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  • 1
    ISSN: 1432-2048
    Keywords: Key words: Adhesion (algal) ; Biofouling ; Cell coat ; Enteromorpha (adhesion) ; Glycoprotein ; Secretion (glycoprotein)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Zoospores of Enteromorpha compressa (L.) Grev. secrete an adhesive cell coat which is involved in their attachment to various substrata. Two monoclonal antibodies (mAbs), designated Ent 1 and Ent 6, were raised against settled zoospores displaying secreted adhesive. Both antibodies labelled specifically the anterior region of the cell containing putative adhesive vesicles. During settlement the antigens recognised by both mAbs were secreted but whereas Ent 6 recognised a fibrillar material released within a few minutes of settlement, Ent 1 recognised components which were associated predominantly with the developing cell wall at later time points. Both mAbs also labelled a Golgi-rich region of settled spores, suggesting that these antigens are also synthesised after settlement. Both mAbs labelled the cell walls of vegetative tissue. Competitive enzyme-linked immunosorbent assay indicated that the two antibodies recognise separate, but overlapping epitopes. In spore settlement assays the Ent 6 immunoglobulin strongly reduced initial adhesion at low concentration whereas the inhibitory effects of Ent 1 occurred at later time points. On analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, (SDS-PAGE) both MAbs recognised a major buffer- and SDS-soluble, polydisperse 110-kDa antigen. The 110-kDa component was present in extracts of zoospores and sporulating tissue, but absent, in soluble form, from vegetative tissue. Deglycosylation of zoospore extract with anhydrous HF and peptide N-glycosidase digestion, showed that the major 110-kDa antigen is an N-linked glycan, and that the epitope is borne by the protein component. Time-course experiments showed that the Ent 6 antigen became progressively insoluble after zoospore attachment. Taken together, the data indicate that the two antibodies recognise separate but closely related antigens which have distinctive roles in adhesion and cell wall development.
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 268 (1977), S. 626-627 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fucoid eggs secrete a polysaccharide cell wall immediately after fertilisation10*11. Accordingly we have used the fluorescent brightener Calcofluor white ST (Cyanamid) to stain zygotes permitting a rapid and direct assessment of the level of fertilisation by ultraviolet microfluorescence ...
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  • 3
    ISSN: 0045-6039
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-6776
    Keywords: biofilm ; Citrobacter ; metal-bioremediation ; microelectrode ; pH gradient
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Monospecies Citrobacter sp. biofilms were grown in a laminar flow cell using a carbon-limiting medium. Microelectrode measurements showed no change in pH between the bulk fluid and biofilm when the flow cell was supplied with the carbon-limiting medium under static or flowing conditions. When the biofilm was supplied with a phosphate-limiting medium the biofilm became more acidic than the bulk fluid and developed a gradient within. The implications for metals-bioremediation processes are discussed.
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  • 6
    ISSN: 1615-5947
    Keywords: Balloon catheter ; mechanical injury ; hypercholesterolemia ; intimal hyperplasia ; lesion ; atherosclerosis ; revascularization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In an attempt to clarify data obtained from animal models of intimal hyperplasia, we used New Zealand white rabbits, a standardized balloon catheter injury model, and a 0.25% cholesterol supplemented diet. The effects of mechanical injury and hyper-cholesterolemia separately and combined were determined at the carotid and iliac positions at 12 weeks. En-face planimetry of lesioned intima and measurement of transverse intima-to-media thickness were taken as indices of intimal hyperplasia. No animals received antiplatelet agents or postoperative anticoagulation and all vessels remained patent. Neither procedure alone resulted in statistically significant lesion increase. However, combinations of injury and cholesterol resulted in statistically significant and synergistic lesion enhancement. The quantitative data, coupled with distinctive features noted on scanning electron microscopy and transmission electron microscopy, showed separate and synergistic effects of mechanical injury and cholesterol diet on intimal lesions in this model. Additionally, these effects must be considered in evaluation of animal models of intimal hyperplasia and atherosclerosis. Furthermore, this may help dissect mechanisms of failed revascularizations.
