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  • 1
    Keywords: Life sciences ; Proteins ; Life sciences ; Protein Science ; Springer eBooks
    Description / Table of Contents: Platforms for Recombinant Therapeutic Glycoprotein Production -- Uncovering Innovation Features and Emerging Technologies in Molecular Biology through Patent Analysis -- Production of Full-length Antibody by Pichia Pastoris -- Human cells as Platform to Produce Gamma-Carboxylated Proteins -- Production of Recombinant Factor VIII in Human Cell Lines -- Strategies to Suspension Serum-Free Adaptation of Mammalian Cell Lines for Recombinant Glycoprotein Production -- Production of℗ Recombinant Rabies Virus Glycoprotein by Insect Cells in℗ a Single Use Fixed-bed Bioreactor -- Cell-Free Production of Protein Biologics within 24 hours -- Demonstration-Scale High-Cell-Density Fermentation of Pichia Pastoris -- Large Scale Transient Transfection of Suspension Mammalian Cells for VLP Production -- Bioreactor-Based Production of Glycoproteins in Plant Cell Suspension Cultures -- Fed-Batch CHO Cell Culture for Lab-Scale Antibody Production -- Strategies to Develop Therapeutic N- and O-Hyperglycosylated Proteins -- Expression of Glycosylated Proteins in Bacterial System and Purification by Affinity Chromatography -- Purification Methods for Recombinant Factor VIII Expressed in Human Liver SK-Hep Cells -- Purification Method for Recombinant hG-CSF by Affinity Chromatography -- Microplate-Based Method for High-Throughput Screening (HTS) of Chromatographic Conditions Studies for Recombinant Protein Purification -- Purification and Auto Activation Method for Recombinant Coagulation Factor VII -- Preparation of Immunoliposomes by Direct Coupling of Antibodies Based on a Thioether Bond -- Polyester-Based Nanoparticles for the Encapsulation of Monoclonal Antibodies -- Polyester-Based Nanoparticles for Delivery of Therapeutic Proteins -- Quantification of Coagulation Factor VIII by Selective Reaction Monitoring
    Abstract: This volume covers a wide spectrum of techniques and approaches that are used in the upstream and downstream processing for recombinant glycoprotein production. Chapters guide the reader through state-of-art of therapeutic recombinant glycoproteins, explores the patent literature, expression systems used for glycoproteins production, methods employed in the downstream processing of different glycoproteins, and information about analytical tools and formulation strategies. Written in the highly successful℗ Methods in Molecular Biology℗ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Glycoprotein Production: Methods and Protocols℗ aims to ensure successful results in the further study of this vital field
    Pages: XI, 285 p. 51 illus., 37 illus. in color. : online resource.
    ISBN: 9781493973125
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  • 2
    Keywords: Life sciences ; Human Genetics ; Cytology ; Life sciences ; Cell Biology ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Predictive Binding Geometry of Ligands to DNA Minor Groove:℗ Isohelicity and Hydrogen-Bonding Pattern -- Using Microchip Gel Electrophoresis to Probe DNA-Drug Binding Interactions -- Identifying Different Types of Chromatin using Giemsa Staining -- Analysis of DNA Damage and Repair by Comet Fluorescence in situ Hybridization (Comet-FISH) -- Alkaline Nuclear Dispersion Assays for the Determination of DNA Damage at the Single Cell Level -- Polarization Microscopy of Extended Chromatin Fibres -- DNA Labeling in Vivo: Quantification of Epidermal Stem Cell Chromatin Content in Whole Mouse Hair Follicles Using Fiji Image Processing Software -- A Historical Overview of Bromo-Substituted DNA and Sister Chromatid Differentiation -- Image Analysis of Chromatin Remodelling -- FISH Methods in Cytogenetic Studies -- Ultrastructural and Immunofluorescent Methods for the Study of the XY Body as a Biomarker -- Atomic Force Microscopy for Analyzing Metaphase Chromosomes. Comparison of AFM Images with Fluorescence Labeling Images of Banding Patterns -- Selective Detection of Phagocytic Phase of Apoptosis in Fixed Tissue Sections -- Transport of Cationized Fluorescent Topoisomerase into Nuclei of Live Cells for DNA Damage Studies -- Visualization and Interpretation of Eukaryotic DNA Replication Intermediates in vivo by Electron Microscopy -- Combined Bi-dimensional Electrophoresis and Electron Microscopy to Study Specific Plasmid DNA Replication Intermediates in Human Cells -- Standard DNA Methylation Analysis in Mouse Epidermis: Bisulfite Sequencing, Methylation-Specific PCR, and 5-Methyl-Cytosine (5mC) Immunological Detection -- Methyl-combinǵ€”Single