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  • 1
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have attempted to optimize conjugation and sporulation in Candida lipolytica, by studying the conditions of culture and growth. Copulation between compatible strains is a rare event, particularly in the case of auxotrophic mutants. However, diploids can be selected for on minimal medium provided parents are suitable auxotrophs. These diploids can multiply vegetatively for many generations. They can also be induced to sporulate at a very high frequency. Free ascospores were isolated by means of paraffin oil and segregations of markers could be studied. At first quite irregular, these segregations improved following a number of brother-sister matings. At the same time, the mean number of spores per ascus as well as spore germinability were considerably increased.
    Type of Medium: Electronic Resource
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