Springer Nature eBook
Description / Table of Contents:
This volume details techniques on the study of Isolation, characterization, and exploration of actinobacteria in industrial, food, agricultural, and environmental microbiology. Chapters cover a wide range of basic and advanced techniques associated with research on isolation, characterization and identification of actinobacteria in soil, sediment, estuarine, water, Saltpan, Mangroves, plants, lichens, sea weeds, sea grass, animals-crab, snail, shrimp. Authoritative and cutting-edge, Methods in Actinobacteriology aims to be a useful practical guide to researches to help further their study in this field.
Type of Medium:
XXV, 747 p. 168 illus., 148 illus. in color.
1st ed. 2022.
Springer Protocols Handbooks,
Isolation of Actinobacteria from Soil and Marine Sediment Samples -- Isolation of Actinobacteria from Water Sources -- Isolation of Actinobacteria from Deep Sea -- Isolation of Actinobacteria from Estuary -- Isolation of Actinobacteria from Sponges -- Isolation of Actinobacteria from Shrimp -- Isolation of Actinobacteria from Coral Reef -- Isolation of Actinobacteria from Earthworm Cast -- Isolation of Actinobacteria from Compost Samples -- Isolation of Gut Actinobacteria from Fecal and Tissue Samples -- Isolation of Gut Actinobacteria from Termites -- Isolation of Gut Actinobacteria from Fishes -- Isolation of Actinobacteria from Mangrove Plants -- Isolation of Actinobacteria from Seaweeds -- Isolation of Actinobacteria from Hills -- Isolation of Endophytic Actinobacteria from Flowers, Fruits, and Seeds of Higher Plants -- Isolation of Actinobacteria from Desert Soils -- Isolation of Epiphytic Actinobacteria from Rhizosphere of Spermatophytes -- Isolation of Epiphytic Actinobacteria from Lichens -- Isolation of Endophytic Actinobacteria from Lichens -- Isolation of Psychrophilic and Psychrotolerant Actinobacteria -- Isolation of Halophilic Actinobacteria from different habitats -- Isolation of Thermophilic Actinobacteria from Different Habitats -- Isolation of Stone Dwelling Actinobacteria -- Cultivation Techniques of Rare Actinobacteria -- Chemo-taxonomical Characterization of Actinobacteria -- Application of Bioinformatic Tools for Phylogenetic Analysis -- Methods for Whole-genome Analysis of Actinobacteria through Bioinformatics Approaches -- Nanopore Based Long Read Sequencing Technology to Obtain Highly Contiguous Whole Genome Sequence of Actinobacterial Genomes like Streptomyces sp. -- Mining genomes of Actinobacteria -- Comparative genomics of Actinobacteria -- Biosynthetic Gene Cluster Analysis in Micromonospora Species Using Antismash: Secondary Metabolite Genome Mining Pipeline -- Biosynthetic Gene Cluster Analysis in Actinobacterial Genera Streptomyces spp. -- PCR based Determination of Secondary Metabolite Genes in Actinobacterial Cultures. Molecular Mass Determination of Bacteriocin by SDS-PAGE analysis -- MALDI-TOF Analysis of Actinobacterial Peptides with Respect to MASCOT Database -- Protocols for Preclinical Evaluation and Molecular Docking of Antimicrobial Compounds from Streptomyces sp -- Drug Likeliness Evaluation, ADME-Toxicity Investigation, Docking modes between the Ligand and the target Enzyme and Active Site Prediction -- Energy-based Pharmacophore Hypothesis Combined with Molecular Simulation Protocol for The Screening Of Bioactive Compounds From the Class of Actinobacteria -- Receptor cavity-based approach combined with Autodock protocol for the screening of antiviral compounds from Streptomyces sp. -- Pharmacophore Based Hypothesis Combined with Molecular Docking Protocol for the Screening Of Anti-Cancer Compounds from Streptomyces sp. -- Methods of Identification and Validation of Drug Target -- Transcriptome profiles of Streptomyces sp. -- Culture of Actinobacteria, Isolation, and Characterization of their Bioactive Compounds -- Miniaturized Production of Bioactive Extracts from Actinobacteria -- Screening, Characterization, and Identification of Antibacterial Compounds From Actinobacteria -- Isolation, Identification, and Screening of Polyene Antifungal Compound Producing Streptomyces sampsonii MDCE7 from agroforestry soil -- Screening of Actinobacterial Cultures for Antimycobacterial Activity using Mycobacterium smegmatis -- Screening of Actinobacterial Extracts/Compounds for Antimycobacterial Activity by Luciferase