Molecule Analysis of DNA Methylation on Stretched DNA Fibers -- Investigating 5-Hydroxymethylcytosine (5hmC): The State of the Art -- Hydroxymethylated DNA Immunoprecipitation (hmeDIP) -- Microscale Thermophoresis for the Assessment of Nuclear Protein Binding Affinities -- Analysis of Histone Post-translational Modifications from Nucleolus-associated Chromatin by Mass Spectrometry -- Salt-Urea, Sulfopropyl-Sepharose, and Covalent Chromatography Methods for Histone Isolation and Fractionation -- Chromatin Immunoprecipitation -- Analysis of Chromatin Composition of Repetitive Sequences: the ChIP-chop Assay -- Purification of Specific Chromatin Domains from Single-Copy Gene Loci in Saccharomyces cerevisiae -- Deep Sequencing of Small Chromatin-Associated RNA ́€“ Isolation and Library Preparation -- Deep Sequencing of Small Chromatin-Associated RNA ́€“ Bioinformatic Analysis
    Abstract: In Functional Analysis of DNA and Chromatin, expert researchers in the field provide an overview of standard and more recent methods for the functional analysis of the genetic material. These include methods on DNA-dye binding modes, chromatin staining, nuclear dispersion assays, DNA labeling in vivo, sister chromatid exchanges, FISH, DNA and chromatin imaging by fluorescence, electron and atomic force microscopy, detection of apoptotic DNA, cytosine methylation and hydroxy-methylation, DNA thermophoresis, improved methods for histone analysis, chromatin immunoprecipitation, and analysis of rDNA genes and chromatin-associated RNA. Written in the highly successful Methods in Molecular Biology ℗ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative℗ and practical,℗ Functional Analysis of DNA and Chromatin seeks to aid scientists in the further study of cellular and molecular biology of the nucleus
    Pages: XV, 365 p. 74 illus., 44 illus. in color. : online resource.
    ISBN: 9781627037068
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  • 3
    Keywords: Medicine ; Radiology, Medical ; Cardiology ; Medicine & Public Health ; Cardiology ; Diagnostic Radiology ; Springer eBooks
    Pages: : digital
    ISBN: 9781849960830
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  • 4
    Keywords: Cancer Research ; Cell Biology ; Immunology ; Pharmacology ; Cancer Research ; Cell Biology ; Immunology ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: CAR-T Cells for Cancer Treatment: Current Design and Next Frontiers -- Selection of Antibody Fragments for CAR-T Cell Therapy from Phage Display Libraries -- Phase I/II Manufacture of Lentiviral Vectors under GMP in an Academic Setting -- Production of Lentivirus for the Establishment of CAR-T Cells -- Optimized Production of Lentiviral Vectors for CAR-T Cell -- Lentiviral Vectors Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells -- In vitro Transcribed (IVT)-mRNA CAR Therapy Development -- Generation of Chimeric Antigen Receptor T Cells Using Gammaretroviral Vectors -- Generation of CAR+ T Lymphocytes using the Sleeping Beauty Transposon System -- Platforms for Clinical-grade Car-T Cell Expansion -- CAR-T Cell Expansion in a Xuri Cell Expansion System W25 -- Methods and Process Optimization for Large Scale CAR T Expansion Using the G-Rex Cell Culture Platform -- Replication Competent Lentivirus Analysis of Vector Transduced T Cell Products used in Cancer Immunotherapy Clinical Trials -- Immunophenotypic Analysis of CAR-T Cells -- Approaches of T Cell Activation and Differentiation for CAR-T Cell Therapies -- Determination of Cytotoxic Potential of CAR-T Cells in Co-cultivation Assays -- Analysis of CAR-mediated Tonic Signaling -- Generation of Tumor Cells Expressing Firefly Luciferase (fLuc) to Evaluate the Effectiveness of CAR in a Murine Model -- Analysis of Antitumor Effects of CAR-T Cells in Mice with Solid Tumors
    Abstract: This volume provides comprehensive methods from expert scientists working in the Chimeric Antigen Receptor T Cell (CAR-T Cell) field. Chapters guide readers through the state-of-art of CAR-T cell technology, CAR design and vector production, CAR-T cell generation and manufacturing, CAR-T cell characterization, and quality control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chimeric Antigen Receptor T Cells: Development and Production aims to be useful in the production of CAR-T cells, especially for therapeutic purposes
    Pages: XI, 274 p. 29 illus., 25 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781071601464
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  • 5
    Unknown
    Dubuque, IA : WCB
    Call number: 04-Zell:370
    Keywords: Marine Biology ; Meer / Biologie
    Pages: xiv, 450 p. : ill.
