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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 21 (1992), S. 83-84 
    ISSN: 1432-0983
    Keywords: Yeast ; Transformation ; Thio compound ; Stationary phase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45°C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5×108 cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 104 transformants/μg plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0983
    Keywords: HBsAg ; PEP4 ; Secretion ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The toxicity of HBsAg in the secretion pathway of pep4 strains can be progressively reduced in modified SD media containing lower concentrations of ammonium sulphate. A procedure, combining a reduction of ammonium sulphate concentration in SD media with the disruption of the PEP4 gene of the host strain, was developed to enrich transformants which are not inhibited by HBsAg expressed in the secretion pathway. Abnormal growth of these non-inhibited transformants is characterized by the enlargement of cell morphology, a transition to pseudohyphal-like growth in nitrogen-starved media, an increase in HBsAg particle production, and the enhancement of growth rate in liquid media. This suggests a new approach to overcoming the toxicity of heterologous protein in the yeast secretion pathway.
    Type of Medium: Electronic Resource
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