Reporter Phage (LRP) Assay -- Screening of Actinobacteria for Anti-TB Activity by MicroplateAlamarBlue Assay (MABA) -- Screening of Actinobacteria for anti-TB Activity by Agar Dilution Assay -- Antiplasmodial activity of Halophilic Actinobacteria -- An invitroAntiamoebic Activity of Actinobacteria -- Screening for Antiviral Activity: MTT Assay -- Screening for Anticancer Activity: 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium (MTT) Assay -- Screening for Anticancer Activity: Dual Staining Method -- Screening for Anticancer Activity: Neutral Red Uptake Assay -- Screening for Anticancer Activity: Lactic Acid Dehydrogenase Assay -- Screening for Anticancer Activity: DNA Fragmentation Assay -- Screening for Anticancer Activity: Trypan Blue Exclusion Assay -- In vitro Evaluation of Antimitotic Properties Of Actinobacterial Extracts Using Onion Root Tip Assay -- Screening for Antioxidant Activity: Diphenylpicrylhydrazine (DPPH) Assay -- Screening for Antioxidant Activity: Nitric Oxide Scavenging Assay -- Screening for Antioxidant Activity: Metal Chelating Assay -- Screening for Antioxidant Activity: Total Antioxidant Assay -- Screening for Antioxidant Activity: Hydrogen Peroxide Scavenging Assay -- In vitro Evaluation Of Actinobacterial Extracts For Anti Inflammatory Properties -- Screening and production of anti freeze Proteins from Actinobacteria -- In vitro Evaluation of Actinobacterial Extracts for Immunomodulatory Properties -- Screening of Actinobacteria for Enzyme Inhibitor Activity -- Screening of Actinobacteria for Quorum Sensing Inhibition -- Screening of Actinobacterial Extracts for Anti-biofilm Activity -- Screening of Actinobacteria for Microbial Induced Calcium Precipitation (MICP) -- Immobilization of Actinobacterial Cells – Sodium Alginate and Calcium Chloride Method -- Production of Actinobacteria Amylase by Fermentation in Solid State Using Residues Of Licuri Palm (Syagruscoronata) -- Production Of Lipase By Actinobacteria -- Isolation And Cultivation Of Actinobacteria By Submerged Fermentation For The Production Of Keratinase -- Screening of Cellulase from Actinobacteria -- Screening of Pectinase from Actinobacteria -- Screening of Protease from Actinobacteria -- Screening and analysis of Actinobacterial Bioherbicides For Weed Management -- In vitro Assessment of Actinobacteria for Survivability Under Simulated Gastro-Intestinal Transit -- In vitro Assessment of Actinobacteria for Survivability Under Simulated Gastro-Intestinal Transit -- Screening of Actinobacterial Probiotics by Anti-Pathogenic Activity Test -- Screening and Analysis of Probiotic Actinobacteria in Poultry Farming -- Vitamin B12 Producing Actinobacteria as Probiotics for Poultry Production -- Qualitative and Quantitative Estimation of Phosphate Solubilizing Actinobacteria -- Estimation of Nitrogen Production by Actinobacteria -- Screening of Actinobacteria for Siderophore Production -- Isolation and Screening of Symbiotic Actinobacteria From Root Nodules of Actinorhizal plant Casuarina sp -- Biocontrol Activity of Actinobacteria Against Plant Pathogens -- An In vitro nematicidalactivity ofactinobacteria- Juvenile mortality and Egg hatching inhibition -- Estimation of Auxin Production by Actinobacteria -- Screening of Actinobacteria for Mosquitocidal Activity -- Extraction, Characterization, and Identification of Odorous Metabolites from Streptomyces -- Screening of Actinobacteria for Biosurfactant Production -- Optimization and Characterization of Biosurfactant from Streptomyces -- Production of Vitamin B12from Streptomyces species -- Screening of Microbes for the Production of Pigment (Melanin) -- Exploration and Characterization ofMelanin Pigment Produced by Actinomycetes -- Isolation and Production of Prodigiosin Pigments from Streptomyces spp. -- Aerobic and Anaerobic Decolourization of Textile Dyes Using Actinobacteria -- Bioleaching of heavy metals from e-waste using actinobacteria -- Biosynthesis and Characterization of Silver Nanoparticles From Actinobacteria -- Biosynthesis and Characterization of Gold Nanoparticles from Actinobacteria -- Antimicrobial Activity of Extracellular Green-Synthesized Nanoparticles By Actinobacteria -- Antibiofilm Activity of Extracellular Green-Synthesized Nanoparticles By Actinobacteria -- Cytotoxic Activity of Extracellular Green Synthesized Nanoparticles by Actinobacteria -- Sp