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  • 1
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  16. Deutscher Kongress für Versorgungsforschung (DKVF); 20171004-20171006; Berlin; DOCV020 /20170926/
    Publication Date: 2017-09-26
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 2
    Keywords: EXPRESSION ; TOLERANCE ; CAENORHABDITIS-ELEGANS ; ARABIDOPSIS-THALIANA ; SUPEROXIDE-DISMUTASE ; RICHTERSIUS-CORONIFER ; LIFE-SPAN REGULATION ; VITELLOGENIN GENES ; YOLK PROTEINS ; WATER-STRESS
    Abstract: Tardigrades have fascinated researchers for more than 300 years because of their extraordinary capability to undergo cryptobiosis and survive extreme environmental conditions. However, the survival mechanisms of tardigrades are still poorly understood mainly due to the absence of detailed knowledge about the proteome and genome of these organisms. Our study was intended to provide a basis for the functional characterization of expressed proteins in different states of tardigrades. High-throughput, high-accuracy proteomics in combination with a newly developed tardigrade specific protein database resulted in the identification of more than 3000 proteins in three different states: early embryonic state and adult animals in active and anhydrobiotic state. This comprehensive proteome resource includes protein families such as chaperones, antioxidants, ribosomal proteins, cytoskeletal proteins, transporters, protein channels, nutrient reservoirs, and developmental proteins. A comparative analysis of protein families in the different states was performed by calculating the exponentially modified protein abundance index which classifies proteins in major and minor components. This is the first step to analyzing the proteins involved in early embryonic development, and furthermore proteins which might play an important role in the transition into the anhydrobiotic state.
    Type of Publication: Journal article published
    PubMed ID: 23029181
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  • 3
    Keywords: IONIZING-RADIATION ; Germany ; MODEL ; INFORMATION ; GENE ; GENES ; DNA ; TOLERANCE ; SEQUENCE ; SEQUENCES ; WATER ; IDENTIFICATION ; CHROMATIN ; HEAT-SHOCK ; STRESS ; genetics ; DAMAGE ; DATABASE ; CAENORHABDITIS-ELEGANS ; assembly ; TRANSLATION ; EXPRESSED SEQUENCE TAGS ; transcriptome ; CONTROLLED TUMOR PROTEIN ; radiation tolerance ; Genetic ; MILNESIUM-TARDIGRADUM ; RICHTERSIUS-CORONIFER ; ADORYBIOTUS-CORONIFER ; DESICCATION TOLERANCE ; Species ; CONTRIBUTE ; EST ; ACID-BINDING PROTEINS ; FREEZE TOLERANCE ; POLYPEDILUM-VANDERPLANKI ; Sequence information
    Abstract: Background: The phenomenon of desiccation tolerance, also called anhydrobiosis, involves the ability of an organism to survive the loss of almost all cellular water without sustaining irreversible damage. Although there are several physiological, morphological and ecological studies on tardigrades, only limited DNA sequence information is available. Therefore, we explored the transcriptome in the active and anhydrobiotic state of the tardigrade Milnesium tardigradum which has extraordinary tolerance to desiccation and freezing. In this study, we present the first overview of the transcriptome of M. tardigradum and its response to desiccation and discuss potential parallels to stress responses in other organisms. Results: We sequenced a total of 9984 expressed sequence tags (ESTs) from two cDNA libraries from the eutardigrade M. tardigradum in its active and inactive, anhydrobiotic (tun) stage. Assembly of these ESTs resulted in 3283 putative unique transcripts, whereof similar to 50% showed significant sequence similarity to known genes. The resulting unigenes were functionally annotated using the Gene Ontology (GO) vocabulary. A GO term enrichment analysis revealed several GOs that were significantly underrepresented in the inactive stage. Furthermore we compared the putative unigenes of M. tardigradum with ESTs from two other eutardigrade species that are available from public sequence databases, namely Richtersius coronifer and Hypsibius dujardini. The processed sequences of the three tardigrade species revealed similar functional content and the M. tardigradum dataset contained additional sequences from tardigrades not present in the other two. Conclusions: This study describes novel sequence data from the tardigrade M. tardigradum, which significantly contributes to the available tardigrade sequence data and will help to establish this extraordinary tardigrade as a model for studying anhydrobiosis. Functional comparison of active and anhydrobiotic tardigrades revealed a differential distribution of Gene Ontology terms associated with chromatin structure and the translation machinery, which are underrepresented in the inactive animals. These findings imply a widespread metabolic response of the animals on dehydration. The collective tardigrade transcriptome data will serve as a reference for further studies and support the identification and characterization of genes involved in the anhydrobiotic response
