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  • 1
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  125. Kongress der Deutschen Gesellschaft für Chirurgie; 20080422-20080425; Berlin; DOC08dgch9789 /20080416/
    Publication Date: 2008-04-14
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 2
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  124. Kongress der Deutschen Gesellschaft für Chirurgie; 20070501-20070504; München; DOC07dgch7227 /20071001/
    Publication Date: 2007-10-02
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 3
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  128. Kongress der Deutschen Gesellschaft für Chirurgie; 20110503-20110506; München; DOC11dgch697 /20110520/
    Publication Date: 2011-05-20
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 4
    Keywords: CANCER ; CELLS ; EXPRESSION ; IN-VITRO ; INVASION ; carcinoma ; CELL ; Germany ; PATHWAY ; PATHWAYS ; VITRO ; GENE ; GENE-EXPRESSION ; GENES ; microarray ; RNA ; SAMPLE ; SAMPLES ; transcription ; COMPONENTS ; TISSUE ; LINES ; TRANSDUCTION ; ACTIVATION ; TISSUES ; BIOLOGY ; CELL-LINES ; fibroblasts ; MOLECULAR-BIOLOGY ; signal transduction ; SIGNAL ; BREAST-CANCER ; PROGRESSION ; immunohistochemistry ; gene expression ; DIFFERENCE ; NUMBER ; METASTASIS ; SIGNAL-TRANSDUCTION ; CELL-LINE ; LINE ; adenocarcinoma ; TARGETS ; MICROARRAY ANALYSIS ; OVEREXPRESSION ; DIFFERENTIAL EXPRESSION ; gene expression profiling ; expression profiling ; microdissection ; pancreatic cancer ; pancreatic carcinoma ; chronic pancreatitis ; WNT ; molecular biology ; molecular ; PANCREATIC-CANCER ; fibroblast ; DUCTAL ADENOCARCINOMA ; PANCREATITIS ; PROTOCOL ; quantitative RT-PCR ; interaction ; analysis ; DIFFERENTIALLY EXPRESSED GENES ; signalling ; WNT pathway ; tumor microenvironment ; ENGLAND ; SET ; MEDICINE ; quantitative ; PROFILE ; pancreatic ductal adenocarcinoma ; response ; interactions ; SFRP1 ; expression profile ; stroma ; in vitro ; ABERRANT METHYLATION ; WNT5a
    Abstract: Pancreatic ductal adenocarcinoma (PDAC) is characterized by an abundant desmoplastic stroma. Interactions between cancer and stromal cells play a critical role in tumour invasion, metastasis and chemoresistance. Therefore, we hypothesized that gene expression profile of the stromal components of pancreatic carcinoma is different from chronic pancreatitis and reflects the interaction with the tumour. We investigated the gene expression of eleven stromal tissues from PDAC, nine from chronic pancreatitis and cell lines of stromal origin using the Affymetrix U133 GeneChip set. The tissue samples were microdissected, the RNA was extracted, amplified and labelled using a repetitive in vitro transcription protocol. Differentially expressed genes were identified and validated using quantitative RT-PCR and immuno-histochemistry. We found 255 genes to be overexpressed and 61 genes to be underexpressed within the stroma of pancreatic carcinoma compared to the stroma of chronic pancreatitis. Analysis of the involved signal transduction pathways revealed a number of genes associated with the Wnt pathway of which the differential expression of SFRP1 and WNT5a was confirmed using immunohistochemistry. Moreover, we could demonstrate that WNT5a expression was induced in fibroblasts during cocultivation with a pancreatic carcinoma cell line. The identified differences in the expression profile of stroma cells derived from tumour compared to cells of inflammatory origin suggest a specific response of the tissue surrounding malignant cells. The overexpression of WNT5a, a gene involved in the non canonical Wnt signalling and chondrocyte development might contribute to the strong desmoplastic reaction seen in pancreatic cancer
    Type of Publication: Journal article published
    PubMed ID: 18298655
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  • 5
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    German Medical Science; Düsseldorf, Köln
    In:  122. Kongress der Deutschen Gesellschaft für Chirurgie; 20050405-20050408; München; DOC05dgch2619 /20050615/
    Publication Date: 2005-06-16
    Keywords: ddc: 610
    Language: German
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  • 6
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  125. Kongress der Deutschen Gesellschaft für Chirurgie; 20080422-20080425; Berlin; DOC08dgch9802 /20080416/
    Publication Date: 2008-04-14
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 7
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  124. Kongress der Deutschen Gesellschaft für Chirurgie; 20070501-20070504; München; DOC07dgch6903 /20071001/
    Publication Date: 2007-10-02
    Keywords: ddc: 610
    Language: German
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 35 (1977), S. 171-177 
    ISSN: 1432-0584
    Keywords: Immunhämolyse ; Penicillinantikörper ; Monozytenfunktion ; Membranrezeptoren ; Immunhemolysis ; Antipenicillin-antibodies ; Function of monocytes ; Membrane-receptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Under certain conditions human monocytes were able to bind and ingest red cell-antibody complexes in vitro. Using penicillincoated red cells and purified monocytes we investigated sera of patients with penicillin allergy. It was shown that sera containing IgG-antibodies against penicillin induced the binding of penicillin-coated red cells to isolated monocytes provided IgG-antibodies of high titer were present. Inhibition and absorption tests demonstrated the specificity of the reaction in terms of IgG-antibodies and the drug. Monocyte binding was also studied in respect to the cross reactivity of penicillin antibodies and cephalosporins. We concluded that antipenicillin-antibodies of the IgG-class were able to induce an immunphagocytosis in vitro, if the drug was present in the test system. The reaction was dependent on the amount of antibodies of the IgG-class.
