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  • 1
    Abstract: The molecular biology section of the Hereditary Non-Polyposis Colorectal Cancer study group-Germany, instituted a multicenter study to test the reliability and quality of microsatellite instability (MSI) analysis. Eight laboratories compared MSI analyses performed on 10 matched pairs of normal and tumor DNA from patients with colorectal carcinomas. A variety of techniques were applied to the detection of microsatellite changes: (a) silver and ethidium bromide staining of polyacrylamide gels; (b) radioactive labeling; and (c) automated fluorescence detection. The identification of highly unstable tumors and tumors without MSI was achieved in high concordance. However, the interpretation of the band patterns resulted in divergent classifications at several microsatellite marker loci for a large fraction of this tumor/normal panel. The data on more than 30 primers per case suggest that the enlargement of the microsatellite panel to more than 10 loci does not influence the results. In this study, cases with MSI in less than 10% of loci were classified as microsatellite stable, whereas MSI was diagnosed in cases with more than 40% of all markers unstable. We propose that a panel of five microsatellite loci consisting of repeats with different lengths should be analyzed in an initial analysis. When less than two marker loci display shifts in the microsatellite bands from tumor DNA, the panel should be enlarged to include an additional set of five marker loci. The number of marker loci analyzed as well as the number of unstable marker loci found should always be identified. These criteria should result in reports of MSI that are more comparable between studies.
    Type of Publication: Journal article published
    PubMed ID: 9354434
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  • 2
    ISSN: 1433-0415
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Derzeit sterben jährlich etwa 25000 Männer und 24000 Frauen in der Bundesrepublik Deutschland an Krebs. Die Wahrscheinlichkeit an Krebs zu erkranken steigt mit dem Alter ex- ponentiell an (Abb. 1). Bis zum 65sten Lebensjahr sind erst knapp die Hälfte aller Krebstodesfälle aufgetreten. Bei gleichbleibender Krebsinzidenz in den 80er Jahren und aufgrund der Veränderung in der Altersstruktur der Bevölkerung behandeln wir heutzutage immer häufiger die älteren, über 70jährigen Patienten wegen Krebserkrankungen. [36]. Deshalb spielt das höhere Lebensalter krebskranker Patienten auch in der chirurgischen Therapie eine wesentliche Rolle. Dabei sollten objek-tivierbare Kriterien und nicht die subjektiven Eindrücke des Arztes die Therapieplanung bestimmen [35]. Annähernd ein Viertel der 1994 bis 1996 von uns behandelten Patienten waren älter als 70 Jahre.
    Type of Medium: Electronic Resource
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