Analytical Chemistry and Spectroscopy
Wiley InterScience Backfile Collection 1832-2000
Chemistry and Pharmacology
Two methods are examined which allow a NOESY experiment to be performed involving only a specific site of the molecule labelled by a heteronucleus. They involve the merging of a classical phase-sensitive NOESY experiment with either an INEPT sequence, which transfers 1H magnetization to a coupled heteronucles, or a multiple quantum filtering sequence which allows the selection of only the 1H resonances which are coupled to the heteronucles. The two methods are first compared on two small molecules, non-labelled alanine and [U-13C]alanine, particularly with respect to the signal-to-noise ratio. An example of the application of these methods on a 25000 molecular weight protein system (elastase-peptide complex) is also given. The limitations of the methods, in particular in terms of T2, are discussed.
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