Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    facet.materialart.
    facet.materialart.
    German Medical Science GMS Publishing House; Düsseldorf
    In:  57th Annual Meeting of the German Society for Neuropathology and Neuroanatomy (DGNN); 20120912-20120915; Erlangen; DOC12dgnnPP7.3 /20120911/
    Publication Date: 2012-09-12
    Keywords: ddc: 610
    Language: English
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    facet.materialart.
    facet.materialart.
    German Medical Science GMS Publishing House; Düsseldorf
    In:  Mainz//2011; 56. Jahrestagung der Deutschen Gesellschaft für Medizinische Informatik, Biometrie und Epidemiologie (gmds), 6. Jahrestagung der Deutschen Gesellschaft für Epidemiologie (DGEpi); 20110926-20110929; Mainz; DOC11gmds124 /20110920/
    Publication Date: 2011-09-20
    Keywords: Hysterektomie ; MARZY-Studie ; Überlebenszeitanalyse ; ddc: 610
    Language: German
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
  • 4
    Keywords: RECEPTOR ; ANGIOGENESIS ; APOPTOSIS ; CELLS ; ENDOTHELIAL-CELLS ; EXPRESSION ; GROWTH ; GROWTH-FACTOR ; IN-VITRO ; proliferation ; tumor ; CELL-PROLIFERATION ; FACTOR RECEPTOR ; Germany ; IN-VIVO ; INHIBITION ; MODEL ; PERFUSION ; VITRO ; imaging ; HEPATOCELLULAR-CARCINOMA ; GENE ; GENES ; METABOLISM ; TUMORS ; LINES ; TRANSDUCTION ; gene transfer ; GENE-TRANSFER ; DNA ; INDUCTION ; CELL-LINES ; signal transduction ; immunohistochemistry ; MALIGNANCIES ; ASSAY ; DESIGN ; VECTOR ; NUMBER ; STRESS ; SIGNAL-TRANSDUCTION ; LINE ; positron emission tomography ; POSITRON-EMISSION-TOMOGRAPHY ; tomography ; OXIDATIVE STRESS ; cell lines ; MALIGNANCY ; OXIDATIVE-STRESS ; TUMOR-GROWTH ; monitoring ; endothelial cells ; cell proliferation ; MIGRATION INHIBITORY FACTOR ; CHIP ; computer-assisted ; functional imaging ; ANGIOGENESIS IN-VIVO ; GLIOBLASTOMA GROWTH ; IRRADIATED SKIN ; SOLUBLE FORM ; SYMPORTER GENE
    Abstract: Purpose: Inhibition of tumor angiogenesis is emerging as a promising target in the treatment of malignancies. Therefore, monitoring of antiangiogenic approaches with functional imaging and histomorphometrical analyses are desirable to evaluate the biological effects caused by this treatment modality. Experimental Design: Using a bicistronic retroviral vector for transfer of the soluble receptor for the vascular endothelial growth factor (sFLT) hepatoma (MH3924A) cell lines with sFLT expression were generated. In human umbilical vein endothelial cells cultured with conditioned medium of sFLT-expressing hepatoma cells, the inhibitory action of secreted sFLT was determined using a Coulter counter and a thymidine incorporation assay. Furthermore, in vivo experiments were done to measure the effects on tumor growth and perfusion. Finally, the tumors were examined by immunohistochemistry (including computer-assisted morphometry) and DNA chip analysis. Results: Stable sFLT-expressing hepatoma cells inhibited endothelial cell proliferation in vitro. In vivo, growth and perfusion, as measured by (H2O)-O-15 positron emission tomography, were reduced in genetically modified tumors. However, the immunohistochemically quantified microvascularization and macrovascularization, as indicated by CD31- and alpha-actin-positive area, revealed no significant changes, whereas the number of apoptotic cells was increased in sFLT-expressing tumors, although not significantly. DNA chip analysis of tumors with gene transfer showed an increase of genes related to apoptosis, signal transduction, and oxidative stress. Conclusion: Our results suggest that sFLT expression inhibits tumor growth and perfusion and enhances expression of apoptosis-related genes in this model. Enhanced expression of genes for signal transduction, stress, and metabolism indicates tumor defense reactions
    Type of Publication: Journal article published
    PubMed ID: 15788658
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    facet.materialart.
    facet.materialart.