    Edition: 2nd ed.
    ISBN: 0-697-24360-5
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    04-Zell:370 departmental collection or stack – please contact the library
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  • 6
    Keywords: Life sciences ; Microbial ecology ; Microbiology ; Soil science ; Soil conservation ; Environmental engineering ; Biotechnology ; Life sciences ; Microbial ecology ; Microbiology ; Soil Science & Conservation ; Environmental Engineering/Biotechnology ; Springer eBooks
    Abstract: This book describes selected microbial genera from the perspective of their environmentally and commercially sustainable use. By focusing on their physiology and metabolism and combining historical information with the latest developments, it presents a multidisciplinary portrait of microbial sustainability. The chapters provide readers descriptions of each genus in the form of microbial models that move us closer to the goal of sustainability; selected chapters also include worldwide market information and lists of corresponding patents
    Pages: IX, 349 p. 64 illus., 28 illus. in color. : online resource.
    ISBN: 9789811025556
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  • 7
    Publication Date: 2018-06-28
    Description: Septicemia due to Streptococcus dysgalactiae subspecies dysgalactiae in vampire bats ( Desmodus rotundus ) Septicemia due to 〈i〉Streptococcus dysgalactiae〈/i〉 subspecies 〈i〉dysgalactiae〈/i〉 in vampire bats (〈i〉Desmodus rotundus〈/i〉), Published online: 27 June 2018; doi:10.1038/s41598-018-28061-1 Septicemia due to Streptococcus dysgalactiae subspecies dysgalactiae in vampire bats ( Desmodus rotundus )
    Electronic ISSN: 2045-2322
    Topics: Natural Sciences in General
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  • 8
    Publication Date: 2018-07-20
    Description: Author Correction: Septicemia due to Streptococcus dysgalactiae subspecies dysgalactiae in vampire bats ( Desmodus rotundus ) Author Correction: Septicemia due to 〈i〉Streptococcus dysgalactiae〈/i〉 subspecies 〈i〉dysgalactiae〈/i〉 in vampire bats (〈i〉Desmodus rotundus〈/i〉), Published online: 19 July 2018; doi:10.1038/s41598-018-29407-5 Author Correction: Septicemia due to Streptococcus dysgalactiae subspecies dysgalactiae in vampire bats ( Desmodus rotundus )
    Electronic ISSN: 2045-2322
    Topics: Natural Sciences in General
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  • 9
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1438-2199
    Keywords: Amino acids ; Carbon tetrachloride ; Hydroxyproline ; Free radicals ; Collagen ; Cirrhosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Benzoyl peroxide catalytic decomposition of carbon tetrachloride in a model system produces trichloromethyl and trichloromethylperoxyl free radicals. These radicals are also produced by CCl4 bioactivation in liver and are considered to be responsible for the deleterious effects of this hepatotoxin. In this study, it is attempted to learn about how the .CCl3 and CCl3O2. tend to react with hydroxyproline in a model system. Hydroxyproline was selected because of its role in collagen metabolism. During the interaction of both radicals with hydroxyproline a total of 16 reaction products were isolated and identified by gas chromatographic-mass spectrometric analysis. All of them were hydroxyproline analogs, no single one contained C from CCl4 and only three contained chlorine. Consequently, most adducts would be missed in experiments where formation of reaction products are studied by formation of14C or36Cl labeled adducts (e.g. covalent binding studies used by toxicologists). If similar hydroxyproline analog reaction products were observed during CCl4 intoxication it might be reasonably expected that they interfered with collagen metabolism and participate in cirrhogenic effects of CCl4 on the liver.
    Type of Medium: Electronic Resource
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