Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A Staphylococcus epidermidis plasmid conferring inducible resistance to 14-membered ring macrolides and type B streptogramins has been analysed and the DNA sequence of the gene responsible for resistance determined. A single open reading frame of 1.464kbp, preceded by a complex control region containing a promoter and two ribosomal binding sites, was identified. The deduced sequence of the 488-amino-acid protein (MsrA) revealed the presence of two ATP-binding motifs homologous to those of a family of transport-related proteins from Gram-negative bacteria and eukaryotic cells, including the P-glycoprotein responsible for multidrug resistance. In MsrA, but not these other proteins, the two potential ATP-binding domains are separated by a Q-linker of exceptional length. Q-linkers comprise a class of flexible inter-domain fusion junctions that are typically rich in glutamine and other hydrophilic amino acids and have a characteristic spacing of hydrophobic amino acids, as found in the MsrA sequence. Unlike the other transport-related proteins, which act in concert with one or more hydrophobic membrane proteins, MsrA appears to function independently when cloned in a heterologous host (Staphylococcus aureus RN4220). MsrA might, therefore, interact with and confer antibiotic specificity upon other transmembrane efflux complexes of staphylococcal cells. The active efflux of [14C]-erythromycin from cells of S. aureus RN4220 containing msrA has been demonstrated.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1432-069X
    Keywords: Cyproterone acetate ; Ethinyl estradiol ; Sebum secretion ; Sebum composition ; Linoleic acid ; Acne
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of high-dose cyproterone acetateethinyl estradiol treatment on rates of sebum secretion and on the proportions of linoleic acid (18∶2Δ9,12) and sebaleic acid (18∶2Δ5,8) in the skin surface lipids of three female acne patients was examined. Changes in rates of sebum secretion were evaluated indirectly by measuring the ratio of wax esters/(cholesterol +cholesterol esters) in the subjects' skin surface lipid. In two of the subjects, this ratio indicated a reduction of sebum secretion rates to the childhood range. Concomitantly, there was an increase in linoleic acid and a decrease in sebaleic acid in all lipid classes. In the third subject, in whom there was only a small reduction in sebum secretion rate, the proportion of linoleic acid in the cholesterol esters more than doubled, but the changes in the other lipid classes were small or nonexistent. The results indicate that the proportions of linoleic acid and sebaleic acid in sebum are influenced by sebum secretion rates.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The aetiological role of Malassezia furfur in various dermatoses is controversial. The role of the three serovars of M. furfur in Malassezia-associated diseases has not been investigated. This study measured population densities of M. furfur serovars A. Band C. propionibacteria and Micrococcaceae on the chest, back, forehead, left and right cheeks of 10 patients with pityriasis versicolor, and 10 age- and sexmatched controls: and 10 patients with seborrhoeic dermatitis, and 10 age- and sex-matched controls. The population densities of M. furfur. propionibacteria and Micrococcaceae did not vary at a given site between patients and the corresponding control subjects. Malassezia furfu serovar A was found to be the predominant isolate on the chest and back of all four groups. but there was no difference in the distribution of serovars on the forehead and cheeks. No serovar was specifically associated with lesional skin in either disease. Thus, this data indicated that there was no difference in either the total population density of M. furfur or the distribution of serovars on lesional skin compared with control skin in either pityriasis versicolor or seborrhoeic dermatitis.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Attempts were made to produce antisera to purified preparations of Propionibacterium acnes lipase, hyaluronate lyase and acid phosphatase in rabbits. Antiserum to lipase (neutralizing titre 1:32) was produced using conventional methods. Lipase (30 μg) in Freund's complete adjuvant (FCA) was injected into multiple sites thrice at weekly intervals. Antibody levels were boosted by i.v. injections of 30 μg in saline at 2-weekly intervals for 2 months. Such regimes failed to raise antibodies to hyaluronate lyase and acid phosphatase.In order to produce antiserum to hyaluronate lyase, rabbit mononuclear cells were incubated with 45 μg of hyaluronate lyase in vitro (1 h at 37·C) prior to injection subcutaneously above the rabbit ankle joint. Simultaneous injections of hyaluronate lyase in FCA (45 μg) were administered into the footpad and thigh. Three treatments at weekly intervals produced an antiserum with a neutralizing titre of 1:256. A similar regime failed to raise antibodies to acid phosphatase.Antibodies to P. acnes lipase and hyaluronate lyase also neutralize the enzymes produced by P. granulosum. The neutralization curves suggest that the enzymes produced by both species are related antigenically but not identical.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Bacteria were sampled using a ‘scrub’ technique from the skin surface of the faces of forty-nine female subjects aged 18–21 years. The sebum excretion rate was determined by a gravimetric method and the level of free fatty acids by titration. The production rate of free fatty acids was calculated from the product of the concentration of free fatty acids in the sebum and the sebum excretion rate.The date were analysed using Kendall's rank correlation method. Positive correlations existed between the number of Micrococcaceae and the skin propionibacteria (P 〈 0.001) and between both groups of organisms and the production rate of free fatty acids (P 〈 0.001). There was no significant correlation between the size of the bacterial population and the sebum excretion rate. The results support the view that free fatty acids are produced as a result of bacterial action, that the Micrococcaceae and skin propionibacteria do not compete to the detriment of their respective populations, and that the size of the bacterial population is not dependent upon the sebum excretion rate.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Vitamin K and its analogues are frequently used in treatment of the hypoprothrombinaemia found in disease of the liver, biliary tract and small intestine. Most cases of cutaneous toxicity to vitamin K have been described in the French literature, but only two cases from Britain. This paper reports six patients with chronic liver disease who developed cutaneous reactions around the site of injection of vitamin K, and the results of investigations to further understanding of the pathogenesis of the rash.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Patients with varying degrees of acne, acne-free adult controls and samples of cord blood were investigated for cell mediated immunity to P. acnes using a leukocyte migration inhibition test. Despite the fact that the mean migration index tended to decrease with acne severity, only the patients with severe acne showed cell-mediated immunity. It is suggested that when cellular immunity arises it is a late event which may contribute to inflammation but is probably not a factor in its initiation.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary.— The sebum excretion rate and skin surface lipid composition were determined in 193 male and female control subjects aged between 4 and 60 years. The sebum excretion rate, squalene and wax ester content of surface lipid were maximal in males aed 26–40. The percentages of surface lipid wax esters were greater in males than females at all ages, but the prepubertal peak of wax esters in males may be due to an epidermal contribution from sterol esters. There was no significant age or sex variation in the sum of the percentages of triglycerides and free fatty acids, but the degree of hydrolysis varied considerably with age. The free fatty acid content was greatest in the first decade in both sexes, when the sebum excretion rate is at the lowest levels. Cholesterol content of surface lipid rose to maximal levels at puberty in both sexes.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 86 (1972), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 86 (1972), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary.— Three case of unilateral idiopathic hyperhidrosis are described. Two of the lesions were congenital; the thrid occurred in later life. Quantitative sweat collections demonstrated that the sweat glands in the lesion were hyperresponsive to those stimuli, which normally evoked sweating in the skin sites where the lesions occurred. Hyper-responsiveness of the sweat glands to cholinergic stimuli was also demonstrated, and histology revealed greatly enalrged glands.These results suggest that the hyperhidrosis is due to Increased glandular mass, although sweat gland hypersensitivity could notberuled out as an additional contributing factor to the hyperhidrosis.Local anticholinergic therapy was found to besuccessful in the treatment of all 3 patients.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...