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  • 1
    ISSN: 1440-1681
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: 1. The binding of l,3-[3H]-dipropyl-8-cyclopentylxanthine ([3H]-DPCPX), a specific adenosine Ai receptor antagonist, was examined in rat vas deferens membrane preparations using radioligand binding techniques.2. l,3-[3H]-Dipropyl-8-cyclopentylxanthine bound to these preparations with a KD of 1.07 ± 0.14 nmol/L (n = 6). The density of [3H]-DPCPX binding sites was 133.38 ± 5.57 fmol/mg protein.3. Computer analysis indicated that nucleosides competed for [3H]-DPCPX binding at two distinct sites. The rank order of potency at the higher affinity site corresponded to R-phenyliso-propyladenosine (R-PIA) 〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03051870:CEP492:ges" location="ges.gif"/〉 2-chloroadenosine (2-CI ADO) 〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03051870:CEP492:ges" location="ges.gif"/〉 cyclopentyladenosine (CPA) 〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03051870:CEP492:ges" location="ges.gif"/〉 N-ethylcarboxamidoadenosine (NECA)〉s-phenylisopropyladenosine (s-PIA). Kj values were in the low nmol/L range. The rank order of nucleoside potency at the lower affinity site corresponded to R-PIA 〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03051870:CEP492:ges" location="ges.gif"/〉 CPA 〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03051870:CEP492:ges" location="ges.gif"/〉NECA〉=2-ClADO〉 S-PIA. Ki values were in the low μmol/L range.4. Nucleotides competed for [3H]-DPCPX binding at a single site only. The rank order of potency at this site corresponded to a,β-methylene ATP 〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03051870:CEP492:ges" location="ges.gif"/〉βγ-methylene ATP 〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03051870:CEP492:ges" location="ges.gif"/〉= ATP. Ki values were in the high |xmol/L range. This site seemed to correspond with one of the two binding sites predicted by nucleoside competition binding.5. The ATP-regenerating compound myokinase did not significantly change the competition curve for ATP, indicating that the competition for [3H]-DPCPX binding observed in the presence of ATP was due to an effect of ATP per se and not to an action of a degradation product.6. The results demonstrate that in rat vasa deferentia there exist two distinct binding sites for [3H]-DPCPX. One of these sites binds only nucleosides and may represent an adenosine Ai receptor, as usually defined. The other site binds both nucleosides and nucleotides and may represent an atypical adenosine A1 receptor, an atypical P2 or a P3 purinoceptor.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    ISSN: 1552-6909
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The pace of knowledge development related to genetics continues to progress exponentially. Knowledge gained through the Human Genome Project will have a profound impact on the practice of nursing, and the widespread integration of genetics into nursing education, clinical practice, and research will be essential. Many nurses continue to view genetic services as relegated to a specialist in a tertiary center and not relevant to their practice. This is certainly about to change. This article reviews the major implications of Human Genome Project for changing the landscape of health care and nursing, discusses several specific examples of genetic testing, and describes strategies under way to help prepare nurses to meet these new demands. Understanding new DNA-based genetic-testing technologies will be important for nurses as these tests become more available and nurses assist clients in the management of their genetic health. Nurses must become active participants in embracing the challenges and opportunities related to this genetic revolution, which over the next 5 to 10 years will reach nurses in every specialty and in every practice setting.
    Type of Medium: Electronic Resource
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