T and innate lymphoid cells (ILCs) share some aspects of their developmental programs. However, although Notch signaling is strictly required for T cell development, it is dispensable for fetal ILC development. Constitutive activation of Notch signaling, at the common lymphoid progenitor stage, drives T cell development and abrogates ILC development by preventing Id2 expression. By combining single-cell transcriptomics and clonal culture strategies, we characterize two heterogeneous alpha4beta7-expressing lymphoid progenitor compartments. alphaLP1 (Flt3(+)) still retains T cell potential and comprises the global ILC progenitor, while alphaLP2 (Flt3(-)) consists of ILC precursors that are primed toward the different ILC lineages. Only a subset of alphaLP2 precursors is sensitive to Notch signaling required for their proliferation. Our study identifies, in a refined manner, the diversity of transitional stages of ILC development, their transcriptional signatures, and their differential dependence on Notch signaling.
Type of Publication:
Journal article published