Description / Table of Contents:
PhageFISH for Monitoring Phage Infections at Single Cell Level -- Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria -- Engineering Bacteriophage-Based Biosensors -- Introduction of Phage Genome into Escherichia coli by Electroporation -- Site-Specific Mutagenesis of Bacillus subtilis Phage SPO1 -- Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA -- Isolation of Competitive Phage Display-Modified Bacteriophage T4 with Affinity Chromatography -- Immobilization of Intact Phage and Phage-Derived Proteins for Detection and Biocontrol Purposes -- Peptidoglycan Hydrolytic Activity of Bacteriophage Lytic Proteins in Zymogram Analysis -- Analyzing Phage-Host Protein-Protein Interactions using Strep-tag® II Purifications -- Techniques to Assess Phage/Biofilm Interaction -- Screening for Growth-Inhibitory ORFans in Pseudomonas aeruginosa-Infecting Bacteriophages -- The Use of the greater Wax Moth Larvae (Galleria mellonella) as an Alternative Animal Infection Model for the Analysis of Bacterial Pathogenesis -- Use of a Silkworm Larva Model in Phage Therapy Experiments -- Exploitation of Drosophila Infection models to Evaluate Antibacterial Efficacy of Phages -- Duckweed (Lemna minor) and Alfalfa (Medicago sativa) as Bacterial Infection Model Systems -- Quantitating Phage Efficacy in ready-To-Eat Meats -- Use of a Chicken Embryo Lethality Assay to Assess the Efficacy of Phage Therapy
This book expands on the previous volumes with new chapters focusing on functional characterization of phage and their proteins, and on the development of phage therapy by outlining novel models. The chapters in this book cover molecular topics such as PhageFISH for monitoring phage infections at single cell level; the analysis of phage-host protein-protein interactions using Strep-tag® II purifications; and also application driven chapters including ‘duckweed (Lemna minor) and alfalfa (Medicago sativa) as bacterial infection model systems’. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Innovative and thorough, Bacteriophages: Methods and Protocols, Volume IV is a valuable resource for both established and novice phage scientists
XI, 216 p. 81 illus., 46 illus. in color. : online resource.