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  • 1
    Keywords: Biological models ; Proteomics ; Gene Expression ; Systems Biology ; Proteomics ; Gene Expression ; Springer eBooks
    Description / Table of Contents: 1. The Potential of €˜Ómics to Link Lipid Metabolism and Genetic and Comorbidity Risk Factors of Alzheimeŕ€™s Disease in African Americans -- 2. The Role of Biomarkers in Alzheimeŕ€™s Disease Drug Development -- 3. Mitochondrial Involvement in Mental Disorders; Energy Metabolism, Genetic and Environmental Factors -- 4. Lymphocytes, Platelets, Erythrocytes and Exosomes as Possible Biomarkers for Alzheimer's Disease Clinical Diagnosis -- 5. Genetic Risk Factors for Alzheimer Disease: Emerging Roles of Microglia in Disease Pathomechanisms -- 6. Neuroimaging Studies of Cognitive Function in Schizophrenia -- 7. The Role of Biomarkers in Psychiatry -- 8. Interactome Studies of Psychiatric Disorders -- 9. MicroRNAs in Major Depressive Disorder -- 10. Proteomic Markers for Depression -- 11. Advances in Biomarker Studies in Autism Spectrum Disorders -- 12. Proteomic Investigations of Autism Spectrum Disorder: Past Findings, Current Challenges and Future Prospects -- 13. Role of the Gut Microbiome in Autism Spectrum Disorders -- 14. Metabolomic Biomarkers in Mental Disorders: Bipolar Disorder and Schizophrenia -- 15. Early Detection and Treatment of Patients with Alzheimeŕ€™s Disease: Future Perspectives
    Abstract: This book includes a series of reviews on general aspects of biomarker use in the study of psychiatric and neurodegenerative diseases and the development of medications involved in their treatment. It describes the pros and cons of the various approaches and covers the successes and failures in this research field. It is only by a thorough understanding of the shortcomings that progress can be made. The overall goal is to facilitate the understanding and treatment of these disorders, by providing a viable mechanism of catching up with other areas of modern medicine, such as diabetes and heart disease. Finally, it is anticipated that the development and application of valid biomarker tests and the leveraging of novel drug targets will help the fields of psychiatry on neurodegenerative disorders move into the area of personalized medicine where the right patients can receive the right medication at the right time for the best possible outcome
    Pages: XIII, 328 p. 28 illus., 26 illus. in color. : online resource.
    ISBN: 9783030055424
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  • 2
    Keywords: Medicine ; Molecular Biology ; Biomedicine ; Molecular Medicine ; Springer eBooks
    Description / Table of Contents: Nutritional programming effects on development of metabolic disorders in later life -- Effects of Prenatal Nutrition and the Role of the Placenta in Health and Disease -- Developmental Origins of Stress and Psychiatric Disorders -- Proteomic Studies of Psychiatric Disorders -- Developmental Origins of Breast Cancer: A Paternal Perspective -- Point-of-care Testing and Personalized Medicine for Metabolic Disorders -- Pregnancy and Lactation a Window of Opportunity to Improve Individual Health -- Utility of Small Animal Models of Developmental Programming -- Generation of Maternal Obesity Models in Studies of Developmental Programming in Rodents -- Generation of the Maternal Low Protein Rat Model for Studies of Metabolic Disorders -- Investigation of Paternal Programming of Breast Cancer Risk in Female Offspring in Rodent Models -- Studies of Isolated Peripheral Blood Cells as a Model of Immune Dysfunction -- Studies of a Neuronal Cell Line as a Model of Psychiatric Disorders -- Assessment of Placental Transport Function in Studies of Disease Programming -- Assessment of Fatty Liver in Models of Disease Programming -- Capillary Blood Sampling from the Finger -- Physical Activity Assessment in Clinical Studies of Substance use Disorder -- Cardiopulmonary Exercise Testing -- Cardiovascular Assessment in Human Research -- Nutritional Programming Effects on the Immune System -- Small RNA Sequencing - A Technique for miRNA Profiling -- Pulse-chase Biosynthetic Radiolabelling of Pancreatic Islets to Measure Beta Cell Function -- Rapid and Easy Protocol for Quantification of Next Generation Sequencing Libraries -- Telomere length analysis - A Tool for Dissecting Ageing Mechanisms in Developmental Programming -- Qualitative and quantitative NMR Approaches in Blood Serum Lipidomics -- In vivo Electrical Stimulation for the Assessment of Skeletal Muscle Contractile Function in Murine Models -- Experimental Model of Skeletal Muscle Laceration in Rats -- Neuropsychiatric Sequelae of Early Nutritional Modifications: A Beginner’s Guide to Behavioural Analysis -- Hyperlocomotion Test for Assessing Behavioural Disorders -- 2D-DIGE Analysis of Eye Lens Proteins as a Measure of Cataract Formation -- Mass Spectrometry Profiling of Pituitary Glands -- Multiplex Immunoassay Profiling of Hormones Involved in Metabolic Regulation -- Time Resolved Fluorescence Assays for Quantification of Insulin Precursors in Plasma and Serum -- Identification of Neural Stem Cell Biomarkers by Isobaric Tagging for Relative and Absolute Quantitation (iTRAQ) Mass Spectrometry -- Lab-on-a-chip Device for Rapid Measurement of Vitamin D Levels -- Kidney Smartphone Diagnostics -- A User-friendly App for Blood Coagulation Disorders -- Hormonal Smartphone Diagnostics
