;347(6218):188-94. doi: 10.1126/science.1257132. Epub 2015 Jan 7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Department of Biology, 9 Cambridge Center, Cambridge, MA 02142, USA. Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Koch Institute for Integrative Cancer Research, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. Broad Institute of Harvard and Massachusetts Institute of Technology, 7 Cambridge Center, Cambridge, MA 02142, USA. ; Harvard Medical School, 260 Longwood Avenue, Boston, MA 02115, USA. ; Department of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, 220 Longwood Avenue, Boston, MA 02115, USA. ; Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Department of Biology, 9 Cambridge Center, Cambridge, MA 02142, USA. Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Koch Institute for Integrative Cancer Research, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. Broad Institute of Harvard and Massachusetts Institute of Technology, 7 Cambridge Center, Cambridge, MA 02142, USA. sabatini@wi.mit.edu.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25567906" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Amino Acid Transport Systems/chemistry/genetics/*metabolism ; Arginine/deficiency/*metabolism ; HEK293 Cells ; Humans ; Lysosomes/*enzymology ; Molecular Sequence Data ; Monomeric GTP-Binding Proteins/*metabolism ; Multiprotein Complexes/*metabolism ; Protein Structure, Tertiary ; Signal Transduction ; TOR Serine-Threonine Kinases/*metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  Jahrestagung der Gesellschaft für Medizinische Ausbildung (GMA); 20180919-20180922; Wien; DOCWS08 /20180919/
    Publication Date: 2018-09-20
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  16. Deutscher Kongress für Versorgungsforschung (DKVF); 20171004-20171006; Berlin; DOCV208 /20170926/
    Publication Date: 2017-09-26
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Journal for Medical Education; VOL: 33; DOC24 /20160429/
    Publication Date: 2016-04-30
    Description: Aim: Interprofessional education (IPE) is a central feature of modern education in the health care professions. Despite this, empirically founded and systematically structured IPE courses are absent from many curricula. To answer the WHO's call for improved interprofessional collaboration in the health care system, a seminar was designed, implemented and evaluated. The target group consisted of students beginning nursing and medical studies (first and second semesters) and physiotherapy students (first year of training).The aim was to develop a basic IPE module focusing not only on the demands placed by academia and politics, but also the interests of the target group. This module was evaluated on the basis of the modified four-level Kirkpatrick approach.Method: Based on focus group interviews analyzed qualitatively using Mayring's content analysis, it was possible to define five learning objectives and develop four practice-oriented modules. The seminar was then implemented and evaluated using written pre- and post-seminar evaluations and group discussions.Results: Analysis confirmed the success of the IPE concept in that the seminar was positively rated by attendees not only in terms of their immediate reactions, but also attitude, knowledge and skills according to Kirkpatrick.Conclusion: In the future, it is intended to offer the IPE module on a permanent basis and assess the competencies acquired in the seminar using observation. Courses to ensure sustained learning outcomes would also be desirable.
    Description: Zielsetzung: Interprofessionelle Lehre (IPE) ist ein zentraler Bestandteil moderner Ausbildung in den Gesundheitsberufen. Trotzdem fehlen vielerorts empirisch fundierte und systematisch aufgebaute IPE-Angebote in den entsprechenden Curricula. Um der Forderung der WHO nach verbesserter interprofessioneller Zusammenarbeit im Gesundheitswesen nachzukommen, wurde ein Seminarkonzept konzipiert, implementiert und evaluiert. Zielgruppe waren Studienanfänger der Pflegewissenschaft und Humanmedizin (1. und 2. Semester) sowie Physiotherapieschüler (1. Ausbildungsjahr).Zielsetzung war, ein IPE-Basismodul zu entwickeln, das sich neben Forderungen aus der Wissenschaft und Politik auch an Interessen der Zielgruppe orientiert und selbiges auf Basis des modifizierten Vier-Ebenen Ansatzes von Kirkpatrick evaluiert. Methodik: Basierend auf Fokusgruppeninterviews, die qualitativ mit Mayrings Inhaltsanalyse ausgewertet wurden, konnten fünf Lernziele und vier praxisorientierte Module entwickelt werden. Anschließend wurde das Seminarkonzept implementiert und mit Hilfe einer schriftlichen prä-post Erhebung sowie Gruppendiskussionen evaluiert. Ergebnisse: Die Auswertung bestätigt den Erfolg des IPE-Konzepts indem das Seminar nach Selbsteinschätzung der Teilnehmenden sowohl auf Ebene der unmittelbaren Reaktionen wie auch der Einstellung, des Wissens und der Fertigkeiten nach Kirkpatrick positiv bewertet wurde. Schlussfolgerungen: Zukünftig sollte das IPE-Modul verstetigt werden und im Seminar erworbene Kompetenzen anhand von Beobachtung überprüft werden. Weiterhin wären Veranstaltungen zur Sicherung eines nachhaltigen Lernerfolgs wünschenswert.
