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  • 1
    Keywords: Life sciences ; Cytology ; Developmental Biology ; Embryology ; Life sciences ; Cell Biology ; Embryology ; Developmental Biology ; Springer eBooks
    Description / Table of Contents: Methods For Sperm Concentration Determination -- Methods of Sperm Vitality Assessment -- The Hypo-osmotic Swelling Test for Evaluation of Sperm Membrane Integrity -- Sperm Morphology Classification: A Rational Method For Schemes Adopted By The World Health Organization -- Sperm Morphology Assessment Using Strict (Tygerberg) Criteria -- Methods For Direct And Indirect Antisperm Antibody Testing -- Manual Methods For Sperm Motility Assessment -- Computer-Aided Sperm Analysis (CASA) Of Sperm Motility And Hyperactivation -- The Hemizona Assay For Assessment Of Sperm Function -- The Sperm Penetration Assay for the Assessment of Fertilization Capacity -- Methods For The Assessment Of Sperm Capacitation And Acrosome Reaction Excluding The Sperm Penetration Assay -- Sperm DNA Fragmentation Analysis Using the TUNEL Assay -- Sperm DNA Damage Measured by Comet Assay -- Sperm Chromatin Structure Assay (SCSA℗ʼ) -- Sperm Aneuploidy Testing Using Fluorescence In Situ Hybridization (FISH) -- Flow Cytometric Methods For Sperm Assessment -- Human Y Chromosome Microdeletion Analysis By PCR Multiplex Protocols Identifying Only Clinically Relevant AZF Microdeletions -- Sperm Cryopreservation Methods -- Density Gradient Separation Of Sperm For Artificial Insemination -- Recovery, Isolation, Identification and Preparation of Spermatozoa from Human Testis -- Enhancement of Sperm Motility Using Pentoxifylline and Platelet-Activating Factor -- Intracytoplasmic Morphology Selected Sperm Injection -- Sperm Selection For ICSI Using Annexin V -- Sperm Selection for ICSI Using the Hyaluronic Acid Binding Assay -- Sperm Selection Based On Electrostatic Charge -- Sex-Sorting Sperm Using Flow Cytometry/Cell Sorting -- Assessment of Spermatogenesis Through Staging of Seminiferous Tubules -- Immunohistochemical Approaches for the Study of Spermatogenesis -- Ultrastructural Analysis of Testicular Tissue and Semen by Transmission and Scanning Electron Microscopy -- Assessment Of Oxidative Stress In Sperm And Semen -- Improved Chemiluminescence Assay for Measuring Antioxidant Capacity of Seminal Plasma -- Methods of Sperm DNA Extraction for Genetic and Epigenetic Studies -- Isolating mRNA and Small Noncoding RNAs From Human Sperm -- A Review Of Genome-Wide Approaches To Study The Genetic Basis For Spermatogenic Defects -- Methods For The Analysis Of The Sperm Proteome -- Methodology of Aniline Blue Staining Of Chromatin and the Assessment of the Associated Nuclear and Cytoplasmic Attributes℗ in Human Sperm -- Isolation of Sperm Nuclei and Nuclear Matrices from the Mouse, and Other Rodents -- Extraction and Analysis of Human Sperm Protamine 1 / Protamine 2 Ratio Using Acid Gel Electrophoresis -- Analysis of Gene-Specific and Genome-Wide Sperm DNA Methylation -- Evaluating the Localization and DNA Binding Complexity of Histones in Mature Sperm -- In Vitro Spermatogenesis Using An Organ Culture Technique -- Testicular Tissue Grafting and Male Germ Cell Transplantation -- Transgenic Modification of Spermatogonial Stem Cells Using Lentiviral Vectors -- Methods for Sperm-Mediated Gene Transfer -- Phenotypic Assessment of Male Fertility Status in Transgenic Animal Models
    Abstract: Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population.℗ Spermiogenesis and Spermatogenesis: Methods and Protocols℗ details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins.℗ Written in the successful℗ Methods in Molecular Biologý„Ø℗ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible,℗ Spermiogenesis and Spermatogenesis: Methods and Protocols℗ is℗ unique in its breadth, and will be a useful reference for clinicians and researchers alike
    Pages: XVII, 554 p. 110 illus., 68 illus. in color. : digital.
