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  • 1
    Publication Date: 2018-01-11
    Description: Metaproteomics can greatly assist established high-throughput sequencing methodologies to provide systems biological insights into the alterations of microbial protein functionalities correlated with disease-associated dysbiosis of the intestinal microbiota. Here, we utilized the well-characterized murine T cell transfer model of colitis to find specific changes within the intestinal luminal proteome associated with inflammation. MS proteomic analysis of colonic samples permitted the identification of ∼10,000-12,000 unique peptides that corresponded to 5,610 protein clusters identified across three groups, including the colitic Rag1 −/− T cell recipients, isogenic Rag1 −/− controls, and wild-type mice. We demonstrate that the colitic mice exhibited a significant increase in Proteobacteria and Verrucomicrobia and show that such alterations in the microbial communities contributed to the enrichment of specific proteins with transcription and translation gene ontology terms. In combination with 16S sequencing, our metaproteomics-based microbiome studies provide a foundation for assessing alterations in intestinal luminal protein functionalities in a robust and well-characterized mouse model of colitis, and set the stage for future studies to further explore the functional mechanisms of altered protein functionalities associated with dysbiosis and inflammation. This article is protected by copyright. All rights reserved
    Print ISSN: 1615-9853
    Electronic ISSN: 1615-9861
    Topics: Medicine
    Published by Wiley-Blackwell
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  • 2
    Publication Date: 2018-02-24
    Description: DOI: 10.1002/pmic.201700391 In article number 1700391, Moon et al. performed a metaproteomic analysis of colonic samples from a mouse model of distal gut inflammation. The authors employed a T cell transfer model of colitis and directly compared wild-type, immunocompromised isogenic Rag1 −/− and the colitic Rag1 −/− T cell recipient mice to identify alterations in both host and gut microbial proteins associated with intestinal inflammation. Art designer: Judith Umaña Ayala
    Print ISSN: 1615-9853
    Electronic ISSN: 1615-9861
    Topics: Medicine
    Published by Wiley-Blackwell
    Signatur Availability
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