Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    facet.materialart.
    facet.materialart.
    German Medical Science GMS Publishing House; Düsseldorf
    In:  Deutscher Kongress für Orthopädie und Unfallchirurgie; 71. Jahrestagung der Deutschen Gesellschaft für Unfallchirurgie, 93. Tagung der Deutschen Gesellschaft für Orthopädie und Orthopädische Chirurgie, 48. Tagung des Berufsverbandes der Fachärzte für Orthopädie; 20071024-20071027; Berlin; DOCW51-643 /20071009/
    Publication Date: 2007-10-10
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    facet.materialart.
    facet.materialart.
    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Zeitschrift zur Förderung der Qualitätssicherung in medizinischen Laboratorien; VOL: 7; DOC01 /20160324/
    Publication Date: 2016-03-25
    Description: Patient near diagnostics ensure the monitoring of the individual hemostatic capacity of surgery and intensive care patients. Therefor thrombelastometry provideds a series of standard measurements. The challenge for such a bedside diagnostic method is that it can be performed by "laboratory-unexpierenced" staff, and special attention has to be paid to result validation. External quality controls with External Quality Assessment Schemes (EQAS) provide an appropriate instrument, which was established for the thrombelastometry. The increasing number of participants and good pass rates show the relevance of this topic. Here we present a summary of the results and experiences of four consecutive EQAS. With average inter laboratory coefficients of variation (CVs) of 〈20% the results are within the range of typical physiological plasma clotting methods.
    Description: Patientennahe Diagnostik sichert die Überwachung der individuellen Hämostasekapazität von Patienten der Operativen und der Intensivmedizin. Die Thrombelastometrie bildet hierfür ein standardisiertes Spektrum von Messgrößen an. Die Herausforderung dieser schnellen diagnostischen Methode, die von "laborunerfahrenem" Personal durchgeführt werden kann, bedeutet andererseits, dass ein besonderes Augenmerk auf valide Ergebnisse gelegt werden muss. Die externe Qualitätssicherung über Ringversuche stellt dafür ein probates Mittel dar, die für die Thrombelastometrie etabliert wurde. Die steigenden Teilnehmerzahlen und guten Bestehensquoten zeigen die hohe Relevanz dieser Thematik. Wir präsentieren hier eine Zusammenfassung der Ergebnisse und Erfahrungen aus vier konsekutiven Ringversuchen. Mit mittleren Interlabor-Variationskoeffizienten (VKs) von 〈20% bewegen sich die Ergebnisse im Bereich der gerinnungsphysiologischen Plasmamethoden.
    Keywords: EQAS ; ROTEM ; external quality control ; InTEM ; ExTEM ; Ringversuche ; ROTEM ; externe Qualitätskontrolle ; InTEM ; ExTEM ; ddc: 610
    Language: German
    Type: article
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    facet.materialart.
    facet.materialart.
    German Medical Science GMS Publishing House; Düsseldorf
    In:  21. Jahrestagung der Deutschen Gesellschaft für Pädiatrische Infektiologie (DGPI); 20130425-20130427; Würzburg; DOC13dgpi32 /20130328/
    Publication Date: 2013-03-29
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    Keywords: EXPRESSION ; GENE ; ACQUISITION ; MUTATION ; CHROMOSOMAL LOCALIZATION ; ALOX12B ; GENOMIC STRUCTURE ; RECESSIVE CONGENITAL ICHTHYOSIS ; ADIPOCYTE DIFFERENTIATION ; 12R-LIPOXYGENASE
    Abstract: Loss-of-function mutations in the lipoxygenase (LOX) genes ALOX12B and ALOXE3 are the second most common cause of autosomal recessive congenital ichthyosis. The encoded proteins, 12R-LOX and epidermal LOX-3 (eLOX-3), act in sequence to convert fatty acid substrates via R-hydroperoxides to specific epoxyalcohol derivatives and have been proposed to operate in the same metabolic pathway during epidermal barrier formation. Here, we show that eLOX-3 deficiency in mice results in early postnatal death, associated with similar but somewhat less severe barrier defects and morphological changes than reported earlier for the 12R-LOX-knockout mice. Skin lipid analysis demonstrated that the severity of barrier failure is related to the loss of covalently bound ceramides in both 12R-LOX- and eLOX-3-null mice, confirming a proposed functional linkage of the LOX pathway to ceramide processing and formation of the corneocyte lipid envelope. Furthermore, analysis of free oxygenated fatty acid metabolites revealed strongly reduced levels of hepoxilin metabolites in eLOX-3-deficient epidermis, indicating an additional function of eLOX-3 in mammalian skin as a hepoxilin synthase linked to the 12S-LOX pathway.
    Type of Publication: Journal article published
    PubMed ID: 22832496
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    Keywords: CELLS ; GENES ; DIFFERENTIATION ; MICE ; PPAR-GAMMA ; placenta ; TROPHOBLAST ; MASH2
    Abstract: Metabolites of the epidermal lipoxygenase-3 (eLOX-3) are involved in various metabolic pathways. Most unexpectedly, intra-amniotic delivery of eLOX-3 to mice at gestational day 14.5, both via an adenoviral vector and as recombinant protein, resulted in fetal growth restriction and intrauterine death. Periodic acid-Schiff staining and RT-PCR analysis of placentae from fetuses exposed to eLOX-3 indicated a lack of glycogen trophoblasts in the junctional zone. Placenta-specific gene expression was altered. Thus, the observed prenatal toxicity of eLOX-3 could be due to a strong effect on placental development.