    Notes: Zusammenfassung Bedingungen zur Bindung von Erythrozyten-Antikörper-Komplexen durch Zellen der Makrophagenreihe wurden an isolierten Monozyten definiert. Unter Verwendung penicillinbeladener Erythrozyten wurden Seren von Patienten mit Penicillinantikörpern in diesem Testsystem untersucht. Patientenseren, die Antikörper gegen dieses Medikament enthielten, lösten eine Bindung penicillinbeladener Erythrozyten an Monozyten aus, wenn es sich um höhertitrige Antikörper der IgG-Klasse handelte. Die Spezifltät der Reaktion wurde durch Hemm- und Absorptionsversuche gesichert. Kreuzreaktionen der Antikörper mit Cephalosporinen erscheinen unter den in-vitro-Bedingungen wahrscheinlich. Es wird geschlossen, daß bei Vorhandensein hochtitriger IgG-Antikörper gegen Penicillin in Gegenwart dieses Medikamentes und normaler Erythrozyten eine immunologisch bedingte Bindung der Erythrozyten an Monozyten in vitro häufig nachweisbar ist, die Abhängigkeit vom Antikörpertiter zeigt.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-0584
    Keywords: Bone marrow ; Immunohistology ; Monoclonal antibodies ; Lymphoproliferative disorders
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cryostat sections of 246 consecutive bone marrow biopsies from 212 patients with lymphoproliferative disease were investigated using a panel of monoclonal antibodies (MOAb's) and an immunoperoxidase technique. Bone marrow involvement was demonstrated by immunohistological examination in 121/160 patients (76%) with non-Hodgkin lymphomas (NHL) and 16/23 patients (70%) with plasma cell malignancies; the definite immunological diagnosis could be performed in 77% and 88%, respectively. Reactivity with the MoAb Ki-67 correlated with clinical parameters: in all cases exhibiting more than 5% positive cells an unfavourable course was seen, independent of the histological subtype. Another MoAb of potential prognostic relevance is KiM4b, which reacts with follicular dendritic cells (FDC). Besides the presence of FDC in germinal center tumors (CB/CC and CC-NHL) we found FDC in a minority of cases with B-CLL (5/44) and IC lymphoma (4/18). In the latter group 3/4 patients showed a favourable clinical course (vs 2/14 without FDC). The MoAb Tü1 could discriminate between the lymphoplasmocytoid (11/12 positive) and the lymphoplasmocytic (0/6 positive) subtype of IC lymphoma and has proven of diagnostic importance. Expression of IL-2 receptors, detected by MoAb anti-Tac (CD25), was demonstrated on leukemic cells from patients with hairy cell leukemia (100%), B-CLL (82%), IC (61%), CC (50%) and CB/CC lymphoma (50%). A considerable number of reactive T-lymphocytes (5%–60% of tissue cells) were identified among the neoplastic B cells with a predominance of CD4+ cells in most cases with NHL, whereas the CD4+/CD8+ ratio was significantly lower in myelomas and non-infiltrated bone marrows. The potential meaning of these findings is discussed. The immunohistological bone marrow analysis represents an important additional method in the diagnostic procedures of lymphoproliferative diseases involving the bone.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-0649
    Keywords: PACS: 42.65.Tg; 03.40.Kf; 42.65.Hw
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. We present a review of our recent theoretical and experimental results on the interaction of incoherent two-dimensional solitary beams in PR SBN crystals. We show that the inherent anisotropy of PR nonlinearity strongly affects the interaction between solitons. Theoretical and experimental results reveal that solitons interacting in a plane perpendicular to the direction of external biasing field always attract, whereas those colliding in a plane of the field exhibit anomalous behaviour. They may experience both attractive and repulsive forces, depending on their mutual separation. We also show that this anisotropy results in the complicated topology of soliton trajectories, featuring periodic collisions, prolonged mutual spiraling and collapse, depending on the initial conditions.
    Type of Medium: Electronic Resource
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