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  • 1
    ISSN: 1432-0428
    Keywords: Glycosylated haemoglobin ; standards ; references ; microcolumn ; high pressure liquid chromatography ; thiobarbituric acid ; affinity column
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Four assays; high pressure liquid chromatography, colorimetric with thiobarbituric acid, affinity columns, and microcolumn cation exchange were compared for (1) ability to discriminate between samples taken from diabetic and normal subjects; (2) correlation with each other; (3) stability over time at different temperatures; and (4) reproducibility between laboratories. The most discriminatory (10 samples from a diabetic and 10 samples from a normal group) was the microcolumn cation exchange method (t=5.25; p〈0.001), but all were significantly different at p〈0.005. The intra-assay coefficient of variation was 1%–6%, except for the affinity column method which was 13% in normal subjects. High pressure liquid chromatography was used as a reference and the other assays correlated well (r=0.93–0.99). Storage at-80 °C, -20 °C, 4 °C, and 24 °C showed marked differences. The thiobarbituric acid method results were stable except for 24 °C. Microcolumn cation exchange was labile under all conditions. Affinity column was stable for up to 15 days, only if samples were stored as whole blood. High pressure liquid chromatography showed an increase in haemoglobin A1a+b and a decrease in the haemoglobin A1c. Haemoglobin A1c was reproducible for 4 days when stored at 4 °C and up to 11 days when stored at -80 °C. Samples exchanged between centres at 4 °C and performed within 5 days by high pressure liquid chromatography for haemoglobin A1 and haemoglobin A1c correlated well (r=0.98 and 0.99). Samples exchanged between centres after storage (up to 40 days -80°C) correlated (r=0.99) by the thiobarbituric acid method. Thus, standards can be prepared for the thiobarbituric acid method and this method with high pressure liquid chromatography could be used to establish references for clinical assays.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0428
    Keywords: Key words Follow-up study, glycated Hb, HbA1 c, IGT, Pima Indians.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Glycated haemoglobin could offer several practical advantages over the OGTT for assessing glucose metabolism. Initial cross-sectional studies (1983–1985) on 381 subjects (mostly Pima Indians) described the relationship between HbA1 c (a specific glycated Hb) and the OGTT. We performed follow-up OGTTs and HbA1 c measurements on 257 of these same subjects 1.6–6.1 years later. Subjects were again grouped according to both the result of the OGTT (normal, IGT or diabetes, by WHO criteria) and HbA1 c result (normal or elevated based on mean ±1.96 SD of normal). Of 66 subjects with IGT at baseline, 47 (71 %) had normal HbA1 c and 19 (29 %) had elevated HbA1 c. Twenty-six (39 %) of these subjects had diabetes at follow-up. Of these subjects with IGT, a significantly greater percentage of subjects with elevated HbA1 c at baseline (68 %) showed worsening to diabetes than those with a normal HbA1 c (28 %); (chi-square =7.8, df =1, p〈0.01). Thus, in subjects with IGT, glycated Hb may be a useful predictor of progression to diabetes. [Diabetologia (1994) 37: 252–256]
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0428
    Keywords: Follow-up study ; glycated Hb ; HbA1c ; IGT ; Pima Indians
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Glycated haemoglobin could offer several practical advantages over the OGTT for assessing glucose metabolism. Initial cross-sectional studies (1983–1985) on 381 subjects (mostly Pima Indians) described the relationship between HbA1c (a specific glycated Hb) and the OGTT. We performed follow-up OGTTs and HbA1c measurements on 257 of these same subjects 1.6–6.1 years later. Subjects were again grouped according to both the result of the OGTT (normal, IGT or diabetes, by WHO criteria) and HbA1c result (normal or elevated based on mean ± 1.96 SD of normal). Of 66 subjects with IGT at baseline, 47 (71%) had normal HbA1c and 19 (29%) had elevated HbA1c. Twentysix (39%) of these subjects had diabetes at follow-up. Of these subjects with IGT, a significantly greater percentage of subjects with elevated HbA1c at baseline (68%) showed worsening to diabetes than those with a normal HbA1c (28%); (chi-square=7.8, df=1, p〈0.01). Thus, in subjects with IGT, glycated Hb may be a useful predictor of progression to diabetes.
    Type of Medium: Electronic Resource
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