    Type of Publication: Journal article published
    PubMed ID: 25043671
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    Keywords: SYSTEM ; TOOL ; validation ; INDEX ; score ; CONSENSUS ; DERMATITIS ; AREA ; ATOPIC ECZEMA SEVERITY ; OUTCOME MEASURES
    Abstract: Background Autosomal recessive congenital ichthyoses (ARCIs) are keratinization disorders caused by impaired skin barrier function. Mutations in the genes encoding the lipoxygenases 12R-LOX and eLOX-3 are the second most common cause of ARCIs. In recent years, human skin equivalents recapitulating the ARCI phenotype have been established. Objectives To develop a murine organotypic tissue culture model for ARCI. Methods Epidermal keratinocytes were isolated from newborn 12R-LOX-deficient mice and cocultivated with mouse dermal fibroblasts embedded in a scaffold of native collagen type I. Results With this experimental set-up the keratinocytes formed a well-organized multilayered stratified epithelium resembling skin architecture in vivo. All epidermal layers were present and the keratinocytes within showed the characteristic morphological features. Markers for differentiation and maturation indicated regular epidermal morphogenesis. The major components of epidermal structures were expressed, and were obviously processed and assembled properly. In contrast to their wild-type counterparts, 12R-LOX-deficient skin equivalents showed abnormal vesicular structures in the upper epidermal layers correlating with altered lipid composition and increased transepidermal water loss, comparable with 12R-LOX-deficient mice. Conclusions The mouse skin equivalents faithfully recapitulate the 12R-LOX-deficient phenotype observed in vivo, classifying them as appropriate in vitro models to study molecular mechanisms involved in the development of ARCI and to evaluate novel therapeutic agents. In contrast to existing human three-dimensional skin models, the generation of these murine models is not constrained by a limited supply of material and does not depend on in vitro expansion and/or genetic manipulations that could result in inadvertent genotypic and phenotypic alterations.
    Type of Publication: Journal article published
    PubMed ID: 25078898
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    facet.materialart.
    facet.materialart.
    German Medical Science GMS Publishing House; Düsseldorf
    In:  VI. International Symposium on AMD - Age-Related Macular Degeneration - Emerging Concepts - Exploring known and Identifying new Pathways; 20150911-20150912; Baden-Baden; DOC15amd19 /20151001/
    Publication Date: 2015-10-02
    Keywords: ddc: 610
    Language: English
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
  • 9
    ISSN: 1432-1106
    Keywords: Pulvinar ; Area 17 ; Prestriate cortex ; Thalamo-cortical organization ; Callithrix jacchus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In eleven hemispheres of nine marmoset monkeys (Callithrix jacchus), we have investigated the thalamo-cortical organization of the projections from the pulvinar to the striate and prestriate cortex. In each experiment, single or multiple injections of various retrograde fluorescent tracers were injected into adjacent regions or areas. In two experiments, horseradish peroxidase (HRP) was injected into the lateral geniculate nucleus (LGN) and the lateral pulvinar, respectively. The results show that the thalamo-cortical projection from LGN to striate cortex and from pulvinar to the prestriate cortex are similarly organized, but the geniculo-striate projection is more precise than the pulvinar-prestriate projection. The pulvinar-prestriate projection is topographically organized and preserves topological neighbourhood relations. Projection zones to the various visual areas are concentrically wrapped around each other. The projection zone to area 18 constitutes a central core region. It begins ventro-laterally in PuL where the pulvinar is in contact with the LGN. This contact zone we called the hilus region of the pulvinar. The area 18-projection zone stretches as a central cone into the posterior pulvinar through PuL and into PuM. It is surrounded by the projection zone to the posterior belt of area 19 and this in turn is surrounded by the projection zone to the anterior belt of area 19. The projection zones to area 19 are then surrounded medially and dorsally by zones projectiong to the temporal and parietal association cortex, respectively. The projection zone to area MT is located medio-ventrally in the posterior pulvinar (PuIP and surrounding nuclei) and coincides with a densely myelinated region. Area 17 also receives input from the pulvinar but probably predominantly in the region of the central visual field. The pulvinar zone projecting to area 17 is located ventrolaterally from the central core region projecting to area 18 and is contiguous laterally with the LGN. If the positions of the vertical and the horizontal meridian in the pulvinar correspond to those in the respective cortical projection zones, a second order visual field representation such as found in area 18, with the horizontal meridian split at an excentricity of about 7–10°, can also be recognized in the pulvinar.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    ISSN: 1432-0983
    Keywords: Aspergillus niger ; Transformation ; Integration ; pyrA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A homologous transformation system for the filamentous fungus Aspergillus niger has been developed, based on the orotidine-5′-phosphate-decarboxylase gene. A. niger Pyr− mutants have been selected from 5-fluoroorotic acid resistant mutants. These mutants were found to comprise two complementation groups, pyrA and pyrB. The A. niger OMP-decarboxylase gene was isolated from a gene library by heterologous hybridization with the Neurospora crassa pyr4 gene. The cloned gene is capable to transform A. nidulans pyrG mutants at high frequencies. Transformation of A. niger pyrA mutants occurs with moderate frequencies (about 50 transformants/μg DNA) whereas the pyrB mutants cannot be complemented with the cloned OMP-decarboxylase gene. Analysis of the DNA of the A. niger PyrA+ transformants showed that transformation resulted in integration of the vector DNA into the genome by homologous recombination. Both gene replacements and integration of one or more copies of the complete vector have been observed.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...