    ISBN: 9781627030380
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  • 2
    Keywords: Medicine ; Gynecology ; Endocrinology ; Reproductive Medicine ; Urology ; Medicine & Public Health ; Gynecology ; Endocrinology ; Reproductive Medicine ; Urology/Andrology ; Springer eBooks
    Pages: : digital
    ISBN: 9781441984562
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  • 3
    Keywords: Medicine ; Gynecology ; Endocrinology ; Reproductive Medicine ; Urology ; Medicine & Public Health ; Endocrinology ; Reproductive Medicine ; Urology/Andrology ; Gynecology ; Springer eBooks
    Pages: : digital
    ISBN: 9781441914361
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  • 4
    ISSN: 1573-7330
    Keywords: coculture ; cumulus tissues ; embryo morphology ; in vitro fertilization ; pregnancy rates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Purpose: This study was undertaken to evaluate simplified methods of human embryo coculture using either attached or nonattached autologous cumulus tissue. Methods: Eight hundred one zygotes were cultured for 48 hr in a prospective, randomized trial comparing culture of embryos either with intact cumulus tissue, with cumulus tissue added to the droplet of culture medium, or without any cumulus tissue. In a follow-up study, embryo quality, pregnancy rates, and implantation rates were compared in 120 consecutive patients undergoing in vitro fertilization with a coculture system using cumulus tissue compared to a cohort of 127 patients undergoing IVF immediately preceding the institution of the coculture protocol. Results: Embryo morphology was significantly improved (P 〈 0.05) following culture with attached cumulus tissue (5.61 ± 0.29) and culture with added cumulus tissue (4.72 ± 0.31) compared to that of embryos grown in culture medium without cumulus tissue (3.95 ± 0.26). The clinical pregnancy rate improved from 39.4% (50/127) to 49.2% (59/120) following institution of a system of coculture with attached cumulus tissue. Conclusions: These data indicate that a simple coculture system using autologous cumulus tissue can result in improved embryo morphology, implantation rates, and clinical pregnancy rates during in vitro fertilization. This coculture system is simple, is non-labor intensive, and eliminates many of the risks which may be present in other embryo coculture systems.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-7330
    Keywords: protamine ; chromatin ; decondensation ; capacitation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Purpose: The aim of this study was to evaluate the possible correlation of low-dose heparin-induced decondensation of sperm chromatin with sperm concentration, motility, morphology, membrane hypoosmotic response, ejaculate volume, and the ability of sperm to penetrate zona-free hamster oocytes. Methods: Twenty-two donors of known fertility and 105 patients undergoing evaluation at an andrology laboratory were evaluated by standard World Health Organization semen analysis techniques and a modified sperm penetration assay (SPA). An aliquot was also incubated for 60 min and Ham's F10 medium containing 50 USP/ml heparin. The percentage of sperm undergoing chromatin decondensation was evaluated and correlated to SPA rates and semen quality parameters. Results: No significant correlation was observed between semen parameters and decondensation rates. A nonsignificant (P = 0.11) inverse correlation (P = −0.21) wax observed between SPA rates and chromatin decondensation. Significant (P 〈 0.001) differences were observed in the decondensation rate of donors (3.7 ± 0.6), patients with normal SPA rates (7.8 ± 1.5), and patients with decreased SPA rates (21.7 ± 1.8). The decondensation rates were significantly different (P 〈 0.01) between patients with a normal SPA rate and patients with a decreased SPA rate. Conclusions: These data indicate a significant inverse relationship between the SPA rate, which has previously been shown to correlate highly with fertilization ability, and heparin-induced sperm chromatin decondensation.
    Type of Medium: Electronic Resource
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