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  • 1
    Keywords: Life sciences ; Plant science ; Botany ; Life sciences ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: An Overview of Methods in Plant Nitric Oxide (NO) Research: Why Do We Always Need to Use Multiple Methods? -- Chemiluminescence Detection of Nitric Oxide from Roots, Leaves, and Root Mitochondria -- Nitric Oxide Measurement from Purified Enzymes and Estimation of Scavenging Activity by Gas Phase Chemlimunimescence Method -- Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence -- Nitric Oxide (NO) Measurements in Stomatal Guard Cells -- Methods to Detect Nitric Oxide in Plants: Are DAFs Really Measuring NO? -- A Novel Protocol for Detection of Nitric Oxide in Plants -- Detection of Nitric Oxide by Electron Paramagnetic Resonance Spectroscopy: Spin-Trapping with Iron-Dithiocarbamates -- Measurement of Nitric Oxide (NO) Generation Rate by Chloroplasts Employing Electron Spin Resonance (ESR) -- Laser-Based Methods for Detection of Nitric Oxide in Plants -- Electrochemical Detection of Nitric Oxide in Plant Cell Suspensions -- Quantification and Localization of S-Nitrosothiols (SNOs) in Higher Plants -- Analysis of the Expression and Activity of Nitric Oxide Synthase from Marine Photosynthetic Microorganisms -- Identification of S-Nitrosothiols by the Sequential Cysteine Blocking Technique -- Detection of S-Nitrosoglutathione Reductase Activity in Plants -- Detection of Peroxynitrite in Plants Exposed to Bacterial Infection
    Abstract: With numerous methods available for the quantification of nitric oxide (NO), this detailed book explores their advantages and disadvantages in order to provide a foundation for further research in plant nitric oxide. After providing a useful practical guide to choosing a technique for measuring NO from plant materials, the book continues with chapters on chemiluminescence, diaminofluorescence (DAF), EPR spectroscopy, a laser-based method, as well as many other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory methods, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Nitric Oxide: Methods and Protocols serves as a valuable guide to all researchers working and intending to work in the field of plant nitric oxide research
    Pages: XI, 203 p. 54 illus., 34 illus. in color. : online resource.
    ISBN: 9781493936007
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  • 2
    Keywords: Life sciences ; Biochemistry ; Cytology ; Plant physiology ; Life sciences ; Plant physiology ; Oxidative Stress ; Plant Biochemistry ; Springer eBooks
    Description / Table of Contents: Compartmentalization of Reactive Oxygen Species and Nitric Oxide Production in Plant Cells – An Overview -- Established and Proposed Roles of Xanthine Oxidoreductase in Oxidative and Reductive Pathways in Plants -- The Roles of Plant Peroxidases in the Metabolism of Reactive Nitrogen Species and Other Nitrogenous Compounds -- Mitochondrial Signaling in Plants Under Hypoxia: Use of Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS).-Feedback Loop of Non-Coupled Respiration and Reactive Oxygen Species Production in Plant Mitochondria -- Antioxidative Systems and Stress Tolerance - Insight from Wild and Cultivated Tomato Species -- The Role of Reactive Oxygen Species Under Ammonium Nutrition -- Allelopathic Compounds as Oxidative Stress Agents: Yes or NO -- The Role of Reactive Oxygen and Nitrogen Species in Bioenergetics, Metabolism and Signaling During Seed Germination -- ROS Signalling in Plant Embryogenesis.- “Nitrosative Door” in Seed Dormancy Alleviation and Germination -- Dissecting Nitric Oxide Signaling in Nucleus: Role of S-Nitrosylation in Regulating Nuclear Proteins -- Nitration and S-Nitrosylation: Two Post-Translational Modifications (Ptms) Mediated by Reactive Nitrogen Species (RNS) and Their Role in Signalling Processes of Plant Cells -- S-Nitrosoglutathione Reductase: A Key Regulator of S-Nitrosylation in Plant Development and Stress Responses -- Interaction of Calcium Signaling With Reactive Oxygen and Reactive Nitrogen Species
    Abstract: This book reviews the current state of information on reactive oxygen and nitrogen species and their role in cell communication during plant growth, development and adaptation to stress conditions. It addresses current research advances made in the area of reactive oxygen and nitrogen species (ROS and RNS) signaling. These free radical molecules are important in plant-microbe interactions, responses to abiotic stress, stomatal regulation and a range of developmental processes. Due to their short half-life, high diffusion capability and ability to react with different components in the cell, ROS and RNS participate in various processes connected with signaling and communication in plants. The book’s respective chapters address the latest advances made in the niche area of ROS and RNS in plants. It offers a valuable guide for researchers and students alike, providing insights into cutting-edge free radical research. The information on specialized topics presented is also highly relevant for applied fields such as food security, agricultural practices and medicinal use of plants
    Pages: VIII, 316 p. 171 illus., 157 illus. in color. : online resource.
    ISBN: 9783319100791
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  • 3
    Keywords: Plant science ; Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: An Overview of Important Enzymes Involved in Nitrogen Assimilation of Plants -- Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues -- Measurement of Nitrate Reductase Activity in Tomato (Solanum lycopersicum L.) Leaves under Different Conditions -- Fluorimetric-Based Method to Detect and Quantify Total S-Nitrosothiols (SNOs) in Plant Samples -- Measurement of S-Nitrosoglutathione Reductase Activity in Plants -- Expression Analysis of Important Genes Involved in Nitrogen Metabolism under Hypoxia -- Enzymatic Conversions of Glutamate and γ-Aminobutyric Acid as the Indicators of Plant Stress Response -- Using Different Forms of Nitrogen to Study Hypersensitive Response Elicited by Avirulent Pseudomonas syringae -- Using Foldscope to Monitor Superoxide Production and Cell Death during Pathogen Infection in Arabidopsis under Different Nitrogen Regimes -- Methods for Estimation of Nitrogen Components in Plants and Microorganisms -- A Precise Method for Analysing Nitrogen Use in Foxtail Millet -- Methods for Isolation and Characterization of Nitrogen Fixing Legume-Nodulating Bacteria -- Detection and Quantification of Nitrifying Bacteria using Real-Time PCR -- Extracellular Matrix Proteome: Isolation of ECM Proteins for Proteomics Studies
    Abstract: This volume explores several different aspects of nitrogen metabolism, ranging from nitrogen uptake to assimilation. The chapters in this book cover topics such as measurement of activities of enzymes involved in nitrogen metabolism (i.e., nitrate reductase); measurement of nitric oxide, nitrite and ways to detect the N content in plants and microbes; characterization of root nodule bacteria (RNB); and techniques to perform proteomics of Extracellular Matrix (ECM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Nitrogen Metabolism in Plants: Methods and Protocols is a valuable tool for novice and expert researchers who are interested in learning more about this evolving field
    Pages: X, 178 p. 41 illus., 32 illus. in color. : online resource.
    Edition: 1st ed. 2020.
    ISBN: 9781493997909
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