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  • 7
    ISSN: 1573-7438
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Un antigène préparé en Australie et des sérums anti récoltés sur du bétail de Mozambique naturellement infecté de babesiose ont été utilisés pour comparer des souches deBabesia bovis des deux pays. La réaction des antisérums du Mozambique vis-à-vis de l'antigène australien a été semblable à celle obtenue avec des antisérums importés, ce qui suggère qu'une étroite parenté sérologique existe entre lesB. bovis des deux pays. L'incidence pratique de cette découverte réside dans le fait que le vaccin australien pourrait protéger le bétail introduit en Afrique du Sud à partir de régions indemnes deB. bovis.
    Abstract: Resumen Se llevaron a Mozambique, con fines investigativos, un antígeno preparado en Australia y antisueros de bovinos que habían sufrido babesiosis en forma natural para efectuar la comparación serológica de cepas deBabesia bovis de los dos países. La reacción de los antisueros colectados en Mozambique contra el antígeno australiano fue similar a aquella del antisuero importado. Este resultado sugiere una relación serológica similar entre las cepas deB. bovis de ambos países. Esto significa también que la vacuna australiana debería proteger bovinos introducidos al Sur de Africa provenientes de áreas libres deB. bovis.
    Notes: Summary An antigen prepared in Australia and antisera collected from cattle having had field infections of babesiosis were taken to Mozambique for a serological comparison of strains ofBabesia bovis from the 2 countries. The reactivity of antisera collected in Mozambique against the Australian antigen was similar to that of the imported antisera. This suggested a close serological relationship betweenB. bovis of the 2 countries. The practical implication of this finding is that Australian vaccine should protect cattle being introduced into southern Africa fromB. bovis-free environments.
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  • 8
    ISSN: 1435-5663
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract This paper describes basic research issues and our experience in the development of an intelligent modeling environment for use with a large engineering analysis code. The intelligent environment, the Athena Aide, is a working prototype system that assists analysts in the preparation of models for the ATHENA thermal-hydraulics code. The paper includes the background and basis for our research and descriptions of the analysis code and the intelligent environment, and discusses research topics addressed during the development including design goals, general and specific design issues, information management and representation issues, and user acceptance issues related to the model construction task. Our research has shown that data management is a fundamental consideration during the model construction task and an intelligent environment is a valuable assistant to the analyst in that regard. Providing modeling advice commensurate with the general nature of the analysis code was found to be extremely difficult. For modeling tasks where specific heuristic knowledge has accumulated, useful modeling advice can be provided.
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  • 9
    ISSN: 1573-5028
    Keywords: cab gene ; expression cloning ; immunoscreening ; LHCI ; photosystem I ; Pisum sativum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A monoclonal antibody (MAb UB42) is described that binds to thylakoids in pea chloroplasts, as shown by EM-immunogold labelling. The antibody recognised proteins of ca. 23–29 kDa in western blots of a pea leaf homogenate. A cDNA library was prepared from pea epidermal cells in the vector λZAP II, and immunoscreening of the library with UB42 led to the isolation of a clone, pUB42. This was sequenced and had an open reading frame of 269 codons encoding a predicted polypeptide of 28.9 kDa. The sequence showed extensive homology with three closely related polypeptides belonging to a family of chlorophyll a/b-binding proteins from the light harvesting complex of photosytem I (LHCI). Collectively, the results suggest that MAb UB42 recognises an epitope on the type II chlorophyll a/b-binding protein from LHCI and that clone pUB42 encodes this protein.
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  • 10
    ISSN: 1570-7458
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé On a suivi les voies de translocation et de pénétration de l'acide 9-oxodec-trans-2 enoïque, marqué avec du tritium et appliqué à la surface du corps d'abeilles vivantes. On a noté un transfert peu important mais défini, de la surface du thorax vers l'abdomen, avec pénétration à travers le tégument et échange du tritium avec d'autres composés. Les détails du processus par lequel la phéromone est transférée des glandes mandibulaires de la reine à l'ensemble de la colonie n'ont pas été déterminés avec certitude à l'aide de cette technique.
    Notes: Abstract Movement of 9-oxodec-2-enoic acid, a queen honeybee pheromone, from one part of a bee to another was studied using material labelled with tritium. Translocation was demonstrated, apparently partly on the surface and partly internally. Tritium exchange with tissue material caused some difficulty in precise interpretation of the results.
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