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  • 1
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    Stuttgart : Wissenschaftliche Verlagsgesellschaft
    Call number: QH308:13(8)
    Pages: x, 655 p. : ill.
    Edition: 8., völlig neu bearb. u. erw. Aufl.
    ISBN: 9783804732612
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  • 2
    Call number: 05-BCH-60:42/1
    Pages: 93 p. : ill.
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  • 3
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    Stuttgart : Wissenschaftliche Verlagsgesellschaft
    Call number: QH442:203(3)
    Keywords: Genetic Techniques ; Biotechnology
    Pages: xviii, 725 p. : ill.
    Edition: 3. völlig neu bearb. Aufl.
    ISBN: 9783804737082
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  • 4
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    Weinheim : Wiley-VCH
    Call number: 05-BCH-60:42/2
    Pages: p. 97-177 : ill.
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  • 5
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    Stuttgart : Wiss. Verl.-Ges.
    Call number: QR181:94(2)
    Keywords: Immunology
    Edition: 2., völlig neu bearb. u.erw. Aufl.
    ISBN: 9783804728424
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  • 6
    ISSN: 1432-0983
    Keywords: Dictyostelium discoideum ; RNA polymerase III ; Transcription factors ; tRNA genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary RNA Polymerase III transcription factors from the cellular slime mold Dictyostelium discoideum were characterized, based on their stable binding to isolated tRNA genes. Different protein complexes are sequestered on DNA fragments containing tRNA genes depending on the conditions by which the nuclei were extracted. Binding specificity was determined through competition assays using competitor tRNA genes from the same gene family, from different gene families and from truncated tRNA genes. The complex with the highest multiformity of interdependent proteins is able to assemble with low affinity on a B-block-free tDNA template, whereas most lower molecular weight complexes require the presence of an intact B-block promoter element in order to assemble.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary In order to test the biotechnological potential of the cellular slime mould Dictyostelium discoideum the cDNA coding for human antithrombin III was expressed in this microorganism. The 1392-bp antithrombin III cDNA was fused to the N-terminal coding part of the D. discoideum actin 6 gene. In constructs carrying this artificial N-terminal coding region only low amounts of antithrombin III were detected. However, constructs from which all actin coding nucleotides were removed produced significant amounts of antithrombin III, most of which was secreted into the culture broth. Stationary cultures (1.5 × 107 cells/ml) of certain stable transformants accumulated up to 1.0 μg antithrombin III/ml culture medium within 24 h. The recombinant protein has a slightly smaller molecular weight in sodium dodecyl sulphate-polyacrylamide gels than authentic plasma antithrombin III and it is glycosylated, as determined by concanavalin A labelling.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 0192-253X
    Keywords: Transformation ; Dictyostelium discoideum ; UMP-synthase/ura-complementation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Whereas transformation ofDictyostelium discoideum now can be done routinely and reliably, there is increasing demand for a system that allows selection of cells that have gone through repeated transformation cycles. Such a system is presented here. Selection is based on resistance to 5-fluoro-orotic-acid (5-FOA). D. discoi-deum is highly sensitive to this drug, and cells can survive only in the presence of 5-FOA if they carry a defective UMP-synthase gene. After transformation with a plasmid carrying a cloned version of the UMP-synthase gene, 5-FOA resistant cells are obtained at high frequency. Because 5-FOA-resistant cells depend on exogenously added uracil, these cells can be taken through a second round of transformation. A plasmid-borne UMP-synthase gene renders 5-FOA-resistant cells phenotypically ura +. Finally, cells can be further transformed using the standard G418 selection system.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 11 (1990), S. 410-417 
    ISSN: 0192-253X
    Keywords: Suppressor †RNA genes ; yeast ; lacZ expression in D. discoideum ; †RNATrp genes ; †RNAGlu genes ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We describe the generation of Dictyostelium discoideum cell lines that carry different suppressor †RNA genes. These genes were constructed by primer-directed mutagenesis changing a †RNATrp(CCA) gene from D. discoideum to a †RNATrp(amber) gene and changing a †RNAGlu(UUC) gene from D. discoideum to a †RNAGlu(ochre) as well as a †RNAGlu(amber) gene. These genes were stably integrated into the D. discoideum genome together with a reporter gene. An actin 6::lacZ gene fusion carrying corresponding translational stop signals served as a reported. Active β-galactosidase is expressed only in D. discoideum strains that contain, in addition to the reporter, a functional suppressor †RNA. Both amber suppressors are active in D. discoideum without interfering significantly with cell growth and development. We failed, however, to establish cell lines containing a functional †RNAGlu(ochre) suppressor. This may be due to the fact that nearly every message from D. discoideum known so far terminates with UAA. Therefore a †RNA capable of reading this termination codon may not be compatible with cell growth.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1617-4623
    Keywords: Dictyostelium discoideum ; Restriction fragment length polymorphism ; tRNA genes ; Chromosomal organization ; Milti-copy genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Different wild-type isolates of Dictyostelium discoideum exhibit extensive polymorphism in the length of restriction fragments carrying tRNA genes. These size differences were used to study the organisation of two tRNA gene families which encode a tRNAVal(GUU) and a tRNAVal(GUA) gene. The method used involved a combination of classitics. The tRNA genes were mapped to specific linkage groups (chromosomes) by correlating the presence of polymorphic DNA bands that hybridized with the tRNA gene probes with the presence of genetic markers for those linkage groups. These analyses established that both of the tRNA gene families are dispersed among sites on several of the chromosomes. Information of nine tRNAVal(GUU) genes from the wild-type isolate NC4 was obtained: three map to linkage group I (C, E, F,), two map to linkage group II (D, I), one maps to linkage group IV (G), one, which corresponds to the cloned gene, maps to either linkage group III or VI (B), and two map to one of linkage groups III, VI or VIII (A, H). Six tRNAVal(GUA) genes from the NC4 isolate were mapped; one to linkage group I (D), two to linkage group III, VI or VII (B, C) and three to linkage group VII or III (A, E, F).
    Type of Medium: Electronic Resource
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