Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    ISSN: 0890-8508
    Keywords: HIV ; PCR ; biotinylated DNA probe
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 0890-8508
    Keywords: Molluscum contagiosum virus ; biotinylated DNA probe ; in situ hybridization
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The presence of specific antibodies to human herpesviruses in certain purified human IgG preparations from commercial sources may produce misleading results when such preparations are used in studies on the IgG receptors produced by virus-infected cells.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In mice infected with herpes simplex virus (HSV) type 1 by the intracerebral route or with HSV type 2 by the vaginal route, virus was shown to be present in greater quantities in purified platelets than in leucocytes or other blood components. Results of the studies described suggest that platelets may play a major role in the hematogenous dissemination of HSV infection.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Glycoprotein B (gB) is an essential glycoprotein of herpes simplex virus (HSV) and a major target for cellular and humoral immune response in the infected host. In the present study, we have analyzed the pattern of reactivity of a panel of 23 HSV-seropositive patient sera using as test antigens gB derivatives made in COS cells in a transient expression assay. Our results show that nearly all the sera tested, reacted with wild-type gB or tgB (772) (that lacks 102 amino acids cytoplasmic domain). However, when tgB (477 amino acids) or gBdl (an inframe deletion between amino acids 477–772) were used as test antigens only 12 out of 23 sera tested positive. Further analysis using competition assays revealed that these sera can be classified into at least two groups: (i) that contain gB-reactive antibodies reactive to intact gB or tgB (772); (ii) that contain antibodies that recognize all forms of gB-derivatives tested. The results presented here underscore the potential limitations in using certain truncated forms of gB as antigens for subunit vaccine or in the serodiagnosis of HSV infection.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  The epitope stability of a varicella-zoster virus (VZV) glycoprotein E (gE) was analyzed with monoclonal antibodies (mAbs) in cells infected with different passages of various VZV strains and isolates. The gE-specific mAbs recognized same antigenic sites (epitopes) in VZV isolates with various passage history. All VZV strains and virus-isolates reacted with an anti-gE monoclonal antibody by immunoprecipitation, or indirect fluorescent antibody staining test. Sera from VZV seropositive individuals reacted with a truncated VZV gE glycoprotein, designated TgpI-511. Also, human mononuclear cells (MNCs) stimulated with TgpI-511 glycoprotein were shown to produce VZV-specific antibodies in vitro. The results demonstrated the stability of these gE epitopes tested in this study in TgpI-511 and among the VZV-isolates obtained from different passages. These results also suggest that VZV glycoproteins as well as live attenuated or killed varicella vaccines containing these epitopes could be used as therapeutic booster vaccines in adults and the elderly to prevent zoster.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary. The genome of varicella-zoster virus (VZV), encodes at least six glycoproteins and they elicit the formation of complement-independent, complement-dependent, and non-neutralizing antibody responses. We have used our library of MAbs to VZV glycoprotein E (gE) to determine the neutralizing epitopes of gE, and shown that gE has 3 distinct neutralizing domains. In this report we have used the baculovirus expression system to identify the antigenic domains of gE. We have generated 3 recombinant baculoviruses, expressing the full-length gE and two overlapping truncated forms (the amino-terminal and the carboxy-terminal) of gE. By immuno-fluorescence and immunoblotting we have explored the physical interactions of Mabs to gE on these constructs. Our panel of MAbs revealed 3 district antigenic domains on gE. All MAbs reacted with the full-length gE; MAbs with high titered complement-dependent neutralizing activities reacted with the N-terminal truncated gE; MAbs with low titered or non-neutralizing activities reacted with the C-terminal truncated gE; MAbs with complement-enhanced neutralizing activities reacted with both truncated constructs. However, although the antibody binding in immunofluorescence and immunoblotting was carried out under denatured conditions, whereas the neutralization is under non-denatured conditions, still the antigenic mapping was similar in both conditions.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 67 (1990), S. 5833-5833 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: In the design of many electromagnetomechanical devices one of the prime parameters of interest is the mechanical force of torque produced by the device. The generated forces are of interest in two forms. The first is the distribution, which might be used to indicate a particular loading on some part of the structure; the second is a global value which appears at the mechanical interface. It is with the calculation of the latter that this paper is concerned. Much has been published concerning force calculations in two-dimensional solutions and it is the intention of this paper to discuss the extension of these methods to three-dimensional, scalar potential based analyses and to give examples of computed and measured results on several different devices.In general, the methods that are used in two dimensions are applicable in three dimensions. These are ususally classified as single- and multiple-solution techniques. Into the first category fall Maxwell stresses and the Coulomb implementation of virtual work; into the second falls the classical virtual work approach. The Maxwell stress approach requires a surface integral of a product of flux density (B) components. In two dimensions this is a relatively simple task; it reduces to defining a line in the plane and computing B as the differential of A (the vector potential). In three dimensions, the definition of a surface may not be simple and, if a scalar potential is used for the solution, the computation of flux density components is far from trivial. When these requirements are compared with the classical virtual work approach, the reduction in computing effort by using a single-solution technique many not seem so significant. A second factor influencing the use of a multiple-solution approach is fundamental to the finite-element method; in general, the method produces accurate values for global quantities such as coenergy, with coarser discretizations than those required for local accuracy. Single-solution methods tend to require high-accuracy local fields and thus require considerably more computer effort per solution. On problems ranging from a disk drive actuator to a permanent magnet generator accuracies within 3% of measured have been computed with virtual work. The equivalent two-dimensional solutions did not do better than 10%.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...