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  • 1
    ISSN: 1617-4623
    Keywords: Key words Filamentous fungi ; DNA transposon ; Fot1-like element ; Podospora anserina ; Repeat-induced point mutation (RIP)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A degenerate DNA transposon, Pat, was identified in the genomes of various wild-type strains of the filamentous fungus Podospora anserina. In these strains, the number (approximately 20–25 copies per genome) and location of Pat sequences appear to be conserved. Two copies of Pat, one complete and one partial, were cloned and characterized. The sequence of the complete element is 1856 bp long and contains imperfect inverted terminal repeats (ITRs) of 53 bp. The target site duplication comprises the sequence TA. The amino acid sequence derived from one reading frame of Pat shows significant homology to members of the Fot1 family of transposons. However, this reading frame is interrupted by numerous stop codons. Since no transcripts of Pat were identified in different P. anserina strains grown under standard conditions and under increased stress, we conclude that none of the copies of Pat is active in the strains analyzed, under the environmental conditions investigated. Comparison of the sequences of the two cloned Pat sequences revealed 89% (589/747 nucleotides) identity. Most of the differences (82%, 129/158) can be attributed to transitions preferentially at CpA:TpG and CpT:ApG dinucleotides. The dinucleotide ratios in Pat are similar to those in a Neurospora crassa transposon which was subject to repeat-induced mutation (RIP), but differ significantly from those found in single-copy genes of P. anserina and in fungal DNA transposons not modified by this mechanism. Molecular analysis of the progeny of a cross between the wild-type strain and a transgenic strain in which a nuclear gene was duplicated by transformation yielded the first clear evidence that a RIP-like process is active in P. anserina.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 79 (2001), S. 779-781 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We demonstrate the pyroelectric effect in a conjugated polymer, poly(2,5-pyridinediyl) (PPY), and we use the laser intensity modulation method (LIMM) to resolve the spatial distribution of electric field and space charges inside a 5 μm Au/PPY/Au sandwich device. The pyroelectric signal shows hysteresis behavior with respect to the applied bias indicating permanent storage of injected charges. From the analysis of the LIMM spectra we conclude that application of a bias leads to the accumulation of space charges near the electrodes, while a zone of opposite space charge may establish in a distance of about 1 μm from it. The charged state retains after removing the bias and can lead to an internal electric field that is opposite to the external poling field in the bulk of the polymer film. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 76 (2000), S. 664-666 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Photocurrent measurements have been carried out on thin films of poly(2,5-pyridinediyl), a promising polymer for applications in light-emitting diodes. The spectral dependence of the photocurrent has been recorded using the four possible directions of the applied electric field and illumination, and clear symbatic and antibatic behavior was observed. A strong, symmetric photocurrent increase with bias has been found, while the temperature dependence is relatively weak. The analysis of the results provides evidence that electrons are the majority charge carriers and gives insight into the carrier-transport mechanism in poly(2,5-pyridinediyl). © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
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