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  • 1
    Keywords: Biochemistry ; Pharmaceutical technology ; Biochemistry, general ; Pharmaceutical Sciences/Technology ; Springer eBooks
    Description / Table of Contents: Molecular Evolution Bioinformatics towards Structural Biology of TRPV1-4 Channels -- Expression, Purification, and Crystallization of the Transient Receptor Potential Channel TRPV6 -- Cryo-Electron Microscopy of TRP Channels -- Mass Spectrometry-Based Analysis of TRPP2 Phosphorylation -- Combining Structural Data with Computational Methodologies to Investigate Structure-Function Relationships in TRP Channels -- Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique -- Fluorescence-Based Functional Assays for Ca2+ Permeable ThermoTRP Channels -- High Content Imaging of Immunofluorescently-Labeled TRPV1-Positive Sensory Neurons -- Patch-Clamp Combined with Fast Temperature Jumps to Study Thermal TRP Channels -- TRP Channels Reconstitution in Lipid Bilayers -- Methods for Investigating TRP Channel Gating -- In Silico Approaches for TRP Channel Modulation -- Drug Discovery for Soft Drugs on TRPV1 and TRPM8 Channels Using the Passerini Reaction -- In Vivo Methods to Study ThermoTRP Channels in Rodents
    Abstract: This book aims to provide a guide to researchers on how to work with Transient Receptor Potential (TRP) channels, describing current methodologies and protocols, and highlighting the challenges ahead that will require the development of new technologies. Articles cover a wide range of techniques, from structure to function, concluding with how to best to evaluate some of the in vivo function of TRP channels, particularly in regard to their involvement in hyperalgesia and allodynia. Also, the collection describes important and inspiring available tools that are currently being used to investigate the activity of these channels in vitro and in vivo in terms of their underlying protein structure, segueing into dealing with the generation of pharmacological tools to explore their in vivo activity and their involvement in cellular signaling pathways, which may be developed into drugs to treat diseases caused by channel dysfunction or hyperactivity. Written for the highly successful Methods in Molecular Biology series, chapters include the necessary detail and implementation advice to ensure successful results in the lab. Authoritative and practical, TRP Channels: Methods and Protocols is a useful set of methodologies for scientists seeking to better understand this vital family of channels
    Pages: X, 238 p. 69 illus., 58 illus. in color. : online resource.
    ISBN: 9781493994465
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Food Chemistry 51 (1994), S. 153-157 
    ISSN: 0308-8146
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0428
    Keywords: Keywords Glycated haemoglobin, endothelial dysfunction, nitric oxide, superoxide anions, antioxidants, human microvessels.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Aims/hypothesis. It has been recently shown that glycated human haemoglobin induces endothelial dysfunction in rat vessels by generating superoxide anions that interfere with nitric oxide mediated responses. Our study analysed the effect of glycated human haemoglobin on the endothelium-dependent relaxations of human vessels.¶Methods. Omental microvessels were obtained from patients (without diabetes, hypertension or vascular disease) during surgery and mounted in a small vessel myograph to study their vasoactive responses (vessels from 3–7 patients for each set of experiments).¶Results. Cumulative vasodilatory responses to bradykinin (10 nmol/l to 3 μmol/l) were induced in vessels precontracted with 35–50 mmol/l potassium chloride. Addition of 100 μmol/l N G-nitro-l-arginine methyl ester reduced the relaxation evoked by bradykinin, but preincubation with both N G-nitro-l-arginine methyl ester and 10 μmol/l indomethacin was needed to abolish it. Bradykinin-induced responses were inhibited by 1 μmol/l non–glycated oxyhaemoglobin whereas no effect was obtained with 10 nmol/l oxyhaemoglobin. At these low concentrations (10 nmol/l), glycated human oxyhaemoglobin caused an impairment of bradykinin-induced relaxation when the percentage of glycation was 10 % or higher. This effect was prevented by preincubating the vessels with ascorbic acid (10 μmol/l), superoxide dismutase (100 U/ml) and gliclazide (1 and 10 μmol/l), but not with indomethacin (10 μmol/l), catalase (400–600 U/ml), dimethylthiourea (1 mmol/l) or glibenclamide (10 μmol/l). In vessels preincubated with N G-nitro-l-arginine methyl ester (100 μmol/l), glycohaemoglobin did not add any additional effect.¶Conclusion/interpretation. Highly glycated human oxyhaemoglobin, at physiological plasmatic concentrations, impairs nitric oxide-mediated responses by a mechanism involving superoxide anions but not cyclooxygenase derivatives. [Diabetologia (2000) 43: 83–90].
