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  • 1
    ISSN: 1432-2242
    Keywords: Restriction fragment length polymorphism ; CCN ; Genetic mapping ; Triticum aestivum ; Heterodera avenae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cereal cyst nematode (CCN) (Heterodera avenae Woll.) is an economically damaging pest of wheat in many of the worlds cereal growing areas. The development of CCN-resistant cultivars may be accelerated by the use of molecular markers. The Cre gene of the wheat line “AUS 10894” confers resistance to CCN. Using a pair of near-isogenic lines (NILs) that should differ only in a small chromosome segment containing the Cre locus, we screened 58 group-2 probes and found two (Tag605 and CDO588) that detect polymorphism between the NILs. Nulli-tetrasomic and ditelosomic lines confirmed that the restriction fragment length polymorphism (RFLP) markers identified were derived from the long arm of wheat chromosome 2. Crosses between “AUS 10894” and “Spear” and the NIL “AP” and its recurrent parent “Prins” were used to produce F2 populations that gave the expected 3∶1 segregation ratio for the resistance gene. Linkage analysis identified two RFLP markers flanking the resistance gene. Xglk605 and Xcdo588 mapped 7.3 cM (LOD=6.0) and 8.4 cM (LOD=6.7), respectively, from the Cre locus.
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  • 2
    ISSN: 1432-2242
    Keywords: Heterodera avenae ; Resistance ; Isozymes ; C-banding ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cereal cyst nematode,Heterodera avenue Wollenweber, is a serious pest of cereals in many countries. A high level of resistance to the unique Australian pathotype of the nematode has been demonstrated in a triticale line (T701-4-6), which was originally obtained from CIMMYT. The level of resistance is similar to that in rye cultivar, South Australian, but higher than that in the wheat line (AUS 10894), hitherto reported to have useful resistance to the Australian pathotype. The gene for resistance was located on rye chromosome 6 (6R) after backcrossing the T701-4-6 line to wheat and correlating the resistance with the presence of individual rye chromosomes identified by morphological, cytological, and isozyme markers. Preliminary evidence suggests that the gene is located on the long arm of6R. To transfer the resistance to wheat, double monosomics of6R and6D in aph1bph1b homozygous background were selected from F2 progeny from a cross of disomic6R substitution for6D to theph1b mutant. Selfed seeds from these F2 plants will be screened for wheat-rye chromosome recombinants.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1420-9071
    Keywords: Lymphoma, primary culture ; copper chelator ; copper, endogenous ; copper, cytotoxicity ; bathocuproine sulfonate ; cysteine oxidation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary With the use of bathocuproine sulfonate, a copper-specific chelator as an indicator, we have demonstrated that copper ions, present as a natural medium constituent are toxic to the growth of a lymphoma in primary culture and are principally responsible for the growth requirement of mercaptoethanol and other thiols. By chelating trace copper normally present in the medium, bathocuproine sulfonate retarded the oxidation of cysteine to poorly utilized cystine, thus permitting its direct utilization by the cells for growth.
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 223 (1969), S. 944-946 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The present experiments attempt to demonstrate the presence of mitotic inhibitors in the adult mammalian lung using the Colcemid metaphase-arrest technique. It has already been shown that lung maintained for 3 days in organ culture is a suitable experimental model6. Using Trowell's organ culture ...
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