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  • 1
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Repetitive DNA sequences in and around the rat prolactin gene were identified and mapped by hybridizing labeled total rat DNA to cloned prolactin DNA and by hybridizing labeled prolactin DNA fragments to total restricted rat DNA. Repeated sequences were found 12-8 and 2.5-0.5 kb upstream of the first exon, within the fourth intron and throughout almost the entire 15.5 kb of cloned DNA downstream from the last exon. By cross-hybridization of the major repeated sequences, it was found that these repeats could be grouped into three families, one of which is novel. Human alu and mouse alu-like probes did not hybridize to the rat prolactin DNA. Several of the repeats from the rat prolactin gene hybridized to the intron and downstream flanking regions of the rat growth hormone gene.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0730-2312
    Keywords: gene regulation ; 2,3,7,8-tetrachlorodibenzo-p-dioxin ; cytochrome P1-450 ; receptor ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) dioxin, produces a diverse set of biological responses which, in some cases, reflects the altered expression of specific genes. An intracellular receptor protein binds TCDD saturably and with high affinity and mediates several of TCDD's biological effects. In mouse hepatoma cells, TCDD induces aryl hydrocarbon hydroxylase activity by activating the transcription of the cytochrome P1-450 gene. Studies of receptor-defective variant cells indicate that the activation of cytochrome P1-450 gene transcription requires functional TCDD receptors. Analysis of the DNA that flanks the 5′-end of the mouse cytochrome P1-450 gene reveals at least three control regions: a promoter, an inhibitory element, and a dioxin-responsive element (DRE). Therefore, expression of the cytochrome P1-450 gene represents a balance between negative and positive control. The DRE contains two discrete, non-overlapping DNA domains that respond to TCDD. Each TCDD-responsive domain acts independently of the other, each requires TCDD receptors for function, and each has the properties of a transcriptional enhancer. For example, the function of the DREs is relatively independent of both their location and their orientation with respect to the promoter. Together, the DREs and the TCDD-receptor complex constitute a dioxin-responsive enhancer system. Exposure of cells to TCDD results in the protection of a specific DNA domain from exonuclease digestion. This protection requires TCDD receptors. The protected domain maps to a DRE. This observation implies that the TCDD-receptor complex interacts with the DRE to activate the transcription of the cytochrome P1-450 gene.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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