human hepatocelluar carcinoma
Springer Online Journal Archives 1860-2000
Chemistry and Pharmacology
Abstract Integrin α 5 β 1 and α 2 β 1 are the major integrin receptors in human hepatocytes. However, in human hepatocellular carcinoma cells it was found that the expression of integrin α 5 β 1 was decreased and another integrin α 6 β 1 increased. In this study, the SMMC7721 human hepatocellular carcinoma cells cotransfected or singlely transfected with integrin α 5 and/orβ 1 cDNAs were established, and designatedα 5 β 1.6-7721, α 5.3-7721, and β 1.6-7721 cell lines, respectively. Transfection with cDNAs of integrin α 5 and β 1 subunits resulted in the overexpression of each integrin and modified biological properties, including a slowed growth rate, changes in the cell cycle from 15.5% of control cells in the G2/M phase to 12.1%, 9.6% and 9.4% in α 5.3-7721, β 1.6-7721, α 5 β 1.6-7721, respectively, and a decrease in the Cell Mitosis Index from 1.6 in controls to 0.96, 0.95, and 0.72, and 34%, 28% and 52% derived from colony forming ability, respectively. Tumorigenicity was also tested in nude mice with inoculation of cells subcutaneously. Tumor masses growing in nude mice following inoculation with β 1.6-7721,and α 5 β 1.6-7721 cells weighed only 52% or 31% those of control cells. These results indicated that deletion or low expression of integrin α 5 β 1 may play an important role in the development of hepatocellular carcinoma. Therefore, induction of expression of the integrin α 5 β 1 in malignant cells could be a potential means of treating hepatocellular carcinoma.
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