Description / Table of Contents:
Advances in Research on Metalloproteins -- Purification of O2-Sensitive Metalloproteins -- Expression and Purification of NifB Protein from Aerobic and Anaerobic Sources -- Techniques for the Production, Isolation and Analysis of Iron-Sulfur Proteins -- Cell-Free Synthesis of the H-cluster: a Model for the In Vitro Assembly of Metalloprotein Metal Centers -- Electrochemistry of Metalloproteins – Protein Film Electrochemistry for the Study of E. coli [NiFe]-Hydrogenase-1 -- FTIR Spectroscopy of Metalloproteins -- Characterizing Millisecond Intermediates in Hemoproteins Using Rapid-Freeze-Quench Resonance Raman Spectroscopy -- A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and Model Compounds -- Study of Metalloproteins Using Continuous Wave Electron Paramagnetic Resonance (EPR) -- Mössbauer Spectroscopy -- X-Ray Absorption Spectroscopy of Metalloproteins -- X-Ray Crystallographic Studies of Metalloproteins -- Quantum Mechanical Methods for the Investigation of Metalloproteins and Related Bioinorganic Compounds -- Density Functional Theory – Broken Symmetry (DFT-BS) Methodology Applied to Electronic and Magnetic Properties of Bioinorganic Prosthetic Groups
Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mössbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field
X, 299 p. 89 illus., 57 illus. in color. : online resource.