Biochemistry and Biotechnology
Wiley InterScience Backfile Collection 1832-2000
Chemistry and Pharmacology
Protein blotting is used to identify and characterize the ligand binding site of the nicotinic acetylcholine receptor. The affinity of the complex formed on the filter can be determined by quantitative blot analyses. The precise binding-sequence can be pinpointed by toxin overlay of proteolysed receptor α-subunit. These techniques are now being employed in the analysis of bacterially expressed toxin binding sites.
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