    German Medical Science GMS Publishing House; Düsseldorf
    In:  24. Jahrestagung der Deutschen Gesellschaft für Pädiatrische Infektiologie (DGPI); 20160428-20160430; Frankfurt am Main; DOC16dgpi10 /20160428/
    Publication Date: 2016-04-29
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    Keywords: ANGIOGENESIS ; APOPTOSIS ; CELLS ; ENDOTHELIAL-CELLS ; EXPRESSION ; GROWTH ; IN-VITRO ; INHIBITOR ; proliferation ; tumor ; ANGIOSTATIN ; BLOOD ; CELL ; CELL-PROLIFERATION ; CLINICAL-TRIAL ; Germany ; human ; IN-VIVO ; INHIBITION ; MICROVESSEL DENSITY ; PERFUSION ; VITRO ; VIVO ; DENSITY ; imaging ; INFORMATION ; QUANTIFICATION ; SUPPORT ; VOLUME ; GENE ; GENES ; METABOLISM ; cell line ; TUMORS ; gene therapy ; LINES ; NUCLEAR-MEDICINE ; TRANSDUCTION ; MECHANISM ; BLOOD-FLOW ; mechanisms ; SUFFICIENT ; CELL-LINES ; signal transduction ; treatment ; SIGNAL ; ACID ; TARGET ; MALIGNANCIES ; STRESS ; SIGNAL-TRANSDUCTION ; CELL-LINE ; LINE ; HEPATOMA ; POSITRON-EMISSION-TOMOGRAPHY ; PET ; OVEREXPRESSION ; cell lines ; nuclear medicine ; TUMOR ANGIOGENESIS ; VASCULARIZATION ; INHIBITORS ; radiology ; MALIGNANCY ; TUMOR-GROWTH ; LEADS ; endothelial cell ; endothelial cells ; ARRAY ; cell proliferation ; TUMOR PERFUSION ; methods ; NUCLEAR ; USA ; EVALUATE ; in vivo ; ENDOTHELIAL-CELL ; blood supply ; MULTIPLE GENES ; blood volume ; coculture ; gene array ; LEWIS LUNG-CARCINOMA ; MEDICINE
    Abstract: Growth of malignant tumors is dependent on sufficient blood supply. Thus, inhibition of tumor angiogenesis is emerging as a promising target in the treatment of malignancies. Human angiostatin (hANG) is one of the most potent inhibitors of endothelial cell proliferation, angiogenesis, and tumor growth in vivo. However, its mechanisms operating in vivo are not well understood. Methods: To obtain more information about functional changes in the angiogenic process, we established Morris hepatoma (MH3924A) cell lines expressing hANG (hANG-MH3924A). The effects of hANG expression on proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs) were measured in coculture experiments in vitro. To evaluate changes in tumor perfusion and blood volume, (H2O)-O-15 and Ga-68-DOTAalbumin (DOTA is 1,4,7,10-tetraazacyclododecane-N,N',N '',N'''tetraacetic acid) were used for PET studies in vivo. Additionally, immunohistologic quantification of vascularization, apoptosis, and proliferation as well as gene array analyses were performed. Results: Our in vitro experiments demonstrate reduced proliferation and increased apoptosis in HUVECs when being co-cultured with hANG-MH3924A. In support, tumor growth of hANG-MH3924A is diminished by 95% in vivo. However, tumor perfusion and blood volume are increased in hANG-MH3924A corresponding to an increased microvessel density. Furthermore, hANG-transfected tumors show changes in expression of genes related to apoptosis, stress, signal transduction, and metabolism. Conclusion: hANG expression leads to inhibition of tumor growth, increased apoptosis, and changes in the expression of multiple genes involved in stress reactions, signal transcluction, and apoptosis, which indicates a multifactorial reaction of tumors. An enhanced microvessel density is seen as part of these reactions and is associated with increased perfusion as measured by PET
    Type of Publication: Journal article published
    PubMed ID: 16513625
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  A novel monoclonal antibody (mAb), 8D3 (IgG2a), that specifically recognizes the murine transferrin receptor (TfR) was produced by immunizing a Lewis rat with a polyoma middle T oncogene-transformed endothelioma cell line. The 8D3 mAb was obtained by immunohistochemical screening for exclusive staining of vessels forming a blood–brain barrier (BBB), but not of other vessels. The anti-TfR mAb 8D3 recognizes the TfR also in FACS analysis and in western blots and should prove to be useful for affinity purification of the TfR. Whereas 8D3 brightly stains BBB-forming vessels in the central nervous system of mice, it does not stain the fenestrated capillaries within the choroid plexus and the circumventricular organs. In testis, where the blood–tissue barrier is located at the level of the Sertoli cells, the 8D3 mAb specifically stains Sertoli cells but not endothelial cells. Finally, in vitro, 8D3 does not interfere with iron uptake of lymphocytes as it does not influence their proliferation. Taken together, 8D3 represents a versatile new tool to study the tissue distribution of the murine TfR and TfR-mediated transcytosis across tissue barriers in the mouse.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Psychosomatic Research 28 (1984), S. 221-229 
    ISSN: 0022-3999
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine , Psychology
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 9
    ISSN: 0340-1855
