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  • 1
    Keywords: Life sciences ; Medical virology ; Animal ecology ; Aquatic biology ; Conservation biology ; Wildlife management ; Environmental Medicine ; Life sciences ; Animal ecology ; Environmental Health ; Fish & Wildlife Biology & Management ; Virology ; Freshwater & Marine Ecology ; Conservation Biology/Ecology ; Springer eBooks
    Description / Table of Contents: Introduction -- Distribution and Phylogeny of Ranaviruses -- Host-pathogen Ecology and Evolution -- Molecular Biology of Ranaviruses -- Immune Evasion and Host Immunity -- Pathology and Diagnostics -- Design and Analysis of Ranavirus Studies -- Global Ranavirus Consortium
    Abstract: The first book of its kind, this work discusses the global extent of ranaviruses, principles of ranavirus ecology and evolution.℗ The research included℗ provides guidance on designing ranavirus surveillance studies to determine risk. Ranaviruses are are double-stranded DNA viruses that cause hemorrhagic disease in amphibians, reptiles, and fish. Ranaviruses have caused mass die-offs of ectothermic vertebrates in wild and captive populations around the globe. Ranaviruses: Lethal Pathogens of Ectothermic Vertebrates serves an urgent need to assemble the contemporary information on ranaviruses, and provide guidance on how to assess℗ this threat in populations
    Pages: X, 246 p. 25 illus., 21 illus. in color. : online resource.
    ISBN: 9783319137551
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  • 2
    ISSN: 1432-1211
    Keywords: Key words Beta-2 microglobulin ; Catfish ; MHC ; Phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1211
    Keywords: Key words Major histocompatibility complex ; Catfish ; Polymorphism ; Expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Four cDNAs encoding the major histocompatibility complex (MHC) class I α chain were isolated from a channel catfish clonal B-cell cDNA library. Sequence analysis suggests these cDNAs represent three different MHC class I loci. All cDNAs encoded conserved residues characteristic of the MHC class I α chain: namely, those involved in peptide binding, salt bridges, disulfide bond formation, and glycosylation. Southern blot analyses of individual outbred and second-generation gynogenetic fish indicated the existence of both polygenic and polymorphic loci. Northern blot studies demonstrated that catfish B, T, and macrophage cell lines transcribed markedly higher levels of class I α and β2-microglobulin (β2m) mRNA than fibroblast cell lines. In addition, immunoprecipitation data showed that a 41 000 M r glycoprotein (presumably class I α) was associated with β2m on the surface of catfish B cells. This latter finding is the first direct evidence for the cell surface association of β2m with the MHC class I α chain on teleost cells and supports the notion that functional MHC class I proteins exist in teleosts.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-0603
    Keywords: B cells ; T cells ; Catfish ; Cell lines ; Leukocytes ; Monocytes/macrophages
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Techniques are described for the generation of channel catfish long term leukocyte cell lines. These techniques include the isolation of peripheral blood leukocytes, purification of B cells by anti-immunoglobulin panning, mitogen stimulation, and in vitro maintenance and cloning of leukocyte cultures. Once stimulated in vitro, channel catfish leukocytes proliferate continuously without the need for exogenous growth factors or feeder cells. The various leukocyte cultures generated are heterogeneous and contain mixtures of monocyte/macrophage-like, T-like, or B cells. Clonal cell lines can be obtained from these mixed cultures by limiting dilution cloning in the presence of conditioned medium. A critical component of the culture medium is the use of channel catfish serum which is required for supporting and maintaining in vitro leukocyte proliferation.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1436-2236
    Keywords: Key words: gynogenetic fish, Ictalurus punctatus, MHC, cytotoxic cells, isogenic lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract: Second-generation gynogenetic channel catfish were characterized by molecular and immunologic assays to determine if they were isogenic at major histocompatibility complex loci. Southern blot analyses, using channel catfish MHC class II B and class I A gene probes, revealed identical banding patterns among second-generation gynogenetic fish. In contrast, banding patterns from outbred fish differed not only from gynogenetic animals, but also among themselves. Nucleotide sequence analysis of the MHC class II β1 domain, which encompasses the peptide binding region, from four randomly selected gynogenetic fish showed a single DNA sequence. In contrast, analysis of the same region from three outbred fish showed sequences that differed not only among themselves, but also from those of gynogenetic animals. In cytotoxic assays, peripheral blood leukocytes from outbred fish lysed both gynogenetic and allogeneic targets, whereas those from gynogenetic fish lysed only allogeneic targets. Taken together, these results suggest that this group of second-generation gynogenetic channel catfish is isogenic at MHC loci and may provide an excellent system with which to study cell-mediated immunity in teleosts.
    Type of Medium: Electronic Resource
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