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  • 1
    ISSN: 1615-6102
    Keywords: Cell cycle ; Nutrient status ; Plant tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Patterns of cell cycle arrest or temporal modification have been investigated using suspension cultures ofAcer pseudoplatanus under nutrient limiting and nutrient starvation conditions. The results of nitrogen, phosphorous and carbohydrate starvation have been compared and contrasted with reference to the Principal Control Point hypothesis ofVan't Hof andKovacs (1972). Whilst cells suffering phosphorus or carbohydrate starvation arrest in the G 1 and G 2 phases in the approximate ratio of 4 to 1, nitrogen starved cells accumulate virtually exclusively in G 1.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1615-6102
    Keywords: Auxin ; Cell cycle ; Hormone action ; Plant tissue culture ; Synchrony
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When suspension-cultured sycamore (Acer pseudoplatanus L.) cells are deprived of the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) cell division activity ceases after a brief period of exponential growth. Feulgen microdensitometry analyses showed that sycamore cultures starved of 2,4-D do not exhibit exclusive arrest in any particular cell cycle compartment. Regrowth, following readdition of 2,4-D to 2,4-D-starved cells, produced periodic oscillations in mitotic index and discontinuous increases in cell number. Detailed characteristics of the regrowth kinetics indicated involvement of periodic, rather than synchronous, growth. The results of this study suggest that the stimulation of cell division by 2,4-D is indirect and not due to the activation of a cell cycle control point to commit cells to division.
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  • 3
    ISSN: 1615-6102
    Keywords: Cell cycle ; Cytokinin ; Hormone action ; Plant tissue culture ; Synchrony
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of 6-benzylaminopurine (BAP) withdrawal and re-application on a cytokinin-dependent suspension culture of soybean, have been examined. Data obtained from a detailed analysis of mitotic activity, cell number fluctuation and cellular DNA content, support the conclusion that, in this tissue, cytokinin deprivation does not cause arrest at any particular point in the cell cycle. Results presented here, and in the previous paper (Everett et al.), emphasize the importance in analyses of cell population kinetics of distinguishing between true cell cycle synchrony, periodic division and growth, and mitotic oscillation.
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Company
    Nature biotechnology 5 (1987), S. 1201-1204 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] A disarmed derivative of the Ti:R772 cointegrate plasmid pAL969 was used to produce transgenic plants of a commercially important inbred line of oil–producing sunflower. A chimeric gene (neo) composed of the coding sequence of neomycin phosphotransferase (NPT) and 5′ and 3′ ...
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