    Abstract: This volume contains several reviews in the field as well as the step-by-step use of targeted and global approaches within the areas of genomics, epigenetics, proteomics, transcriptomics and metabolomics which aim to address this dilemma and to help pinpoint new treatment strategies. Chapters detail the generation of several models and methods for assessing health and provide researchers potential approaches for reversing or minimizing effects of disease. In addition, important information on disease mechanisms is provided, as each method is described in the context of a specific disease or therapeutic area. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Investigations of Early Nutrition Effects on Long-Term Health: Methods and Applications aim is to provide insights into the latest ideas and technologies .enabling progress in this field
    Pages: XIV, 520 p. 101 illus., 88 illus. in color. : online resource.
    ISBN: 9781493976140
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  • 3
    Keywords: Toxicology ; Laboratory animals ; Pharmacology/Toxicology ; Animal Models ; Springer eBooks
    Description / Table of Contents: A Guide to Mass Spectrometry-based Quantitative Proteomics -- Mitochondrial Involvement in Mental Disorders; Energy Metabolism, Genetic and Environmental Factors -- Monosodium Glutamate (MSG)-induced Animal Model of Type 2 Diabetes -- The Elevated Plus Maze Test for Measuring Anxiety-like Behaviour in Rodents -- The Forced Swim Test for Depression-like Behaviour in Rodents -- Object Burying Test for Assessment of Obsessive Compulsive Behaviours in Mice -- The Nest Building Test in Mice for Assessment of General Well-being -- Mouse Free Dyadic Social Interaction Test -- The Open Field Test for Measuring Locomotor Activity and Anxiety-Like Behaviour -- The Y-maze for Assessment of Spatial Working and Reference Memory in Mice -- Maturation of a Human Oligodendrocyte Cell Line -- Monitoring Age-related Changes in the Lactate/pyruvate Ratio using a Colorimetric Assay in a C. elegans Model of Increased Lifespan -- Isolation and Characterisation of Different Mesenchymal Stem Cell Populations from Rat Femur -- Characterization of an Animal Model of Autism and Social Interaction -- Assaying Reproductive Capacity in Female Rodents -- Experimental Model of Hindlimb Suspension-Induced Skeletal Muscle Atrophy in Rodents -- An Analysis of the Intracellular Signal Transduction of Peripheral Blood Leukocytes in Animal Models of Diabetes using Flow Cytometry -- Characterization of the db/db Mouse Model of Type 2 Diabetes -- Characterization of the Goto-Kakizaki (GK) Rat Model of Type 2 Diabetes -- Characterization of Human Transplantable Insulinoma Cells -- Preparation of Islets from Rat Pancreas and Assessment of Islet Function -- Cryopreservation of Red Blood Cells -- Molecular Characterization of Human Leukemia 60 (HL-60) Cells as a Model of Acute Myelogenous Leukemia Post Cryo-preservation -- Reprogramming of Human Melanocytes and Melanoma cells with Yamanaka Factors -- The Use of 3T3-L1 Murine Preadipocytes as a Model of Adipogenesis -- Generation of Human Pyruvate Carboxylase Knockout Cell Lines Using Retrovirus Expressing Short Hairpin RNA and CRISPR-Cas9 as Models to Study its Metabolic Role in Cancer Research -- The use of Primary Hepatocytes in Assessment of Drug Safety and Toxicity -- OVA-induced Allergic Airway Inflammation Mouse Model -- Description of Ovariectomy Protocol in Mice -- A Rat Eye Lens Model of Cataract Formation -- A Complete Proteomic Workflow to Study Brain-Related Disorders via Postmortem Tissue -- Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 β-Cell Line– a Preclinical Model for Type 2 Diabetes
    Abstract: This volume details reviews and protocols on the development and analysis of both cellular and animal-based pre-clinical models in a number of medical areas, including metabolic disorders, longevity, cancer, heart disease and psychiatric disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Pre-Clinical Models: Techniques and Protocols aims to provide methods that describe the context of specific disease or therapeutic areas
    Pages: XIII, 343 p. 90 illus., 73 illus. in color. : online resource.