    Keywords: Schlüsselwörter Adhäsionsmolekül – T-Zelle – Multiple-Skerlose – Experimentelle autoimmune Enzephalomyelitis (EAE) – Blut-Hirn-Schranke ; Key words Cell adhesion molecule – T cell – multiple sclerosis – experimental autoimmune encephalomyelitis (EAE) – blood brain barrier
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Until recently, the central nervous system (CNS) has been considered to be an immunologically privileged site, where the highly specialized endothelial blood-brain barrier (BBB) allows no entry of circulating lymphocytes. However, during inflammation in the CNS, mononuclear cells readily gain access to the CNS parenchyme. The molecular mechanisms involved in lymphocyte recruitment across the BBB have been mostly studied in experimental autoimmune encephalomyelitis (EAE), the prototype model for human inflammatory demyelinating diseases of the CNS such as multiple sclerosis (MS). As endothelial cells actively participate in the regulation of lymphocyte entry into various tissues, it is likely that the specialization of the BBB endothelium extends to CNS-specific traffic signals involved in lymphocyte recruitment. Identification of the traffic signals mediating entry of lymphocytes into the CNS is likely to be of great clinical importance, as blocking the responsible molecules possibly offers new specific routes of treatment of CNS inflammatory diseases. Such a therapeutic regime, however, is not yet available. Rather, acute MS is often treated with high dose glucocorticoids. Current evidence for a possible role of glucocorticoids in regulating the expression of adhesion molecules and thus inhibiting T cell recruitment across the BBB is discussed.
    Notes: Zusammenfassung Bis vor kurzem galt das Zentralnervensystem (ZNS) als ein immunprivilegiertes Organ, d. h., ein Organ das keiner Immunüberwachung unterliegt. Man ging davon aus, dass die hochspezialisierte endotheliale Blut-Hirn-Schranke (BHS) eine unüberwindbare Barriere für zirkulierende Lymphozyten darstellt. Während entzündlicher Prozesse im ZNS erhalten jedoch zahlreiche mononukleäre Zellen Zugang zum ZNS Parenchym. Die molekularen Mechanismen der Rekrutierung von Lymphozyten durch die BHS wurden bislang vorwiegend im Tiermodell der experimentellen autoimmunen Enzephalomyelitis (EAE) studiert. Die EAE ist das beste derzeit verfügbare Modell für humane entzündliche demyelinisierende Erkrankungen des ZNS wie der Multiplen Sklerose (MS). Da Endothelzellen aktiv den Eintritt von Lymphozyten in Organe regulieren, kann man davon ausgehen, dass das spezialisierte BHS-Endothel spezifische Rezeptoren, die als “Verkehrssignale” für zirkulierende Leukozyten dienen, exprimiert. Die Identifizierung dieser BHS-spezifischen “Verkehrssignale” ist von großer klinischer Bedeutung, da ihre Blockade eine selektive Inhibition einer im ZNS ablaufenden Entzündungsreaktion erlauben würde. Eine solche Therapie steht zur Zeit noch nicht zur Verfügung. Vielmehr wird die akute MS mit hochdosierten Glukokortikoiden therapiert. Die bislang vorliegenden Evidenzen, dass der Erfolg der Glukokortikoidtherapie auf der Inhibition der Expression von Adhäsionsmolekülen und somit der Inhibition der Lymphozyten-Rekrutierung durch die BHS beruht, wird diskutiert.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    ISSN: 1432-0878
    Keywords: Key words Choroid plexus ; ICAM-1 ; VCAM-1 ; MAdCAM-1 ; Experimental autoimmune ; Encephalomyelitis ; Mouse (SJL/N)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The functional expression of vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1) and MAdCAM-1 in the choroid plexus is indicative of a role of this structure in the communication of the immune system with the central nervous system (CNS). In order to gain further insight into the possible functions of adhesion molecules expressed in the choroid plexus, we investigated the exact ultrastructural localization of VCAM-1, ICAM-1 and MAdCAM-1 on semithin and ultrathin cryosections of the choroid plexus of healthy mice and of mice suffering from experimental autoimmune encephalomyelitis (EAE). In the healthy choroid plexus VCAM-1 and ICAM-1, but not MAdCAM-1, could be detected on the apical surface of the choroid plexus epithelial cells. During EAE, immunoreactivity for VCAM-1 and ICAM-1 was dramatically increased. Additionally, apical expression of MAdCAM-1 was observed on individual choroid plexus epithelial cells during EAE. At the same time, VCAM-1, ICAM-1 or MAdCAM-1 were never present on the endothelial cells of the fenestrated capillaries within the choroid plexus. The polar expression of VCAM-1, ICAM-1 and MAdCAM-1 on the apical surface of choroid plexus epithelial cells, which form the blood-cerebrospinal fluid barrier, implies a previously unappreciated function of this barrier in the immunosurveillance of the CNS.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...