Blackwell Publishing Journal Backfiles 1879-2005
By using PCR, we have found previously that differences in T cell receptor alpha/beta chain variable region (TCR α/β) gene expression in lymph nodes (LN) existed between susceptible and resistant mice following anterior chamber (AC) inoculation with herpes simplex virus (HSV). We now report and compare the immunoglobulin gene variable region (Ig VH) and the TCR Vβ mRNA expression in normal congenic mice (BALB/c and C. B-17) and also in B cell modulated C. B-17 mice (B-). RNA prepared from spleen and LN of these mice before and after HSV-AC inoculation was analysed by PCR using oligoprimers specific for 11 VH gene families and 19 Vβ gene families. Densitometry analysis revealed that VH family mRNA expression levels in spleen and lymph nodes did not correlate with HSV susceptibility or resistance patterns in B-, BALB/c and C. B-17 mice. Analysis of TCR Vβ chain genes showed that spleen of resistant C. B-17 and B- Ab transferred mice showed a preference for Vβl 1 gene expression not seen in susceptible mice. In contrast, LN of BALB/c and B-, both susceptible mice, made TCR Vβ transcripts that were indistinguishable from those generated by resistant C. B-17 mice, following HSV-AC inoculation. Finally, TCR Vβ gene family repertoire appears to be severely diminished in uninfected B- mice (50% in LN and 45% in spleen) by anti-μ treatment.
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