    Type of Publication: Journal article published
    PubMed ID: 20226016
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  • 4
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  60. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit den Benelux-Ländern und Bulgarien; 20090524-20090527; Münster; DOCMI.10-06 /20090520/
    Publication Date: 2009-06-30
    Keywords: ddc: 610
    Language: English
    Type: conferenceObject
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  • 5
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  17. Deutscher Kongress für Versorgungsforschung (DKVF); 20181010-20181012; Berlin; DOC18dkvf132 /20181012/
    Publication Date: 2018-10-13
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 6
  • 7
    Keywords: EXPRESSION ; Germany ; MODEL ; INFORMATION ; SYSTEM ; GENE ; GENE-EXPRESSION ; GENOME ; PROTEIN ; PROTEINS ; RESOLUTION ; MECHANISM ; FAMILY ; DOMAIN ; mechanisms ; TOLERANCE ; CYCLE ; SEQUENCE ; IDENTIFICATION ; gene expression ; HEAT-SHOCK ; mass spectrometry ; SPECTROMETRY ; DATABASE ; MASS-SPECTROMETRY ; PROJECT ; PROTEOMICS ; PROTEIN IDENTIFICATION ; ARABIDOPSIS-THALIANA ; HIGH-RESOLUTION ; ANNOTATION ; SCIENCE ; LIFE ; MOLECULAR-MECHANISMS ; GLUTATHIONE S-TRANSFERASES ; Genetic ; protein extraction ; MILNESIUM-TARDIGRADUM ; RICHTERSIUS-CORONIFER ; ARTEMIA-FRANCISCANA ; DESICCATION TOLERANCE ; EST ; Sequence information ; Molecular mechanisms ; BRINE SHRIMP ; TREHALOSE
    Abstract: Background: Tardigrades are small, multicellular invertebrates which are able to survive times of unfavourable environmental conditions using their well-known capability to undergo cryptobiosis at any stage of their life cycle. Milnesium tardigradum has become a powerful model system for the analysis of cryptobiosis. While some genetic information is already available for Milnesium tardigradum the proteome is still to be discovered. Principal Findings: Here we present to the best of our knowledge the first comprehensive study of Milnesium tardigradum on the protein level. To establish a proteome reference map we developed optimized protocols for protein extraction from tardigrades in the active state and for separation of proteins by high resolution two-dimensional gel electrophoresis. Since only limited sequence information of M. tardigradum on the genome and gene expression level is available to date in public databases we initiated in parallel a tardigrade EST sequencing project to allow for protein identification by electrospray ionization tandem mass spectrometry. 271 out of 606 analyzed protein spots could be identified by searching against the publicly available NCBInr database as well as our newly established tardigrade protein database corresponding to 144 unique proteins. Another 150 spots could be identified in the tardigrade clustered EST database corresponding to 36 unique contigs and ESTs. Proteins with annotated function were further categorized in more detail by their molecular function, biological process and cellular component. For the proteins of unknown function more information could be obtained by performing a protein domain annotation analysis. Our results include proteins like protein member of different heat shock protein families and LEA group 3, which might play important roles in surviving extreme conditions. Conclusions: The proteome reference map of Milnesium tardigradum provides the basis for further studies in order to identify and characterize the biochemical mechanisms of tolerance to extreme desiccation. The optimized proteomics workflow will enable application of sensitive quantification techniques to detect differences in protein expression, which are characteristic of the active and anhydrobiotic states of tardigrades
    Type of Publication: Journal article published
    PubMed ID: 20224743
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  • 8
    ISSN: 1432-069X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Der Einfluß einer bilanzierten, fettfreien, synthetischen Diät (Vivasorb®) auf die Hautoberflächenlipide (HOL), sog. „casual level“, auf die Serumcholesterin- und die Serumtriglyceridwerte wurde untersucht. In der ersten Hälfte der Testperiode (10 Tage) bestanden die Kohlenhydrate ausschließlich aus Glucose oder Glucose-Oligosacchariden. In der zweiten Hälfte der Testperiode (10 Tage) wurde ein Teil der Glucose-Kohlenhydrate gegen Fructose ausgetauscht. Die Erhöhung der HOL und das Absinken der Serumcholesterinwerte in der ersten Hälfte der Diätperiode kann auf eine vermehrte Insulinsekretion (Glucose und Aminosäuren) der Bauchspeicheldrüse zurückgeführt werden. Der Abfall der HOL in der zweiten Hälfte der Diätperiode könnte auf eine verminderte Insulinsekretion zurückzuführen sein, da Fructose im Gegensatz zu Glucose die Insulinsekretion nicht stimuliert. Der Austausch von Glucose gegen Fructose zeigte keinen Einfluß auf die Serumcholesterinkonzentration.