    Keywords: Professional role ; focus groups ; education ; interdisciplinary health team ; interprofessional relations ; students ; Professionelle Rolle ; Fokusgruppen ; Ausbildung ; Interdisziplinäre Teamarbeit ; Interprofessionelle Beziehungen ; Studenten ; ddc: 610
    Language: English
    Type: article
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Zeitschrift für Medizinische Ausbildung; VOL: 32; DOC18 /20150513/
    Publication Date: 2015-05-14
    Description: Aim: Course evaluations are often conducted and analyzed well after the course has taken place. By using a digital audience response system (ARS), it is possible to collect, view and discuss feedback during or directly following a course or lecture session. This paper analyzes a student evaluation of a lecture course with ARS to determine if significant differences exist between the results of the ARS lecture evaluation and those of the online evaluation at the end of the semester. In terms of the overall evaluation, consideration is given to the level of students' prior knowledge, the presentation of the lecture material by the lecturers and the relevance of the lecture topic for students.Method: During the 2011-12 winter semester, the lecture on Pediatrics at the Freiburg Center for Pediatric and Adolescent Medicine (Zentrum für Kinder- und Jugendmedizin (ZKJ) Freiburg) was evaluated using ARS. Thirty-four lectures were evaluated by an average of 22 (range 8-44) students, who responded to four questions each time an evaluation took place.Results: On a 6-point Likert scale (1=very good to 6=deficient), the students rated their level of preparedness with a mean of 3.18, the presentation of the lecture with 2.44, and the relevance of the lecture topic with 2.19. The overall evaluation of the lecture course by means of ARS resulted in 2.31. The online evaluation conducted at the end of the semester yielded a score of 2.45. Highly significant correlations were seen between the results of the ARS for the overall evaluation, assessment of prior knowledge, lecture presentation, and the estimated relevance of the lecture topic.Conclusion: The use of ARS is suitable for immediate evaluation of lectures, in particular regarding timely feedback for the individual lecturerlecturers. In comparison with an end-of-term evaluation, ARS yielded a better assessment.
    Description: Zielsetzung: Lehrveranstaltungsevaluationen werden oft zeitverzögert zur Lehrveranstaltung durchgeführt und ausgewertet. Mit dem elektronischen Evaluationsmedium Audience Response System (ARS) kann unmittelbar während oder nach Ende einer Lehrveranstaltung Feedback eingeholt, dargestellt und diskutiert werden. In dieser Arbeit wird eine studentische ARS-Vorlesungsevaluation analysiert. Es wird überprüft, ob signifikante Unterschiede zwischen den Ergebnissen der ARS-Vorlesungsevaluation und der Online-Evaluation am Ende des Semesters bestehen. Hierbei wird der Zusammenhang des studentischen Vorwissens, der Gestaltung der Lehrveranstaltung durch die Lehrenden sowie der Relevanz des Vorlesungsthemas für die Studierenden in Bezug auf die Gesamtbewertung betrachtet. Methodik: Im Wintersemester 2011/2012 wurde die Pädiatrievorlesung am Zentrum für Kinder- und Jugendmedizin (ZKJ) Freiburg mit Hilfe eines ARS evaluiert. Es wurden 34 Vorlesungen von durchschnittlich 22 (range 8-44) Studierenden mit jeweils vier Fragen evaluiert. Ergebnisse: Auf einer 6-stufigen Likert-Skala (1=sehr gut bis 6=ungenügend) bewerteten die Studierenden ihr Vorwissen im Mittel mit 3,18, die Vorlesungsgestaltung mit 2,44 und die Relevanz des Vorlesungsthemas mit 2,19. Die Gesamtbewertung der Vorlesung mittels ARS-Evaluation ergab die Note 2,31. In der Online Evaluation am Ende des Semesters wurde die Note 2,45 ermittelt. Es zeigt sich hoch signifikante Zusammenhänge zwischen den durch den Einsatz des ARS erhobenen Ergebnissen der Gesamtbewertung, dem Vorwissen der Studierenden, der Vorlesungsgestaltung sowie der selbsteingeschätzten Relevanz des Vorlesungsthemas. Schlussfolgerung: Der Einsatz von ARS ist zur unmittelbaren Vorlesungs- Evaluation, insbesondere im Hinblick auf zeitnahe (lehr-)personenbezogene Rückmeldungen geeignet. Im Vergleich zu einer Semesterende-Evaluation zeigt sich in der ARS-Evaluation eine bessere Bewertung.
    Keywords: Audience Response System ; ARS ; Evaluation ; Pediatrics ; Lecture ; Audience Response System ; ARS ; Evaluation ; Pädiatrie ; Vorlesung ; ddc: 610
    Language: English
    Type: article
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  Jahrestagung der Gesellschaft für Medizinische Ausbildung (GMA); 20180919-20180922; Wien; DOC44.1 /20180919/
    Publication Date: 2018-09-20
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
  • 9
    Publication Date: 2018-09-20
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  Gemeinsame Jahrestagung der Gesellschaft für Medizinische Ausbildung (GMA) und des Arbeitskreises zur Weiterentwicklung der Lehre in der Zahnmedizin (AKWLZ); 20170920-20170923; Münster; DOC335 /20171124/
    Publication Date: 2017-11-24
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...