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0942-0940
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A 49-year-old woman had a left frontal expanding mass with calcified areas. A craniotomy and resection of the mass were performed. Histological sections showed a mixed tumour: osteochondrosarcoma-glioblastoma multiforme. The absence of previous irradiation and its location, deep in the frontal lobe and apparently unattached to the meninges, add to the rarity of this association. A brief discussion concerning the presence of osseous or cartilaginous areas in brain tumours and the problem of cerebral mixed tumours follows the description of the case.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Phytochemistry 22 (1983), S. 813-816 
    ISSN: 0031-9422
    Keywords: Leguminosae ; Voandzeia subterranea ; bambara pea ; plant tissues. ; protein ; trypsin-like inhibitor
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Phytochemistry 22 (1983), S. 813-816 
    ISSN: 0031-9422
    Keywords: Leguminosae ; Voandzeia subterranea ; bambara pea ; plant tissues ; protein ; trypsin-like inhibitor
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 7
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  57th Annual Meeting of the German Society for Neuropathology and Neuroanatomy (DGNN); 20120912-20120915; Erlangen; DOC12dgnnPP4.17 /20120911/
    Publication Date: 2012-09-12
    Keywords: ddc: 610
    Language: English
    Type: conferenceObject
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  • 8
    Abstract: Common genetic variants contribute to the observed variation in breast cancer risk for BRCA2 mutation carriers; those known to date have all been found through population-based genome-wide association studies (GWAS). To comprehensively identify breast cancer risk modifying loci for BRCA2 mutation carriers, we conducted a deep replication of an ongoing GWAS discovery study. Using the ranked P-values of the breast cancer associations with the imputed genotype of 1.4 M SNPs, 19,029 SNPs were selected and designed for inclusion on a custom Illumina array that included a total of 211,155 SNPs as part of a multi-consortial project. DNA samples from 3,881 breast cancer affected and 4,330 unaffected BRCA2 mutation carriers from 47 studies belonging to the Consortium of Investigators of Modifiers of BRCA1/2 were genotyped and available for analysis. We replicated previously reported breast cancer susceptibility alleles in these BRCA2 mutation carriers and for several regions (including FGFR2, MAP3K1, CDKN2A/B, and PTHLH) identified SNPs that have stronger evidence of association than those previously published. We also identified a novel susceptibility allele at 6p24 that was inversely associated with risk in BRCA2 mutation carriers (rs9348512; per allele HR = 0.85, 95% CI 0.80-0.90, P = 3.9 x 10(-8)). This SNP was not associated with breast cancer risk either in the general population or in BRCA1 mutation carriers. The locus lies within a region containing TFAP2A, which encodes a transcriptional activation protein that interacts with several tumor suppressor genes. This report identifies the first breast cancer risk locus specific to a BRCA2 mutation background. This comprehensive update of novel and previously reported breast cancer susceptibility loci contributes to the establishment of a panel of SNPs that modify breast cancer risk in BRCA2 mutation carriers. This panel may have clinical utility for women with BRCA2 mutations weighing options for medical prevention of breast cancer.
    Type of Publication: Journal article published
    PubMed ID: 23544012
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  • 9
    Keywords: IN-VITRO ; HEPATOCELLULAR-CARCINOMA ; MICE ; STEM-CELLS ; MOUSE-LIVER ; OVAL CELLS ; hepatic progenitor cells ; BILIARY EPITHELIAL-CELLS ; MATURE HEPATOCYTES ; ADULT HEPATOCYTES
    Abstract: Ductular reactions (DRs) are observed in virtually all forms of human liver disease; however, the histogenesis and function of DRs in liver injury are not entirely understood. It is widely believed that DRs contain bipotential liver progenitor cells (LPCs) that serve as an emergency cell pool to regenerate both cholangiocytes and hepatocytes and may eventually give rise to hepatocellular carcinoma (HCC). Here, we used a murine model that allows highly efficient and specific lineage labeling of the biliary compartment to analyze the histogenesis of DRs and their potential contribution to liver regeneration and carcinogenesis. In multiple experimental and genetic liver injury models, biliary cells were the predominant precursors of DRs but lacked substantial capacity to produce new hepatocytes, even when liver injuries were prolonged up to 12 months. Genetic modulation of NOTCH and/or WNT/beta-catenin signaling within lineage-tagged DRs impaired DR expansion but failed to redirect DRs from biliary differentiation toward the hepatocyte lineage. Further, lineage-labeled DRs did not produce tumors in genetic and chemical HCC mouse models. In summary, we found no evidence in our system to support mouse biliary-derived DRs as an LPC pool to replenish hepatocytes in a quantitatively relevant way in injury or evidence that DRs give rise to HCCs.
    Type of Publication: Journal article published
    PubMed ID: 25915586
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  • 10
    Keywords: CANCER ; CELL ; LUNG ; LUNG-CANCER ; RISK ; GENE ; PROTEIN ; ASSOCIATION ; SUSCEPTIBILITY LOCUS ; VARIANTS ; BREAST-CANCER ; genetics ; COLORECTAL-CANCER ; PROSTATE-CANCER ; MELANOMA ; VARIANT ; telomere length ; RISK-FACTOR ; BASAL-CELL CARCINOMA ; GENOME-WIDE ASSOCIATION ; SCAN ; COMMON VARIANTS
    Abstract: The common sequence variants that have recently been associated with cancer risk are particular to a single cancer type or at most two. Following up on our genome-wide scan of basal cell carcinoma(1), we found that rs401681[C] on chromosome 5p15.33 satisfied our threshold for genome-wide significance (OR 1.25, P = 3.7 x 10(-12)). We tested rs401681 for association with 16 additional cancer types in over 30,000 cancer cases and 45,000 controls and found association with lung cancer (OR = 1.15, P = 7.2 x 10(-8)) and urinary bladder, prostate and cervix cancer (ORs = 1.07-1.31, all P 〈 4 x 10(-4)). However, rs401681[C] seems to confer protection against cutaneous melanoma (OR = 0.88, P = 8.0 x 10(-4)). Notably, most of these cancer types have a strong environmental component to their risk. Investigation of the region led us to rs2736098[A], which showed stronger association with some cancer types. However, neither variant could fully account for the association of the other. rs2736098 corresponds to A305A in the telomerase reverse transcriptase (TERT) protein and rs401681 is in an intron of the CLPTM1L gene
    Type of Publication: Journal article published
    PubMed ID: 19151717
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