Life Sciences (general)
Wiley InterScience Backfile Collection 1832-2000
Process Engineering, Biotechnology, Nutrition Technology
The effect of an enzyme preparation of Streptomyces spec. KT3 was tested in view of its cell wall lytic activity on cells of Saccharomyces cerevisiae. According to electron micrographs under the influence of the enzyme preparation protoplasts but no spheroplasts were formed. The yield of protoplasts amounted dependent on the enzyme charge to 75-99 per cent, most of the protoplasts were released after 60 minutes. Cells of 12 strains of Saccharomyces cerevisiae were able to be protoplasted and stabilized immediately thereafter by mannitol (0.8 M) or KCl (0.6 M). The presence of stabilizers already in the enzyme-containing medium inhibited, however, protoplasting considerably. The stabilized protoplasts could be stored for six days at 4°C without any noticeable changes.
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