Description / Table of Contents:
An Overview of Methods in Plant Nitric Oxide (NO) Research: Why Do We Always Need to Use Multiple Methods? -- Chemiluminescence Detection of Nitric Oxide from Roots, Leaves, and Root Mitochondria -- Nitric Oxide Measurement from Purified Enzymes and Estimation of Scavenging Activity by Gas Phase Chemlimunimescence Method -- Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence -- Nitric Oxide (NO) Measurements in Stomatal Guard Cells -- Methods to Detect Nitric Oxide in Plants: Are DAFs Really Measuring NO? -- A Novel Protocol for Detection of Nitric Oxide in Plants -- Detection of Nitric Oxide by Electron Paramagnetic Resonance Spectroscopy: Spin-Trapping with Iron-Dithiocarbamates -- Measurement of Nitric Oxide (NO) Generation Rate by Chloroplasts Employing Electron Spin Resonance (ESR) -- Laser-Based Methods for Detection of Nitric Oxide in Plants -- Electrochemical Detection of Nitric Oxide in Plant Cell Suspensions -- Quantification and Localization of S-Nitrosothiols (SNOs) in Higher Plants -- Analysis of the Expression and Activity of Nitric Oxide Synthase from Marine Photosynthetic Microorganisms -- Identification of S-Nitrosothiols by the Sequential Cysteine Blocking Technique -- Detection of S-Nitrosoglutathione Reductase Activity in Plants -- Detection of Peroxynitrite in Plants Exposed to Bacterial Infection
With numerous methods available for the quantification of nitric oxide (NO), this detailed book explores their advantages and disadvantages in order to provide a foundation for further research in plant nitric oxide. After providing a useful practical guide to choosing a technique for measuring NO from plant materials, the book continues with chapters on chemiluminescence, diaminofluorescence (DAF), EPR spectroscopy, a laser-based method, as well as many other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory methods, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Nitric Oxide: Methods and Protocols serves as a valuable guide to all researchers working and intending to work in the field of plant nitric oxide research
XI, 203 p. 54 illus., 34 illus. in color. : online resource.