    ISBN: 9781493989942
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  • 4
    Keywords: Medicine ; Pharmacology ; Biomedicine ; Pharmacology/Toxicology ; Springer eBooks
    Description / Table of Contents: Application of Multiplex Biomarker Approaches to Accelerate Drug Discovery and Development -- The Application of Multiplex Biomarker Techniques for Improved Stratification and Treatment of Schizophrenia Patients -- Multiplex Biomarker Approaches in Type 2 Diabetes Mellitus Research -- LC-MSE, Multiplex MS/MS, Ion Mobility, and Label-Free Quantitation in Clinical Proteomics -- Phenotyping Multiple Subsets of Immune Cells In Situ in FFPE Tissue Sections: An Overview of Methodologies -- Identification and Clinical Translation of Biomarker Signatures: Statistical Considerations -- Opportunities and Challenges of Multiplex Assays: A Machine Learning Perspective -- Multiplex Analyses Using Real-Time Quantitative PCR -- Multiplex Analysis Using cDNA Transcriptomic Profiling -- Multiplex Single Nucleotide Polymorphism Analyses -- Pulsed SILAC as a Proteomic Approach for miRNA Targets Identification in Cell Culture -- Blood Bio-Sampling Procedures for Multiplex Biomarkers Studies -- Multiplex Immunoassay Profiling -- Multiplex Sequential Immunopreciptation of Insulin Secretory Granule Proteins from Radiolabeled Pancreatic Islets -- Two Dimensional Gel Electrophoresis of Insulin Secretory Granule Proteins from Biosynthetically-Labeled Pancreatic Islets -- Depletion of Highly Abundant Proteins of the Human Blood Plasma: Applications in Proteomics Studies of Psychiatric Disorders -- Simultaneous Two-Dimensional Difference Gel Electrophoresis (2D-DIGE) Analysis of Three Distinct Proteomes -- Selective Reaction Monitoring for Quantitation of Cellular Proteins -- Characterization of a Protein Interactome by Co-Immunoprecipitation and Shotgun Mass Spectrometry -- Using 15N-Metabolic Labeling for Quantitative Proteomic Analyses -- Multiplex Measurement of Serum Folate Vitamers by UPLC-MS/MS -- UPLC-MS/MS Determination of Deuterated 25-Hydroxyvitamin D (d3-25OHD3) and Other Vitamin D Metabolites for the Measurement of 25OHD Half-Life -- iTRAQ-Based Shotgun Proteomics Approach for Relative Protein Quantification -- 1H NMR Metabolomic Profiling of Human and Animal Blood Serum Samples -- Lab-on-a-Chip Multiplex Assays -- Multiplex Smartphone Diagnostics -- Development of a User-Friendly App for Assisting Anticoagulation Treatment -- Multiplex Biomarker Approaches to Enable Point-of-Care Testing and Personalized Medicine
    Abstract: This detailed book arrives as there is an increasing need for multiplex biomarker readouts for improved clinical management and to support the development of new drugs by pharmaceutical companies, due to continuous technical developments and new insights into the high complexity of many diseases. Chapters explore the basic technology platforms being applied in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging, which are currently the methods of choice in multiplex biomarker research. The book also describes the chief medical areas in which the greatest progress has been made and highlight areas where further resources are required. Written for the highly successful Methods in Molecular Biology series, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 〈 Authoritative and invaluable, Multiplex Biomarker Techniques: Methods and Applications serves as an ideal resource for a wide variety of researchers interested in these vital multiplex techniques
    Pages: XV, 317 p. 59 illus., 48 illus. in color. : online resource.