    Notes: Summary The casual level of skin surface lipids, serum cholesterol, and serum triglycerides were studied under the influence of a balanced, fatless, synthetic diet (Vivasorb®). In the first half of the testperiod (10 days) carbohydrates consisted of glucose or glucose-oligosaccharides exclusively. A part of the glucose-carbohydrates was changed into fructose in the second half of the testperiod (10 days). The increase of skin surface lipids and the decrease of serum cholesterol in the first half of the test period may be due to an increased insulin secretion (glucose and aminoacids) of the pancreas. As fructose in contrast to glucose does not stimulate insulin secretion the decrease of skin surface lipids in the second half of the test period could be attributed to a diminished insulin secretion. Serum cholesterol was not affected by the exchange from glucose to fructose.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Archives of dermatological research 250 (1974), S. 229-235 
    ISSN: 1432-069X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Psoriasisschuppen wurden homogenisiert, fraktioniert zentrif ugiert und die p-Nitrophenyl-Phosphat-Phosphatase des 15000 × g Überstandes durch Affinitätschromatographie angereichert. Der Reinigungsfaktor wurde mit 7,4 bestimmt. Der Einfluß verschiedener Säureanionen und mehrerer Metallionen auf die angereicherte Enzymaktivität wurde untersucht und mit anderen sauren Phosphatasen verglichen. Das Enzym der Psoriasisschuppen konnte durch Fluorid, Tartrat, p-Chlormercuribenzoat, Be2+, Fe3+, Cu2+, Zn2+ und Pb2+ gehemmt werden. Die Michaelis-Konstante der angereicherten sauren Phosphatase des 15000 × g Überstandes (vereinigte lösliche und ribosomale Fraktion) wurde mit 1,42 × 10−2 M berechnet. Ein Vergleich mit der Michaelis-Konstanten des ebenfalls angereicherten Enzyms des 105000 × g Überstandes (cytoplasmatische Fraktion) aus Psoriasisschuppen (1,67 × 10−3 M) ergab eine deutlich höhere Affinität der cytoplasmatischen sauren Phosphatase.
    Notes: Summary Psoriatic scales were homogenized and a differential centrifugation was performed. The 15000 × g supernatant acid p-nitrophenyl phosphate phosphatase was enriched by substrate affinity chromatography. The purification factor was calculated to 7.4. The influence of several acid radicals and metal ions was tested on the partially purified enzyme activity and compared to other acid phosphatases. The psoriatic enzyme could be inhibited by fluoride, tartrate, p-chloromercuribenzoate, be2+, Fe3+, Cu2+, Zn2+, and Pb2+. The Michaelis constant of the partially purified acid phosphatase of the 15000 × g supernatant (combined soluble and ribosomal fraction) was calculated to 1.42 × 10−2 M. A comparison with the Michaelis constant of the likewise enriched enzyme of the 105000 × g supernatant (cytoplasmatic fraction) from psoriatic scales (1.67 × 10−3 M) yielded a distinct higher affinity of the cytoplasmatic acid phosphatase.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-069X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Zwei in wasserlöslichen Psoriasisschuppenextrakten nach disc-elektrophoretischer Trennung verstärkt auftretende Proteinbanden wurden isoliert und ihre Aminosäurenzusammensetzung bestimmt. Es zeigte sich, daß die Bande 9 reich an Glycin, Alanin, Asparaginsäure, Leucin und Serin und die Bande 10 reich an Glycin, Threonin und Serin ist. Beide Proteine enthalten einen Aminozuckeranteil (Galaktosamin).
    Notes: Summary Two protein bands of water soluble extracts of psoriatic scales, which appear intensified in disc-electrophoretical separation, were isolated and their amino acid compositions were determined. It could be demonstrated that band 9 was rich of glycine, alanine, aspartic acid, leucine, and serine and that band 10 was rich of glycine, threonine, and serine. Both proteins contained an amino-sugar (galactosamine).
    Type of Medium: Electronic Resource
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