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  • 1
    ISSN: 0167-7799
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Trends in Biotechnology 3 (1985), S. 73-79 
    ISSN: 0167-7799
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Trends in Biotechnology 11 (1993), S. 317-319 
    ISSN: 0167-7799
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0304-4165
    Keywords: Adaptation ; Continuous culture ; E. coli ; Kinetics ; Non-equilibrium thermodynamics ; Oligotrophy ; Sugar analysis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-072X
    Keywords: Temperature ; Heat shock gene expression ; htpG ; Heat shock protein ; Escherichia coli ; Continuous culture ; Dilution rate ; Growth medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Most of the data concerning heat shock gene expression reported in the literature are derived from batch culture experiments under substrate and nutrient sufficient conditions. Here, the effects of dilution rate and medium composition on the steady state and heat shock induced htpG gene expression have been investigated in continuous cultures of Escherichia coli, using a chromosomal htpG-lacZ gene fusion. During steady state growth temperature dependent patterns of the relative htpG expression were found to be largely similar, irrespective of the growth condition. However, nitrogen-limited growth resulted in a markedly reduced specific steady state htpG expression as compared to growth under carbon limitation or in complex medium, correlating qualitatively with the total cellular protein content. During heat shock, tight temperature controlled expression was evident. While the relative heat shock induced expression was largely identical at various dilution rates in a given growth medium, significantly different response patterns were observed in the three growth media at any give dilution rate. From these results a clearly temperature regulated htpG expression during both, steady and transient state growth in continuous culture is evident, which is further significantly affected by the growth condition used.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The effect of temperature on a population of Klebsiella pneumoniae was examined together with the imposition of mild starvation conditions at temperatures of 35°, 41°, 49°, 55° and 60 °C. Results for changes in biomass, protein and metabolic activity are presented in terms of gross population changes and show that these parameters decline with increasing temperature. Increases in the amount of dissolved organic carbon, together with a decrease in the number of cells present with increasing temperature, suggest that death and lysis processes are occurring. Regrowth in the bioreactor occurred after returning the temperature to 35 °C and starting a flow of carbon and other nutrients. This was probably due to reinoculation from head space wall growth drainage rather than reversion of heat-stressed microbes. The consequences of this for thermophilic sludge treatment processes are discussed. The concept of endogenous metabolism is questioned with respect to it being a realistic description of the survival process.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary In large industrial scale bioreactors process cultures are frequently exposed to supperoptimal temperatures for short intervals of time. Data concerning the physiologica effects of such exposures are scant. Here, experiments involving short term heat shocks on two bacteria, Klebsiella pneumoniae, a non-fastidious mesophile growing on glucose and Bacillus sp. NCIB 12522, a fastidious thermotolerant methylotroph growing on methanol, are described. Markedly different response patterns for the two bacteria are evident, clearly indicating not only the dangers of making generalizations with respect to the effects of superoptimal temperatures on growing bacterial cultures but also, the significance of scale related segregation phenomena such as wall growth.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The solubilization and biodegradation of whole microbial cells by an aerobic thermophilic microbial population was investigated over a 72 h period. Various parameters were followed including total suspended solids reduction, changes in the dissolved organic carbon, protein and carbohydrate concentrations, and carboxylic acid production and utilisation. From the rates of removal of the various fractions a simple model for the biodegradation processes is proposed and verified with respect to acetic acid production and utilization, and total suspended solids removal. The process is initiated by enzymic degradation of the substrate microbe cell walls followed by growth on the released soluble substrates at low dissolved oxygen concentration with concommitant carboxylic acid production. Subsequent utilization of the unbranched, lower molecular weight carboxylic acids allows additional energy supply following exhaustion of the easily utilisable soluble substrate from microbial cell hydrolysis.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The introduction of more imaginative enrichment and isolation procedures has permitted the isolation of pure cultures of thermotolerant methylotrophic bacteria, a group that was previously unknown. One potential application for such bacteria is in the aerobic biotreatment of petrochemical industry wastewaters at elevated temperatures. Here, the growth and biooxidation characteristics of one such bacterium, Bacillus sp. NCIB 12522, in co-culture with a Gram-negative thermophilic non-methylotrophic solvent utilizing bacterium, NA 17, on a mixture of methanol, ethanol and isopropanol, under both steady and transient state continuous flow culture conditions are reported. The results indicate that at dilution rates 〈0.2 h−1 effective biooxidation can be achieved at temperatures between 50° and 57 °C. As a result of step increases in bioreactor feed concentrations, the fraction of the methylotroph present in the co-culture changed according to whether the methanol or the ethanol concentrations were increased, but when isopropanol was increased, no change in the methylotroph fraction occurred between the initial and final steady states.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The work reported concerns the removal of mixtures of two ketones, methyl ethyl ketone (MEK) and methyl isobutyl ketone (MIBK), which find wide application as industrial solvents, from effluent air streams in downward flow biofilters operating at relative humidities in excess of 95 percent. The inlet concentrations of the two pollutants were 300 mg m−3 MEK and 330 mg m−3 MIBK. Maximum elimination capacities achieved were 50 g m−3h−1 for MEK and 20 g m−3h−1 for MIBK. Marked interaction between the elimination of the two ketones was observed and established biophysical models for the kinetic analysis of biofilter operation proved inadequate as far as the complex processes involved in multi-component biodegradable vapour elimination were concerned. The complexity of such systems requires further definition and the development of appropriate models for process evaluation and design.
    Type of Medium: Electronic Resource
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