    ISBN: 9781493967308
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  • 5
    Cham : Springer International Publishing
    Keywords: Life sciences ; Gene Expression ; Gene Therapy ; Proteomics ; Life sciences ; Proteomics ; Gene Expression ; Gene Therapy ; Springer eBooks
    Description / Table of Contents: Preface – Proteomic studies in psychiatric disease and neurological disorders -- Part 1: REVIEWS -- Application of proteomic techniques for improved stratification and treatment of schizophrenia patients -- Multiplexing biomarker methods, proteomics and considerations for Alzheimer’s disease -- What have proteomic studies taught us about novel drug targets in autism? -- Application of proteomic approaches to accelerate drug development for psychiatric disorders -- Proteomic biomarker identification in cerebrospinal fluid for leptomeningeal metastases with neurological complications -- Connecting brain proteomics with behavioural neuroscience in translational animal models of neuropsychiatric disorders -- LC-MSE for qualitative and quantitative proteomicstudies of psychiatric disorders -- The utility of multiplex assays for identification of proteomic signatures in psychiatry -- Part 2: PROTOCOLS -- Blood sampling and preparation procedures for proteomic biomarker studies of psychiatric disorders -- Multiplex immunoassay profiling of serum in psychiatric disorders -- Sequential immunopreciptation of secretory vesicle proteins from biosynthetically-labelled cells -- 2d gel electrophoresis of insulin secretory granule proteins from biosynthetically-labelled pancreatic islets -- Two-dimensional gel electrophoresis: a reference protocol -- A two-dimensional difference gel electrophoresis (2D-DIGE) protocol for studies of neural precursor cells -- Identifying biomarker candidates in the blood plasma or serum proteome -- Selective reaction monitoring mass spectrometry for quantitation of glycolytic enzymes in post-mortem brain samples -- A selected reaction monitoring mass spectrometry protocol for validation of proteomic biomarker candidates in studies of psychiatric disorders -- Application of iTRAQ shotgun proteomics for measurement of brain proteins in studies of psychiatric disorders -- Co-immunoprecipitation for deciphering protein interactomes -- Sequential co-immunopreciptation and immunoblot approach to determine oligomerisation of G-protein coupled receptors -- A protocol for producing the maternal low protein rat model: a tool for pre-clinical proteomic studies -- A clinical study protocol to identify serum biomarkers predictive of response to antipsychotics in schizophrenia patients -- Generation of the acute phencyclidine rat model for proteomic studies of schizophrenia A protocol for generation of a corticosterone model of psychiatric disorders -- MK-801-treated oligodendrocytes as a cellular model to study schizophrenia -- Combining patient-reprogrammed neural cells and proteomics as a model to study psychiatric disorders -- SILAC mass spectrometry profiling: a psychiatric disorder perspective -- Preparation of peripheral blood mononuclear cells (PBMCs) as a model for proteomic studies psychiatric disorders -- Proteomic profiling of skin fibroblasts as a model of schizophrenia Proteomic profiling of the pituitary gland in studies of psychiatric disorders -- Development of an assay for measuring proprotein-conversion activity on a multiplex magnetic bead-based array platform -- Phenotyping multiple subsets of immune cells in situ in formalin-fixed, paraffin-embedded tissue sections -- Lab-on-a-chip proteomic assays for psychiatric disorders -- Development of a user-friendly app for testing blood coagulation status in schizophrenia patients -- Part 3:FUTURE PERSPECTIVES -- Proteomic approaches to enable point-of-care testing and personalized medicine for psychiatric disorders
    Abstract: Due to continuous technical developments and new insights into the high complexity of neurological diseases, there is an increasing need for the application of proteomic technologies which can yield potential biomarker readouts for improved clinical management as well as for the development of new drugs by struggling pharmaceutical companies. This book describes the step-by-step use of proteomic methods such as two-dimensional gel electrophoresis, multiplex immunoassay, liquid chromatography mass spectrometry (LC-MS) and selective reaction monitoring MS, to increase our understanding of these diseases, with the ultimate aim of improving patient care. The volume will be of high interest to clinical scientists, physicians and pharmaceutical company scientists as it gives insights into the latest technologies enabling the revolution of personalized medicine. It is of direct interest to both technical and bench biomarker scientists as it gives step by step instructions on how to carry out each of the protocols. It is also of interest to researchers as each technique will be presented in the context of a specific neurological disorder, including Alzheimer's disease, multiple sclerosis, autism spectrum disorders, schizophrenia, major depressive disorder and bipolar disorder. Finally, it will also highlight the future research efforts in this field, which are endeavoring to convert proteomic platforms to the form of hand held devices which can be used in a point of care setting and return diagnostic results within the timeframe of a visit to the general practitioner
    Pages: XII, 370 p. 55 illus., 45 illus. in color. : online resource.
    ISBN: 9783319524795
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  • 6
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: A variant of the PC12 pheochromocytoma cell line (termed A35C) has been isolated that lacks regulated secretory organelles and several constituent proteins. Northern and Southern blot analyses suggested a block at the transcriptional level. The proprotein-converting enzyme carboxypeptidase H was synthesised in the A35C cell line but was secreted by the constitutive pathway. Transient transfection of A35C cells with cDNAs encoding the regulated secretory proteins dopamine β-hydroxylase and synaptotagmin I resulted in distinct patterns of mistargeting of these proteins. It is surprising that hybrid cells created by fusing normal PC12 cells with A35C cells exhibited the variant phenotype, suggesting that A35C cells express an inhibitory factor that represses neuroendocrine-specific gene expression.
    Type of Medium: Electronic Resource
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